Effects of ovariectomy and resistance training on MMP-2 activity in rat calcaneal tendon

Pereira, Guilherme Borges; Prestes, Jonato; Leite, Richard Diego; Magosso, Rodrigo Ferro; Peixoto, Fabiana Sobral; Marqueti, Rita de Cássia; Shiguemoto, Gilberto Eiji; Selistre-de-Araújo, Heloísa Sobreiro; Baldissera, Vilmar; Perez, Sérgio Eduardo de Andrade

doi:10.3109/03008201003676330

Cited by 16 publications

(19 citation statements)

References 35 publications

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“…Ovariectomy downregulates MMP-2 in TA and EDL, which may compromise skeletal muscle function. Additionally, Pereira et al [ 28 ] also demonstrated that the absence of ovarian hormones alters MMP-2 activity in the calcaneal tendon, mainly in the proximal region, indicating a diff erentiated tissue remodeling. It has also been shown that ovariectomy prevents the recovery of the cross-sectional area of soleus muscle fi bers after disuse atrophy during 2 weeks of normal cage ambulation, while estrogen replacement allows for the restoration of muscle fi ber size [ 17 ] .…”

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confidence: 94%

Resistance Training and Glycogen Content in Ovariectomized Rats

et al. 2012

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The aim of the present study was to investigate the effects of resistance training on glycogen content and muscle cross-sectional area (CSA) in ovariectomized rats. Wistar rats were divided into: sedentary; ovariectomized sedentary; resistance trained; and ovariectomized resistance trained. In the 12-week resistance training, the animals climbed a 1.1 m vertical ladder, 3 days per week, with 4-8 climbs. Cardiac, liver and muscle glycogen content was determined. After the 12-week resistance training period there was a higher hepatic and muscle glycogen content in the resistance training group compared with the other groups (p<0.01). CSA was higher in soleus for the resistance trained, ovariectomized resistance trained and sedentary compared with ovariectomized sedentary (p<0.05). Ovariectomy attenuated the increase in liver and muscle glycogen content, while soleus muscle cross-sectional area increased with resistance training, even in ovariectomized rats. Resistance training could be an important exercise to increase muscle function in situations of reduced estrogen and progesterone.

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confidence: 94%

Resistance Training and Glycogen Content in Ovariectomized Rats

et al. 2012

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“…A housing chamber (L × W × H = 20 × 20 × 20 cm) was located at the top of the ladder and served as a shelter during the resting period ( Fig. 1a) [23,[26][27][28][29][30]. The familiarization protocol consisted of three trials per day for three days.…”

Section: Familiarization With the Vertical Laddermentioning

confidence: 99%

A strength exercise program in rats with epilepsy is protective against seizures

Peixinho-Pena

Fernandes

Almeida

et al. 2012

Epilepsy & Behavior

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The beneficial effects of physical exercise on epilepsy, such as a decreased seizure frequency, have been observed following aerobic exercise programs in both clinical and experimental studies. However, it is not well clarified whether other types of exercise, including strength exercise, can provide similar benefits for epilepsy. Forty four animals with epilepsy were continuously monitored 24 h a day for 60 days and divided into two periods of 30 days. The first period was used to determine the number of seizures before beginning the physical exercise program, and the second period was utilized to determine the number of seizures during the strength training. The mean frequency of seizures in the control and SHAM groups increased significantly from period 1 to period 2. Although the frequency of seizures did not change significantly between the two periods of 30 days of observation in the strength exercise group, a significant reduction in the seizure frequency was observed compared with the control and SHAM groups in period 2. Our study demonstrated that a strength exercise program exerted a significant influence on the seizure frequency in animals with epilepsy and strengthens the observed beneficial effect of exercise on epilepsy that has been demonstrated in animal studies. The finding of this nonclinical study can open a new window to verify the beneficial contribution of strength exercise in epilepsy. Further experimental and clinical investigations are necessary to explore the extent to which strength exercise interferes with the epileptic condition.

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“…The training protocol consisted of progressive REx, 5 times/wk during 8 wk [adapted from [17][18][19]. A vertical ladder (110 cm high by 18 cm wide, inclined at 80º with 2 cm spacing between rungs) was used; at the top of the ladder there was a dark box (20 cm × 20 cm ×20 cm), where the animal could rest between sets (1 min).…”

Section: Exercise Training and Drug Treatmentmentioning

confidence: 99%

Validation of Reference Genes for qPCR Analysis of Resistance Training and Androgenic Anabolic Steroids on Hypothalamus, Adrenal Gland and Fat Tissue

Pozzi¹,

Le²,

Fern³

et al. 2016

J Steroids Horm Sci

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Background: Real-time quantitative Polymerase Chain Reaction (qPCR) is a technique used for quantification of gene expression and the use of reference genes is very important to normalize the quantification results. Aim:To validate the most suitable reference genes for resistance exercise training (REx) and use of nandrolone decanoate (DECA) in three different rat tissues. Methods:A total of 40 adult male Wistar rats were distributed into four groups: exposed to vehicle three times per week (wk) (CT); eight wk of REx exposed to vehicle three times per wk (T); exposed to DECA three times per wk (D); eight wk of REx exposed to DECA three times per wk (TD). Stability of the following genes was evaluated: beta actin (Actb), alpha Tubulin (Tubulin), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Hypoxanthine phosphoribosyltransferase-1 (Hprt1) and 18s Ribossomal RNA (18s) in hypothalamus, adrenal gland and mesenteric fat tissue using GeNorm, NormFinder and BestKeeper software. Results:In hypothalamus and adrenal, all genes were suitable and none was rejected by statistical analysis; however, in fat tissue, Actb, Gapdh and Hprt1 genes were rejected by geNorm but not the others two software. Conclusion:In hypothalamus and adrenal all selected genes analized were stable and can be used for qPCR gene expression analysis. However, in fat tissue we suggest the Tubulin gene as most stable gene.

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Effects of ovariectomy and resistance training on MMP-2 activity in rat calcaneal tendon

Cited by 16 publications

References 35 publications

Resistance Training and Glycogen Content in Ovariectomized Rats

Resistance Training and Glycogen Content in Ovariectomized Rats

A strength exercise program in rats with epilepsy is protective against seizures

Validation of Reference Genes for qPCR Analysis of Resistance Training and Androgenic Anabolic Steroids on Hypothalamus, Adrenal Gland and Fat Tissue

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