Effects of oocyte quality, semen donor and embryo co-culture system on the efficiency of blastocyst production in goats

Effects of oocyte quality and semen donor on the efficiency of <i>in vitro</i> embryo production in cattle

“…This implies that the BCB test is a useful tool for selection of more developmentally competent oocytes. These results are in agreement with similar studies in cattle (Pujol et al, 2004;Alm et al, 2005;Bhojwani et al, 2007), goat (Rodriguez-González et al, 2003;Kątska-Książkiewicz et al, 2007) and pig (Wongsrikeao et al, 2006). Selection of excellent quality oocytes is highly desirable for procedures involving extensive manipulations such as pronuclear microinjections, intracytoplasmic sperm injection, and nuclear transplantation, which requires high developmental competence after embryo transfer.…”

“…These bull-to-bull variations in the outcome of in vitro embryo production frequently lead to selection of bulls used for IVF. Also, in several other mammalian species, male-to-male variations in the outcome of in vitro embryo production have been reported (Cognie, 1999;Kątska-Książkiewicz et al, 2007). For these reasons semen from a male of proven fertility is commonly used for IVF purposes.…”

“…Therefore, non-invasive markers of oocyte quality that allow for accurate and fast selection prior to IVM have become of prime importance. It has been demonstrated that brilliant cresyl blue (BCB) can be used for the selection of competent oocytes in pig, goat and cattle (Rodriguez-González et al, 2003;Pujol et al, 2004;Alm et al, 2005;Wongsrikeao et al, 2006;Bhojwani et al, 2007;Kątska-Książkiewicz et al, 2007). Although it was previously shown that BCB staining is a useful tool for selecting more competent oocytes, the test is still not routinely included in laboratory selection protocols.…”

Effect of embryonic fibroblast cell co-culture on development of mouse embryos following exposure to visible light

“…Even if this argument is correct, it should be noted that in suboptimal conditions, which are most often present in ART laboratories, coculture systems might eliminate the harmful effects of environmental factors on embryo development. Many studies have reported the beneficial effects of co-cultures on embryo development in vitro in humans [1,32,33]; domestic animals [34,35] and rodents [19,26,27]. However, it is not fully elucidated why co-culture of embryos with somatic cells improves embryo development.…”

The effects of <italic>in vitro</italic> maturation duration on maturation rate and intracytoplasmic sperm injection-derived embryonic development in goats

“…LOPU is a minimally invasive technique and offers faster post-operative recovery than standard laparotomy (Tibary et al, 2005); it is also reported to be an efficient method of providing oocytes (Abdullah et al, 2008;Kong, 2010). Katska-Ksiazkiewicz et al (2007) reported that oocyte quality might influence the success of in vitro production of goat embryos. Several researchers obtained a better maturation rate when they cultured oocytes with at least four complete layers (Jimenez-Macedo et al, 2007), two or more complete layers (Rho et al, 2001) or one or two complete layers (Wang et al, 2003) of cumulus cells.…”