Effects of Ovariectomy and Estrogen Replacement Therapy on Laryngeal Tissue

Tatlipinar, Arzu; Güneş, Pembegül; Özbeyli, Dilek; Çimen, Burak; Gökçeer, Tanju

doi:10.1177/0194599811423638

Cited by 11 publications

(6 citation statements)

References 16 publications

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“…This observation is also consistent with the loss of cilia induced by estrogen deficiency in the laryngeal mucosa of rats [41]. We found that the amount of MC cells is also increased by T and EE2 (Figure 5A-D), It has been shown that a T enhances ciliogenesis and MC cell amounts in female gerbils prostate mucosa [20], and that a natural increase of T and E2 is linked to more MC cells in oviduct epithelia of tetrapods [16].…”

Section: Discussionsupporting

confidence: 88%

Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context

Castillo-Briceño

Kodjabachian

2014

Front Zool

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BackgroundHow important are sexual hormones beyond their function in reproductive biology has yet to be understood. In this study, we analyzed the effects of sex steroids on the biology of the embryonic amphibian epidermis, which represents an easily amenable model of non-reproductive mucociliary epithelia (MCE). MCE are integrated systems formed by multiciliated (MC), mucus-secreting (MS) and mitochondrion-rich (MR) cell populations that are shaped by their microenvironment. Therefore, MCE could be considered as ecosystems at the cellular scale, found in a wide array of contexts from mussel gills to mammalian oviduct.ResultsWe showed that the natural estrogen (estradiol, E2) and androgen (testosterone, T) as well as the synthetic estrogen (ethinyl-estradiol, EE2), all induced a significant enhancement of MC cell numbers. The effect of E2, T and EE2 extended to the MS and MR cell populations, to varying degrees. They also modified the expression profile of RNA MCE markers, and induced a range of “non-typical” cellular phenotypes, with mixed identities and aberrant morphologies, as revealed by imaging analysis through biomarker confocal detection and scanning electron microscopy. Finally, these hormones also affected tadpole pigmentation, revealing an effect on the entire cellular ecosystem of the Xenopus embryonic skin.ConclusionsThis study reveals the impact in vivo, at the molecular, cellular, tissue and organism levels, of sex steroids on non-reproductive mucociliary epithelium biogenesis, and validates the use of Xenopus as a relevant model system in this field.

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Section: Discussionsupporting

confidence: 88%

Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context

Castillo-Briceño

Kodjabachian

2014

Front Zool

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“…In female rats, sex hormones are critical to homeostasis of the pre-menopausal vocal fold mucosal tissues [64][65][66]. Several studies have demonstrated that removal of the ovaries (elimination of ovarian hormones) results in the remodeling of the vocal fold mucosa including the following: decreased cellular layers of the epithelium, increased edema of the lamina propria, and decreased collagen I, hyaluronic acid, and elastin of the lamina propria [64][65][66]. Although ovariectomy procedures have demonstrated vocal fold mucosal remodeling, orchiectomy procedures in male rats have not resulted in significant remodeling [64].…”

Section: Sex Differences In Vocal Fold Mucosamentioning

confidence: 99%

Biological and Acoustic Sex Differences in Rat Ultrasonic Vocalization

et al. 2021

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The rat model is a useful tool for understanding peripheral and central mechanisms of laryngeal biology. Rats produce ultrasonic vocalizations (USVs) that have communicative intent and are altered by experimental conditions such as social environment, stress, diet, drugs, age, and neurological diseases, validating the rat model’s utility for studying communication and related deficits. Sex differences are apparent in both the rat larynx and USV acoustics and are differentially affected by experimental conditions. Therefore, the purpose of this review paper is to highlight the known sex differences in rat USV production, acoustics, and laryngeal biology detailed in the literature across the lifespan.

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“…Loss of estrogen through surgically induced menopause in female rats increased vocal fold inflammation, edema, and vasodilation, and eliminated vocalizations during mating. 11,12 However, the effects of estrogen on the muscular portion of the vocal fold is unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Evidence from the rat model suggests that sex hormones affect the outer vibratory layer and, consequently, vocal fold function. Loss of estrogen through surgically induced menopause in female rats increased vocal fold inflammation, edema, and vasodilation, and eliminated vocalizations during mating 11,12 . However, the effects of estrogen on the muscular portion of the vocal fold is unknown.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Menopause on Neuromuscular Parameters of the Rat Vocal Folds

Lenell

Johnson

2020

The Laryngoscope

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Objectives/Hypothesis: Menopause adversely affecs power and endurance of the limb muscles. However, despite clinical observations that menopause corresponds to negative changes of the voice, the direct effects of estrogen deprivation on the thyroarytenoid muscles are unknown. The purpose of this study was to investigate the effects of estrogen deprivation via ovariectomy on three neuromuscular parameters of the thyroarytenoid muscles using a rat model.Study Design: Animal model. Methods: Cryosections of vocal folds of 20 (10 control and 10 ovariectomized) female rats were stained to label neuromuscular junctions, fiber size, or parvalbumin levels using immunohistochemical techniques and compared between experimental groups.Results: The neuromuscular junctions, thyroarytenoid fiber sizes, and parvalbumin levels of the vocal folds were similar between experimental groups.Conclusions: The loss of estrogen did not change neuromuscular parameters of the vocal folds of adult female rats; therefore, vocal changes within the outer vibratory layers of the vocal folds may primarily be responsible for clinically observed menopausal vocal changes.

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Effects of Ovariectomy and Estrogen Replacement Therapy on Laryngeal Tissue

Abstract: On the basis of histopathological evaluations, it was shown that estrogen replacement helped to improve laryngeal changes due to experimentally induced menopause.

Cited by 11 publications

References 16 publications

Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context

Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context

Biological and Acoustic Sex Differences in Rat Ultrasonic Vocalization

The Effects of Menopause on Neuromuscular Parameters of the Rat Vocal Folds

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