Effects of ouabain on cerebral metabolism and transport mechanisms <i>in vitro</i>

Gonda, O.; Quastel, J. H.

doi:10.1042/bj0840394

Cited by 78 publications

(20 citation statements)

References 39 publications

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“…The cost of molecular transport, for example, undoubtedly varies from system to system and from time to time. Experimental findings, with ouabain, a drug which blocks active transport, have shown that in nervous tissue (Gonda & Quastel, 1962), between 30 and j o yo of basal metabolism may be accounted for in terms of the transport system, and similar results have been obtained for other tissues (Maizels, Remington & Truscoe, 19 58; Paul, 1965). Hence if the upper figure (jo %) were applied generally it would raise basal metabolism by another factor of two from 2164 J to 4328 J.…”

Section: Turnover Of Materials Within Cells Even Bacterial Cells (Eamentioning

confidence: 99%

Conversion Efficiencies in Heterotrophic Organisms

1977

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Summary 1. The maximum possible efficiency at which living systems are able to convert input nutrients to their own biomass is between 70 and 80 %. 2. Conversion efficiency in bacteria, protozoa and metazoan cells in culture approximates more closely to 60%. 3. Conversion efficiency during embryonic development begins below 60% and rises above this level in the later stages. 4. Very young, post‐natal organisms have high net efficiencies; 50 to 70% in homeotherms and 50 to 80 % in poikilotherms. 5. In cellular systems, capable of proliferation, conversion efficiency is independent of food supply. This means that conversion is directly dependent on nutrient supply. 6. Control of growth at the tissue level may occur through the control of the supply of nutrients to the tissues and its entry into the cells. 7. Compensatory growth, after and during undernutrition, involves increased absorption efficiency and reduced metabolic costs.

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Section: Turnover Of Materials Within Cells Even Bacterial Cells (Eamentioning

confidence: 99%

Conversion Efficiencies in Heterotrophic Organisms

1977

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“…The salient features are an increase in oxygen consumption and in the aerobic utilization of glucose to lactic acid. These responses of brain slices to potassium depended upon the concomitant presence of sodium ions, and they were not found when the sodium pump was poisoned with the glycoside, ouabain (Hertz & Clausen, 1963;Gonda & Quastel, 1962;Elliott & Bilodeau, 1962). A further finding is that calcium ions affected the metabolism of brain slices in a way that depended on the sodium and potassium concentrations.…”

Section: Ruajak and R Whittammentioning

confidence: 92%

The metabolic response of brain slices to agents affecting the sodium pump

Ruscák¹,

Whittam²

1967

The Journal of Physiology

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SUMMARY1. Slices of brain cortex from rabbits were incubated in Ringer solution and in Ringer modified by the removal of calcium and sodium, and the addition of ouabain, oligomycin or extra potassium. The potassium content of the tissue, the oxygen consumption and the lactate production from glucose were measured and found to be interrelated.2. Incubation in high-K Ringer caused an increase in oxygen consumption that was prevented by ouabain, oligomycin and deprivation of sodium. Lactate production was also raised, but this increase was unaffected by ouabain and raised further by oligomycin.3. Calcium omission raised metabolism; the tissue K content was unaffected. Oligomycin always decreased oxygen consumption and raised lactate production further. The metabolic responses to calcium, potassium, ouabain and oligomycin depended on sodium.4. After anaerobic incubation, the tissue potassium concentration was still 5 times higher than that in Ringer. It was unaffected by oligomycin but lowered markedly by ouabain.5. The synergistic effects of sodium with potassium, oligomycin, calcium, and calcium plus ouabain suggest that the metabolic responses of brain cortex slices to a high-K Ringer depend on the operation of the sodium pump.

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“…Its mode of action on metabolism, therefore, is very different from that of ouabain, which is also known to suppress electrical stimulation of brain respiration (18) but which, in contrast to TTX, antagonizes potassium-stimulated brain respiration (19).…”

Section: Changes Ttx Inmentioning

confidence: 99%

Regulation of the Adrenal Cortex Secretory Pattern by Adrenocorticotropin

Fevold

1967

Science

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Adrenal gland homogenates from rabbits stimulated for 28 days with adrenocorticotropin have been shown to synthesize cortisol. The total amount of cortisol formed was the same whether progesterone-4-(14)C or Delta(5)-pregnenolone-4-(14)C was used as exogenous substrate. However, when Delta(5)-pregnenolone substrate was used the specific activity of the isolated cortisol was four times as great as when progesterone was used.

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Effects of ouabain on cerebral metabolism and transport mechanisms in vitro

Cited by 78 publications

References 39 publications

Conversion Efficiencies in Heterotrophic Organisms

Conversion Efficiencies in Heterotrophic Organisms

The metabolic response of brain slices to agents affecting the sodium pump

Regulation of the Adrenal Cortex Secretory Pattern by Adrenocorticotropin

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