Effects of organosolv and ammonia pretreatments on lignin properties and its inhibition for enzymatic hydrolysis

Yoo, Chang Geun; Li, Mi; Meng, Xianzhi; Pu, Yunqiao; Ragauskas, Arthur J.

doi:10.1039/c6gc03627a

Cited by 159 publications

(96 citation statements)

References 47 publications

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“…Lignin samples were acetylated prior to analysis according to method by Kumar [ 41 ]. Gel permeation chromatography was performed on an Agilent 1200 HPLC system (Agilent Technologies, Inc, Santa Clara, CA) with tetrahydrofuran (THF) as described in the previous study [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

A structured understanding of cellobiohydrolase I binding to poplar lignin fractions after dilute acid pretreatment

Yao

Yoo

Meng

et al. 2018

Biotechnol Biofuels

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BackgroundCellulase adsorption to lignin is considered a cost barrier for bioethanol production; however, its detailed association mechanism is still not fully understood. In this study, two natural poplar variants with high and low sugar release performance were selected as the low and high recalcitrant raw materials (named L and H, respectively). Three different lignin fractions were extracted using ethanol, followed by p-dioxane and then cellulase treatment from the dilute acid pretreated poplar solids (fraction 1, 2, and 3, respectively).ResultsEach lignin fraction had different physicochemical properties. Ethanol-extracted lignin had the lowest weight average molecular weight, while the molecular weights for the other two lignin fractions were similar. 31P NMR analysis revealed that lignin fraction with higher molecular weight contained more aliphatic hydroxyl groups and less phenolic hydroxyl groups. Semi-quantitative analysis by 2D HSQC NMR indicated that the lignin fractions isolated from the natural variants had different contents of syringyl (S), guaiacyl (G) and interunit linkages. Lignin extracted by ethanol contained the largest amount of S units, the smallest amounts of G and p-hydroxybenzoate (PB) subunits, while the contents of these lignin subunits in the other two lignin fractions were similar. The lignin fraction obtained after cellulase treatment was primarily comprised of β-O-4 linkages with small amounts of β-5 and β–β linkages. The binding strength of these three lignin fractions obtained by Langmuir equations were in the order of L1 > L3 > L2 for the low recalcitrance poplar and H1 > H2 > H3 for the high recalcitrance poplar.ConclusionsOverall, adsorption ability of lignin was correlated with the sugar release of poplar. Structural features of lignin were associated with its binding to CBH. For natural poplar variants, lignin fractions with lower molecular weight and polydispersity index (PDI) exhibited more CBH adsorption ability. Lignins with more phenolic hydroxyl groups had higher CBH binding strength. It was also found that lignin fractions with more condensed aromatics adsorbed more CBH likely attributed to stronger hydrophobic interactions.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1087-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

A structured understanding of cellobiohydrolase I binding to poplar lignin fractions after dilute acid pretreatment

Yao

Yoo

Meng

et al. 2018

Biotechnol Biofuels

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“…The organosolv pretreatment resulted in a signicant deconstruction of lignin by the cleavage of inter-unit linkages and condensation of S and G units in comparison with dilute acid and ammonia pretreatment. 19 For example, lignin were isolated, migrated and redistributed during organosolv pretreatment of Liriodendron tulipifera with the reformed droplets found on the surface of pretreated biomass. 20 Based on the above discussion, there is a gap in our understanding on the determining factors of lignin source, structure and composition on the electrochemical performances of the derived activated carbons.…”

Section: Introductionmentioning

confidence: 99%

Linking lignin source with structural and electrochemical properties of lignin-derived carbon materials

Zhang

Das

et al. 2018

RSC Adv.

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“…Both of these methodologies can significantly alter the structure of lignin. In general, these delignification strategies are implemented primarily to degrade, solubilize, and reduce the overall recalcitrance of the lignin‐hemicellulose‐cellulose matrix, providing greater access to cellulose fibers for subsequent biological processes, and resulting in a lignin byproduct with unique properties and potential for biofuel and material applications . For example, ethanol organosolv pretreatment (EOP) is a process that is being examined for industrial biorefineries .…”

Section: Ligninmentioning

confidence: 99%

Lignin‐derived electrochemical energy materials and systems

Jiang

Wang

et al. 2020

Biofuels Bioprod Bioref

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Electrode and electrolyte materials with higher performance, longer life, and lower cost need to be developed, given the substantial growing demand for advanced electrochemical energy systems. Lignin, the second most abundant natural polymer, has been successfully demonstrated to be a viable precursor or feedstock for the preparation of high-performance electrochemical energy materials and components such as electrodes, electrolyte additives, membrane separators, and binders. Moreover, techno-economic analyses indicate that it is possible to prepare cost-effective carbon structures from lignin at engineering scale, in contrast with current carbon products. These facts suggest that the scalable conversion of lignin into high-value energy materials will offer a promising pathway to not only promote the utilization and valorization of lignin but also boost the development of advanced electrochemical energy systems. This review examines cutting-edge renewable energy materials derived from various lignin compounds and Review: Lignin to energy materials X Wu et al.their applications in electrochemical energy systems with an emphasis on supercapacitors, rechargeable batteries, and fuel cells. Meanwhile, this review also aims to carve out the critical barriers for lignin-derived high-performance materials for energy applications, and to identify viable approaches for the synthesis of sustainable new energy materials.

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Effects of organosolv and ammonia pretreatments on lignin properties and its inhibition for enzymatic hydrolysis

Abstract: Effects of organosolv and ammonia pretreatments were understood by investigating the changes of lignin properties and its inhibition for enzymatic hydrolysis using diverse analysis methods.

Cited by 159 publications

References 47 publications

A structured understanding of cellobiohydrolase I binding to poplar lignin fractions after dilute acid pretreatment

A structured understanding of cellobiohydrolase I binding to poplar lignin fractions after dilute acid pretreatment

Linking lignin source with structural and electrochemical properties of lignin-derived carbon materials

Lignin‐derived electrochemical energy materials and systems

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