Effects of Organic Amendments on Microbiota Associated with the Culex nigripalpus Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses

Duguma, Dagne; Hall, Michael W.; Smartt, Chelsea T.; Neufeld, Josh D.

doi:10.1128/msphere.00387-16

Cited by 21 publications

(27 citation statements)

References 69 publications

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“…However, little has been understood about the mechanisms of arbovirus replication in mosquitoes and the mechanisms of anti-viral immunity. It has been proposed that genetic diversity in wild mosquito populations or environments including microbial flora or virome would influence virus susceptibility [ 20 , 21 , 22 , 23 ]. As demonstrated in the results of recent metagenomic analyses, insects naturally harbor a variety of viruses regardless of their pathogenicity; however, the function of most of these viruses has not been determined [ 15 , 16 , 17 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses

Fujita

Kato

Kobayashi

et al. 2018

Heliyon

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In the growth kinetics analysis of flaviviruses in Aedes albopictus C6/36 cell lines obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank and the European Collection of Authenticated Cell Culture (ECACC), these two cells line showed different viral susceptibility for Zika virus (ZIKV), Dengue virus (DENV), and Japanese encephalitis virus (JEV). Next-generation sequencing (NGS) analysis revealed that the C6/36 JCRB strain was persistently infected with two viruses without showing any cytopathic effects. The complete sequence analysis demonstrated that the one virus was Menghai rhabdovirus (MERV), which has been found from Aedes albopictus mosquito. The other virus was a novel virus, designated as Shinobi tetravirus (SHTV). Interestingly, the viral susceptibility of these two strains was almost even for Sindbis virus and Getah virus. We cloned SHTV and MERV from JCRB C6/36 cell line and then re-infected them into another C6/36 cell line, resulting in the reproduction of persistent infection with each virus. ZIKV growth was suppressed in SHTV and/or MERV re-infected C6/36 cells also. To our knowledge, this is the first demonstration that persistent infection with rhabdovirus and/or permutotetravirus suppressed flavivirus replication in mosquito cells.

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Section: Discussionmentioning

confidence: 99%

Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses

Fujita

Kato

Kobayashi

et al. 2018

Heliyon

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“…Before genomic DNA extraction, each individual mosquito in the pooled sample was surface-sterilized by washing with distilled sterile water, and then submerged for 10 s in 70% ethanol, one min in 1% hypochlorite, and one min in PBS (phosphate-buffered saline) [ 25 ]; the washing steps were performed three times. This procedure is routinely used for sterilization of insect surfaces without considerable impact on the profiles of internal bacterial communities [ 26 ]. Genomic DNA was then extracted using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Zika virus infection modulates the bacterial diversity associated with Aedes aegypti as revealed by metagenomic analysis

et al. 2018

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Zika is a re-emerging infection that has been considered a major threat to global public health. Currently at least 100 countries are at risk of Zika virus (ZIKV) transmission. Aedes aegypti is the main mosquito vector in the Americas. This vector is exposed to, and interacts symbiotically with a variety of microorganisms in its environment, which may result in the formation of a lifetime association. Here, the unknown effect that ZIKV exerts on the dynamic bacterial community harbored by this mosquito vector was investigated using a metagenomic analysis of its microbiota. Groups of Ae. aegypti were experimentally fed on sugar, blood and blood mixed with ZIKV, and held for 3 to 7 days after blood meal and eggs development respectively. The infected groups were processed by qPCR to confirm the presence of ZIKV. All groups were analyzed by metagenomics (Illumina Hiseq Sequencing) and 16S rRNA amplicon sequences were obtained to create bacterial taxonomic profiles. A core microbiota and exclusive bacterial taxa were identified that incorporate 50.5% of the predicted reads from the dataset, with 40 Gram-negative and 9 Gram-positive families. To address how ZIKV invasion may disturb the ecological balance of the Ae. aegypti microbiota, a CCA analysis coupled with an explanatory matrix was performed to support the biological interpretation of shifts in bacterial signatures. Two f-OTUs appeared as potential biomarkers of ZIKV infection: Rhodobacteraceae and Desulfuromonadaceae. Coincidentally, both f-OTUs were exclusively present in the ZIKV-infected blood-fed and ZIKVinfected gravid groups. In conclusion, this study shows that bacterial symbionts act as biomarkers of the insect physiological states and how they respond as a community when ZIKV invades Ae. aegypti. Basic knowledge of local haematophagous vectors and their associated microbiota is relevant when addressing transmission of vector-borne infectious diseases in their regional surroundings.PLOS ONE | https://doi.org/10.1371/journal.pone

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“…While the bacteria reported above have been identified in other insects and some have known functions, other bacteria that we identified that have been reported from other insects require further investigation (i.e., Acinetobacter, Arcobacter, Bacillus, Burkholderia, Cloacibacterium, Delftia, Flavobacterium, Propionibacterium, and Sphingomonas;Srivastava and Rouatt 1963, Kikuchi et al 2005, Konig 2006, Xiang et al 2006, Killer et al 2009, Leroy et al 2011, Malhotra et al 2012, Morales-Jiménez et al 2012, Cakici et al 2014, Montagna et al 2014, Ceja-Navarro et al 2015, Meirelles et al 2016, Meriweather et al 2016, Segata et al 2016, Cheng et al 2017, Duguma et al 2017, Luna et al 2018. For example, Flavobacterium has been reported from pea aphids (Srivastava and Rouatt 1963), and while members of the order Flavobacteriales are reported to provision their insect hosts with nutrients (Wu et al 2006, Bennett et al 2014, Rosas-Pérez et al 2014, it is unknown whether this bacteria would have a similar role in SCA.…”

Section: Discussionmentioning

confidence: 80%

Differences in Microbiota Between Two Multilocus Lineages of the Sugarcane Aphid (Melanaphis sacchari) in the Continental United States

Holt

Styer

White

et al. 2020

Annals of the Entomological Society of America

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The sugarcane aphid (SCA), Melanaphis Sacchari (Zehntner) (Hemiptera: Aphididae), has been considered an invasive pest of sugarcane in the continental United States since 1977. Then, in 2013, SCA abruptly became a serious pest of U.S. sorghum and is now a sorghum pest in 22 states across the continental United States. Changes in insect-associated microbial community composition are known to influence host-plant range in aphids. In this study, we assessed whether changes in microbiota composition may explain the SCA outbreak in U.S. sorghum. We characterized the SCA bacterial microbiota on sugarcane and grain sorghum in four U.S. states, using a metabarcoding approach. In addition, we used taxon-specific polymerase chain reaction (PCR) primers to screen for bacteria commonly reported in aphid species. As anticipated, all SCA harbored the primary aphid endosymbiont Buchnera aphidicola, an obligate mutualistic bacterial symbiont. Interestingly, none of the secondary symbionts, facultative bacteria typically associated with aphids (e.g., Arsenophonus, Hamiltonella, Regiella) were present in either the metabarcoding data or PCR screens (with the exception of Rickettsiella and Serratia, which were detected by metabarcoding at low abundances <1%). However, our metabarcoding detected bacteria not previously identified in aphids (Arcobacter, Bifidobacterium, Citrobacter). Lastly, we found microbial host-associated differentiation in aphids that seems to correspond to genetically distinct aphid lineages that prefer to feed on grain sorghum (MLL-F) versus sugarcane (MLL-D).

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Effects of Organic Amendments on Microbiota Associated with the Culex nigripalpus Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses

Cited by 21 publications

References 69 publications

Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses

Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses

Zika virus infection modulates the bacterial diversity associated with Aedes aegypti as revealed by metagenomic analysis

Differences in Microbiota Between Two Multilocus Lineages of the Sugarcane Aphid (Melanaphis sacchari) in the Continental United States

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