Effects of oregano essential oil on in vitro ruminal fermentation, methane production, and ruminal microbial community

Zhou, Rui; Wu, Jianping; Xia, Lang; Liu, Lishan; Casper, D. P.; Wang, Cailian; Zhang, Liping; Wei, Shuo

doi:10.3168/jds.2019-16611

Cited by 80 publications

(79 citation statements)

References 80 publications

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“…However, further investigations are needed to evaluate the fate of the absorbed nutrients, especially calcium and N (259). Recently, Zhou et al (239) suggested that oregano EO (52 mg/L) in mature ruminants can modify ruminal fermentation and mitigate in vitro CH 4 production through mediating ruminal bacteria (Prevotella and Dialister). Some studies involving supplementation of EO in ruminants have shown contrary findings as the feeding of oregano EO did not reduce CH 4 yield together with no effect on animal performance and rumen fermentation (240,248).…”

Section: Effect Of Essential Oils On Rumen Methanogenesis and Fermentmentioning

confidence: 99%

Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction

et al. 2020

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Ruminants inhabit the consortia of gut microbes that play a critical functional role in their maintenance and nourishment by enabling them to use cellulosic and non-cellulosic feed material. These gut microbes perform major physiological activities, including digestion and metabolism of dietary components, to derive energy to meet major protein (65–85%) and energy (ca 80%) requirements of the host. Owing to their contribution to digestive physiology, rumen microbes are considered one of the crucial factors affecting feed conversion efficiency in ruminants. Any change in the rumen microbiome has an imperative effect on animal physiology. Ruminal microbes are fundamentally anaerobic and produce various compounds during rumen fermentation, which are directly used by the host or other microbes. Methane (CH 4 ) is produced by methanogens through utilizing metabolic hydrogen during rumen fermentation. Maximizing the flow of metabolic hydrogen in the rumen away from CH 4 and toward volatile fatty acids (VFA) would increase the efficiency of ruminant production and decrease its environmental impact. Understanding of microbial diversity and rumen dynamics is not only crucial for the optimization of host efficiency but also required to mediate emission of greenhouse gases (GHGs) from ruminants. There are various strategies to modulate the rumen microbiome, mainly including dietary interventions and the use of different feed additives. Phytogenic feed additives, mainly plant secondary compounds, have been shown to modulate rumen microflora and change rumen fermentation dynamics leading to enhanced animal performance. Many in vitro and in vivo studies aimed to evaluate the use of plant secondary metabolites in ruminants have been conducted using different plants or their extract or essential oils. This review specifically aims to provide insights into dietary interactions of rumen microbes and their subsequent consequences on rumen fermentation. Moreover, a comprehensive overview of the modulation of rumen microbiome by using phytogenic compounds (essential oils, saponins, and tannins) for manipulating rumen dynamics to mediate CH 4 emanation from livestock is presented. We have also discussed the pros and cons of each strategy along with future prospective of dietary modulation of rumen microbiome to improve the performance of ruminants while decreasing GHG emissions.

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Section: Effect Of Essential Oils On Rumen Methanogenesis and Fermentmentioning

confidence: 99%

Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction

et al. 2020

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“…Shen, Liu, Yu (39) reported that monensin and nisin supplementation decreased the absolute abundance of total bacteria and overall rumen fermentation (gas and VFA production) with a concomitant change in the major rumen microbiota. The supplementation of plant secondary metabolites such as thymol and oregano oil has recently shown to change the major microbiota with a concomitant reduction in feed digestion and rumen fermentation [38,40]. In the present study, endolysin LyJH307 did not affect the absolute abundance of total bacteria, ciliate protozoa (Table 3) and major microbiota from phylum to genus levels (having relative proportions > 1%) ( Figure 4).…”

Section: Discussionmentioning

confidence: 38%

“…In the present study, although the pH of both treatments was close to SARA conditions rather than acute acidosis (CON group, 5.57 vs. LyJH307 group, 5.69), it was assumed that a decrease in the S. bovis might be related to an increased ruminal pH in the LyJH307 supplemented group. Several agents to inhibit ruminal bacteria were evaluated in an in vitro system, and the ability to change the rumen microbiota and rumen fermentation has been shown [37][38][39]. Shen, Liu, Yu (39) reported that monensin and nisin supplementation decreased the absolute abundance of total bacteria and overall rumen fermentation (gas and VFA production) with a concomitant change in the major rumen microbiota.…”

Section: Discussionmentioning

confidence: 99%

Specific inhibition of Streptococcus bovis by endolysin LyJH307 supplementation shifts the rumen microbiota and metabolic pathways related to carbohydrate metabolism

Kim

Park

Kwon³

et al. 2021

Preprint

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Background: Ruminal acidosis negatively affects the animal production and performances of high-performance cattle by abnormal balancing of the rumen microbiota, which caused significant economic damage to farmers. Endolysins, the bacteriophage-originated peptidoglycan hydrolases, are a promising replacement of antibiotics due to their immediate lytic activity and no evidence of antibiotic resistance. The objectives of this study were to investigate the lytic activity of Streptococcus bovis specific endolysin LyJH307 against S. bovis and explore changes in rumen fermentation and microbiota in an in vitro system. Treatments consisted of corn grain with protein elution buffer and corn grain with endolysin LyJH307 (LyJH307, 0.2% of dietary dry matter). The rumen bacterial community was analyzed using 16S rRNA amplicon sequencing, followed by the prediction of microbial functional features by using PICRUSt2.Results: Endolysin LyJH307 supplementation at 6 h incubation time markedly decreased the absolute abundance of S. bovis (approximately 70%) and increased ruminal pH at the end of the incubation. There were no significant changes in gas production, in vitro dry matter degradability, and total volatile fatty acid production. The acetate proportion was significantly increased after LyJH307 addition, whereas propionate was decreased, thereby inducing an increase of acetate to propionate ratio in LyJH307. LyJH307 supplementation increased D-lactate without any change in L-lactate concentration. Among the alpha diversity indices, there were no significant differences in Shannon’s index, Simpson’s index, Chao1 estimates, and evenness. Based on Bray-Curtis dissimilarity matrices, the LyJH307 affected the overall shift in ruminal microbiota. Endolysin LyJH307 supplementation induced an increase of 11 genera containing Lachnoclostridium, WCHB1-41, unclassified genus Selenomonadaceae, Paraprevotella, vadinBE97, Ruminococcus gauvreauii group, Lactobacillus, Anaerorhabdus furcosa group, Victivallaceae, Desulfuromonadaceae, and Sediminispirochaeta. The predicted functional features represented by the Kyoto Encyclopedia of Genes and Genomes pathways were changed by LyJH307 toward a decrease of carbohydrate metabolism.Conclusions: Endolysin LyJH307 caused a reduction of S. bovis and an increase of ruminal pH with concomitant shifts in minor rumen microbiota and its metabolic pathways related to carbohydrate metabolism. This study provides the first insight into the availability of endolysin as a specific modulator for rumen and shows the possibility of endolysin degradation by rumen microbiota.

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“…Moreover, ORE-SIL combination depressed the DMD24 of the TMR while an opposite effect was observed on the NDFD24 of the same substrate. Partially in contrast with these results, Zhou et al (2020) reported an increase of the in vitro digestibility of both DM, NDF and ADF of a maize-silage based TMR with adding oregano oil (especially at 52 mg/L and 91 mg/L) supported by lactic acid, cobalt carbonate and clinoptiolite. Whereas, no effects on both DMD24 and NDFD24 were observed by Benchaar (2020) on TMR adding both carvacrol and oregano oil supported by SIL (50 mg/Kg).…”

Section: Discussionmentioning

confidence: 59%

Effects of the combination between selected phytochemicals and the carriers silica and Tween 80 on dry matter and neutral detergent fibre digestibility of common feeds

Simoni

Temmar

Bignamini

et al. 2020

Italian Journal of Animal Science

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Effects of oregano essential oil on in vitro ruminal fermentation, methane production, and ruminal microbial community

Cited by 80 publications

References 80 publications

Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction

Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction

Specific inhibition of Streptococcus bovis by endolysin LyJH307 supplementation shifts the rumen microbiota and metabolic pathways related to carbohydrate metabolism

Effects of the combination between selected phytochemicals and the carriers silica and Tween 80 on dry matter and neutral detergent fibre digestibility of common feeds

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