Effects of osteogenic inducers on cultures of canine mesenchymal stem cells

Abstract

BMP-2 was capable of inducing markers of osteogenesis in short-term cultures of canine MSCs. In MSCs obtained from skeletally immature dogs, ascorbate was required for maximal effects of BMP These results define optimal conditions for stem cell osteogenesis in dogs and will facilitate development of stem cell-based treatments for dogs with fractures.

Isolation, characterization, and in vitro proliferation of canine mesenchymal stem cells derived from bone marrow, adipose tissue, muscle, and periosteum

“…OSTEOPONTIN, also known as bone sialoprotein 1, is expressed in other tissues but is considered an osteoblast-specific marker. 28,51 Osteogenic differentiation and expression of osteogenic transcription factors have not been previously reported for canine MMSCs and PMSCs.…”

“…This was supported by the morphological appearance of nodular aggregates that stained positively with Alizarin red and Von Kossa 20,28,35,50 and the mRNA expression of osteoblast markers. 26,28,51 Alkaline phosphatase, RUNX2, OS-TERIX, and OSTEOPONTIN expression was found in all osteoinduced MSCs. Basal expression of these markers was not detected in MSCs treated with standard culture medium, confirming differentiation down the osteogenic lineage.…”

A comparison of autologous and allogenic bone marrow-derived mesenchymal stem cell transplantation in canine spinal cord injury

“…Cultured mononucleated cells were characterized by fluorescence-assisted cell sorting (FACS) analysis based on previous reports [13,29,37]. For immunophenotypic analysis, third-passage canine MSCs were stained under ice conditions, according to the manufacturer's recommendations regarding the monoclonal antibodies (anti-CD9, anti-CD34, anti-CD44, and anti-CD45; Serotec, USA).…”

“…MSCs are nonhematopoietic progenitor cells that are initially present in bone marrow [7,19,29,37]. Bone marrow-derived MSCs are also known as bone marrow stromal cells and are capable of in vitro differentiation into marrow and non-marrow cell types, such as adipocytes, chondrocytes, osteocytes, myocytes, and neurons [13,17,19,33,37]. A recent study has shown that canine bone marrow-derived MSC can form neurosphere-like clumps and differentiate into neuron-like cells expressing neuronal markers [19,37].…”

The effects of canine bone marrow stromal cells on neuritogenesis from dorsal root ganglion neurons in vitro

“…Veterinary clinical applications also afford an important animal-to-human translation opportunity, especially the pathology of clinical spinal cord injury (SCI) in dogs appears very similar to that documented in humans, as well as in cats and numerous examples of experimental SCI (Smith and Jeffery, 2006). Previous studies (Kadiyala et al 1997;Bruder et al 1998;Arinzeh et al 2003;Volk et al 2005) of canine BMSCs have focused on their osteogenic properties because of the wide clinical applicability. The osteogenic property of canine BMSCs has also been exploited in the field of tissue engineering, particularly in an attempt to promote periodontal tissue regeneration (Hasegawa et al 2006).…”