Effects of oocyte quality on development and transcriptional activity in early bovine embryos

Bilodeau‐Goeseels, Sylvie; Panich, Paul L.

doi:10.1016/s0378-4320(01)00188-9

Cited by 84 publications

(65 citation statements)

References 43 publications

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“…Optimal granulosa and cumulus cell functioning are indispensable for oocyte maturation because these cells are responsible for endocrine and paracrine signaling (Bilodeau-Goeseels and Panich, 2002;Tanghe et al, 2002). Therefore, it is most likely that the toxic effect of NEFA on oocyte quality is partly an indirect effect.…”

Section: Introductionmentioning

confidence: 99%

The consequences of metabolic changes in high-yielding dairy cows on oocyte and embryo quality

Leroy

Soom

Opsomer

et al. 2008

Animal

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Unsatisfactory reproductive performance in dairy cows, such as reduced conception rates, in addition to an increased incidence of early embryonic mortality, is reported worldwide and has been associated with a period of negative energy balance (NEB) early post partum. Typically, NEB is associated with biochemical changes such as high non-esterified fatty acid (NEFA), high b-hydroxybutyrate (b-OHB) and low glucose concentrations. The concentrations of these and other metabolites in the follicular fluid (FF) of high-yielding dairy cows during NEB were determined and extensively analyzed, and then were replicated in in vitro maturation models to investigate their effect on oocyte quality. The results showed that typical metabolic changes during NEB are well reflected in the FF of the dominant follicle. However, the oocyte seems to be relatively isolated from extremely elevated NEFA or very low glucose concentrations in the blood. Nevertheless, the in vitro maturation models revealed that NEB-associated high NEFA and low glucose levels in the FF are indeed toxic to the oocyte, resulting in deficient oocyte maturation and developmental competence. Induced apoptosis and necrosis in the cumulus cells was particularly obvious. Furthermore, maturation in saturated free fatty acid-rich media had a carry-over effect on embryo quality, leading to reduced cryotolerance of day 7 embryos. Only b-OHB showed an additive toxic effect in moderately hypoglycemic maturation conditions. These in vitro maturation models, based on in vivo observations, suggest that a period of NEB may hamper the fertility of high-yielding dairy cows through increased NEFA and decreased glucose concentrations in the FF directly affecting oocyte quality. In addition to oocyte quality, these results also demonstrate that embryo quality is reduced following an NEB episode. This important observation may be linked to the typical diet provided to stimulate milk yield, or to physiological adaptations sustaining the high milk production. Research into this phenomenon is ongoing.

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Section: Introductionmentioning

confidence: 99%

The consequences of metabolic changes in high-yielding dairy cows on oocyte and embryo quality

Leroy

Soom

Opsomer

et al. 2008

Animal

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“…Follicular oocytes obtained from slaughtered animals have been found highly variable in their developmental competence after in vitro maturation (IVM; Gordon & Lu 1990, Bilodeau-Goeseels & Panich 2002. Accurate, fast, and non-invasive markers of oocyte quality are therefore in urgent need for oocyte selection prior to IVM.…”

Section: Introductionmentioning

confidence: 99%

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Han¹,

Lan²,

Wu³

et al. 2006

Reproduction

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Interactive effects of granulosa cell apoptosis, follicle size, cumulus-oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system Zheng-Bin Han, Guo-Cheng Lan, Yan-Guang Wu, Dong Han, Wei-Guo Feng, Jun-Zuo Wang and Jing-He Tan AbstractUsing a well-in-drop (WID) oocyte/embryo culture system that allows identification of follicular origin, we have investigated the effects of granulosa cells (GCs) apoptosis, follicle size, cumulus-oocyte complexes (COCs) morphology, and cumulus expansion on the developmental competence of goat oocytes matured and cultured individually following parthenogenetic activation. The WID system supported oocyte maturation and embryo development to a level similar to the conventional group system. The majority of goat oocytes acquired competence for development up to the 8-16 cell stage in follicles larger than 2 mm, but did not gain the ability to form morula/blastocyst (M/Bs) until follicles larger than 3 mm in diameter. The extent of atresia affected M/Bs formation. This effect varied according to the follicle size. Cumulus expansion increased with follicle size and decreased with increasing incidence of GCs apoptosis. Oocyte developmental potential was also correlated with cumulus expansion. Regardless of the degree of follicle atresia, 73-84% of the floating cells in the follicular fluid (FF) underwent apoptosis. Correlation between floating cell density in FF and oocyte developmental potency suggests the possibility to use the floating cell density as a simple and non-invasive marker for oocyte quality. It is concluded that the developmental potential of an oocyte is determined by multifactor interactions, and multiple factors must be considered together to accurately predict the quality of an oocyte. Reproduction (2006) 132 749-758Introduction A number of studies have shown that in vitro development of pre-implantation stage mammalian embryos is better when cultured in groups (group culture) than cultured singly in microdrops (microdrop individual culture, MIC) (Gardner et al. 1994, Kato & Tsunoda 1994, Keefer et al. 1994, Moessner & Dodson 1995. However, apart from the large-scale production of embryos for experimental purposes, most commercial and human embryo culture systems require culturing individually or in small groups. The reason for this need is the small number of oocytes available (ovum pick-up (OPU) technology, single slaughtered valuable oocyte donors and human IVF) or the required identification after culture. Besides, sometimes zona-free eggs must be cultured in isolation from each other to prevent conglomeration. Therefore, the establishment of an individual culture system that is as efficient as group culture has become of prime importance. Furthermore, although there are quite a few reports on individual culture of embryos, studies on individual culture of oocytes during maturation are few and with conflicting results (Carolan et al. 1996, Hagemann et al. 1998, Jewgenow et al. 19...

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“…The correlations between follicle atresia and oocyte developmental competence have been well demonstrated in the bovine model, in which healthy follicles were highly correlated with oocytes exhibiting greater developmental potential whilst atretic follicles contained oocytes with lesser development capacity (Bilodeau-Goeseels and Panich, 2002;Boni et al, 2002). Similarly, our recent studies found that the health status of rhesus monkey follicles, as assessed morphologically by the degree of cumulus investment, had a significant influence on the in vitro maturation of oocytes (Zheng and Ji, unpublished data).…”

Section: Effect Of Follicle Status On Oocyte Ivm In Rhesus Monkeysmentioning

confidence: 57%

Effects of in vitro maturation of monkey oocytes on their developmental capacity

Zheng

2007

Animal Reproduction Science

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The study of in vitro maturation (IVM) of rhesus monkey oocytes has important implications for biomedical research and human infertility treatment. In vitro-matured rhesus monkey oocytes show much less developmental potential than IVM oocytes of other species. Since about 1980 when rhesus monkey IVM, in vitro fertilization (IVF) and in vitro embryo culture (IVC) systems were established, numerous efforts have been made to improve the developmental competence of oocytes and to understand the mechanisms regulating oocyte maturation. This review describes recent progress in this area, particularly the effects of factors such as steroid hormones, energy substrates, amino acids, ovarian follicle status, maternal age and breeding season on the developmental competence, gene expression patterns and genome integrity of rhesus IVM oocytes.

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Effects of oocyte quality on development and transcriptional activity in early bovine embryos

Cited by 84 publications

References 43 publications

The consequences of metabolic changes in high-yielding dairy cows on oocyte and embryo quality

The consequences of metabolic changes in high-yielding dairy cows on oocyte and embryo quality

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Effects of in vitro maturation of monkey oocytes on their developmental capacity

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