Effects of organism preparation in metallothionein and metal analysis in marine invertebrates for biomonitoring marine pollution

Oaten, J.F.P.; Hudson, Malcolm D.; Jensen, A.C.; Williams, I.D.

doi:10.1016/j.scitotenv.2015.03.003

Cited by 16 publications

(2 citation statements)

References 43 publications

(37 reference statements)

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“…A spectrophotometric method modified from [31] was used for quantifying MTLP in the soft tissues. The whole shell-free body was used for the analysis as it has been shown to be more reliable for long-term monitoring than dissected digestive glands [32]. The tissues of 3–6 snails were pooled into a sample (50.4 ± 0.2 mg wet weight; 12–18 samples per site) and homogenized with Buffer 1 (1:3) containing 0.5 M sucrose, 20 mM Tris-HCl buffer (pH 8.6), 0.006 mM leupeptin, 0.5 mM phenylmethylsulphonilfluoride (PMSF) and 0.01% β-mercaptoethanol.…”

Section: Methodsmentioning

confidence: 99%

Metal contamination in harbours impacts life-history traits and metallothionein levels in snails

et al. 2017

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Harbours with limited water exchange are hotspots of contaminant accumulation. Antifouling paints (AF) contribute to this accumulation by leaching biocides that may affect non-target species. In several leisure boat harbours and reference areas in the Baltic Sea, chronic exposure effects were evaluated using caging experiments with the snail Theodoxus fluviatilis. We analysed variations in ecologically relevant endpoints (mortality, growth and reproduction) in concert with variation in metallothionein-like proteins (MTLP) levels. The latter is a biomarker of exposure to metals, such as copper (Cu) and zinc (Zn), which are used in AF paints as active ingredient and stabilizer, respectively. In addition, environmental samples (water, sediment) were analysed for metal (Cu and Zn) and nutrient (total phosphorous and nitrogen) concentrations. All life-history endpoints were negatively affected by the exposure, with higher mortality, reduced growth and lower fecundity in the harbours compared to the reference sites. Metal concentrations were the key explanatory variables for all observed adverse effects, suggesting that metal-driven toxicity, which is likely to stem from AF paints, is a source of anthropogenic stress for biota in the harbours.

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Section: Methodsmentioning

confidence: 99%

Metal contamination in harbours impacts life-history traits and metallothionein levels in snails

et al. 2017

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“…Samples were carried out in January, April, August, and October, which are referred to as winter, spring, summer, and autumn. Samples were kept in storage at -20 °C before analysis, as advised by Oaten, et al [27].…”

Section: Sample Collectionmentioning

confidence: 99%

Metal Accumulation and Metallothionein Response in Fucus spiralis

Oaten¹,

Gibson²,

Hudson³

et al. 2016

Proceedings of the 2nd World Congress on New Technologies

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Interrelationships among trace metals and metallothionein in digestive glands and gills for field samples of Merceneria merceneria

Drira

Trefry

Trocine

2017

Environ Sci Pollut Res

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More widespread use of metallothionein (MT) as a biomarker for trace metal pollution continues to be partly dependent on obtaining reliable baseline concentrations and identifying increased induction of the enzyme with only modest increases in metal concentrations. In this study, new data on metals and MT levels in whole clams tissue, gills, and digestive glands from field samples and in sediments are presented. Concentrations of Cd, Cu, Fe, and Zn in depurated (24 h) clam samples of digestive glands, gills, and the whole clam Merceneria merceneria from the Indian River Lagoon, Florida, varied with location and showed moderate to strong correlations among Zn, Cu, and Fe. Concentrations of metallothionein (dry wt.) ranged from 34─270 μg/g in gills and 150-440 μg/g in digestive glands and showed moderate to strong correlations between organs and with metal concentrations in those organs. Observed trends support increased synthesis of metallothionein with only moderate increases in metal values and in response to statistically higher sediment metal concentrations.

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Effects of organism preparation in metallothionein and metal analysis in marine invertebrates for biomonitoring marine pollution

Cited by 16 publications

References 43 publications

Metal contamination in harbours impacts life-history traits and metallothionein levels in snails

Metal contamination in harbours impacts life-history traits and metallothionein levels in snails

Metal Accumulation and Metallothionein Response in Fucus spiralis

Interrelationships among trace metals and metallothionein in digestive glands and gills for field samples of Merceneria merceneria

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