Effects of Osthole on Migration and Invasion in Breast Cancer Cells

Abstract

Osthole, a natural coumarin derivative, is extracted from the fruit of Cnidium monnieri Cusson. Breast cancer is one of the most commonly diagnosed cancers and the leading cause of death in women. Recent studies have shown that Osthole has anti-tumor activity. However, the effects of Osthole on the migration and invasion of cancer cells have not yet been reported. Here, we found that Osthole is effective in inhibiting the migration and invasion of breast cancer cells by wound healing and transwell assays. Luciferase and zymography assays revealed that Osthole effectively inhibits matrix metalloproteinase-2 promoter and enzyme activity, which might be one of the causes that lead to the inhibition of migration and invasion by Osthole. This is the first report on the inhibitory function of Osthole in migration and invasion in breast cancer cells. Our findings indicate a need for further evaluation of Osthole in breast cancer chemotherapy and chemoprevention.

Crude extract of Euphorbia formosana inhibits the migration and invasion of DU145 human prostate cancer cells: The role of matrix metalloproteinase-2/9 inhibition via the MAPK signaling pathway

“…Metastasis is one of the major causes of cancer-related mortality (31)(32)(33)(34)(35). At present, there is an intense effort to identify potential therapeutic agents that inhibit metastasis (35)(36)(37)(38).…”

“…At present, there is an intense effort to identify potential therapeutic agents that inhibit metastasis (35)(36)(37)(38). We previously demonstrated that CEEF treatment significantly inhibits cell proliferation and induces cell cycle arrest Figure 1.…”

Yellow and green pigments from Calophyllum�inophyllum L. seed oil induce cell death in colon and lung cancer cells

“…Environmental conditions and seasonal changes may affect the incidence of coumarins in various parts of the plant (4). Among coumarins, a number of compounds have been reported to exhibit anticancer properties, including imperatorin (5), esculetin (6) and osthole, which inhibits the migration and invasion of breast cancer cells, potentially via the inhibition of matrix metalloproteinase promoter and enzymatic activity (7). Another type of 4-phenylcoumarins isolated from C. inophyllum is calocoumarin A (8).…”

Pharmacokinetics Analysis of Copen, a Novel Antitumor Semi Synthetic Derivative of Osthole, in Rats after Intragastric and Intravenous Administration

“…Osthole ( Figure 1) is a major bioactive ingredient isolated from Cnidium monnieri (L.) cusson [1,2], which has been used for treatment of pain in female genitalia, impotence, and suppurative dermatitis (as an antipruritogenic agent). Osthole exhibits various pharmacological activities, including in vitro/in vivo antitumor effects [3][4][5][6][7][8], alleviation of hyperglycemia and hypolipidemia [9,10], and has been proposed the possibility of its development as a promising lead compound for drug discovery [11,12]. However, the clinical utility of this phytochemical is limited due to its low bioavailability in vivo [13][14][15][16], and some structural modifications are required for sufficient bioavailability upon oral administration.…”