Effects of once- versus twice-weekly transvaginal follicular aspiration on bovine oocyte recovery and embryo development

Gibbons, J.; Beal, W. E.; Krisher, Rebecca L.; Faber, E.G.; Pearson, R.E.; Gwazdauskas, F.C.

doi:10.1016/0093-691x(94)90679-d

Cited by 87 publications

(70 citation statements)

References 23 publications

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“…This means that more transferable embryos per week can be generated from twice-weekly aspiration than from once-weekly aspiration. The present results using the same cows for different treatments are consistent with the research by Gibbons et al [13], in which different groups of cows were used for each treatment. Bungartz et al [14] reported that cows did not show estrus under the twiceweekly aspiration regimen but they showed a normal estrus cycle under the once-weekly aspiration regimen.…”

Section: Discussionsupporting

confidence: 82%

“…Once a week versus twice a week transvaginal follicular aspiration frequencies with or without FSH stimulation [13,14] were compared to determine if there were differences in oocyte recovery and subsequent embryo production in vitro. In addition, the size and the number of follicles at the time of aspiration were examined to compare the growth of ovarian follicles among 4 different treatments.…”

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confidence: 99%

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Evaluation of Once- Versus Twice-Weekly Transvaginal Ultrasound-Guided Follicular Oocyte Aspiration With or Without FSH Stimulation from the Same Cows.

Goto

Tanimoto

Fujii

et al. 1995

Journal of Reproduction and Development

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Section: Discussionsupporting

confidence: 82%

mentioning

confidence: 99%

Evaluation of Once- Versus Twice-Weekly Transvaginal Ultrasound-Guided Follicular Oocyte Aspiration With or Without FSH Stimulation from the Same Cows.

Goto

Tanimoto

Fujii

et al. 1995

Journal of Reproduction and Development

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“…2). Alguns autores sugeriram que o FSH beneficia o desenvolvimento embrionário por sincronizar a população folicular, adiantando o desenvolvimento dos folículos e iniciando a maturação in vivo do oócitos, o que levaria a um aumento da competência de desenvolvimento oocitária antes da recuperação dos mesmos (Gibbons et al, 1994). Além disso, o uso do FSH pode alterar o ambiente folicular ao redor do oócito, influenciando direta ou indiretamente a qualidade do oócito (Roth et al, 2002).…”

Section: Resultsunclassified

Protocolos de produção in vitro de embriões na raça Gir

Ramos

Ferreira

Sá

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliaram-se os efeitos de dois protocolos de punção folicular na quantidade/qualidade dos oócitos e na produção in vitro de embriões, em vacas da raça Gir, não-lactantes. O ciclo estral foi sincronizado com cloprostenol e ao longo do experimento os animais receberam implantes auriculares de norgestomet, renovados a cada 14 dias. Os animais foram submetidos aos protocolos I (sem estimulação hormonal, com punção folicular duas vezes/semana) e II (pré-tratamento com 250 UI de FSH em doses decrescentes, três dias antes da punção folicular). Os oócitos recuperados foram levados ao laboratório em meio TALP-Hepes e submetidos à maturação. Na fecundação in vitro utilizou-se sêmen de touro Gir, previamente capacitado. Após 22 horas de fecundação, os prováveis zigotos foram co-cultivados com células da granulosa em CR2aa acrescido de 10% de soro fetal bovino. A taxa de clivagem foi avaliada 72 horas pós-fecundação e a de blastocisto 192 horas pós-fecundação. O número total de folículos foi maior (P<0,05) no protocolo II, assim como o número de folículos grandes e médios (P<0,05) e o diâmetro do maior folículo (P<0,05), sendo o número de folículos pequenos (P<0,05) menor. O total de oócitos recuperados por sessão não foi diferente entre os protocolos I e II (P>0,05). O número de oócitos de grau I e a taxa de clivagem foram maiores (P<0,05) e o número de degenerados menor protocolo II. A pré-estimulação ovariana com FSH pode melhorar a qualidade e a taxa de clivagem dos oócitos recuperados por punção folicular em animais Gir.Palavras-chave: bovino, Gir, embrião in vitro, punção folicular, FSH ABSTRACT The effect of two ovum pick-up protocols on amount/quality of oocytes retrived and on in vitro embryo production of Gyr cows was evaluated in non-lactating cycling cows with good body and reproductive conditions. Estrous cycles were synchronized with cloprostenol. During the experiment, animals received norgestomet ear implants, replaced every 14 days. Animals were submitted to protocol I (without hormonal estimulation) and protocol II (pre-stimulation with 250 IU of FSH three days before aspiration). Recovered oocytes were transported in Talp-

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“…In EXP 3, FSH treatment on 70 d of pregnancy, a phase during which the dominant follicle exerts regressive effects, led to synchronized growth of a group of subordinate smaller follicles and the 5-day-interval repeated FSH treatment and aspiration schedule was beneficial for suppressing the emergence of dominant follicles and resulted in higher yields of viable oocytes. Otherwise, the aspiration interval seemed to be more important than FSH treatment for the recovery of viable oocytes from donors [6,9,13,36,37,40,42]. Furthermore, Fry et al [8] reported that vaccination against inhibin on follicular development increased the numbers of aspirated follicles, but did not improve aspiration results.…”

Section: Discussionmentioning

confidence: 99%

“…Using ultrasound-guided technology, this procedure proved to be an effective, repeatable and safe method for viable oocyte recovery from valuable pregnant donors. However, Gibbons et al [9] reported that aspiration frequency may have elevated endogenous FSH levels. An increase of endogenous FSH due to aspiration frequency probably overshadowed any effects from exogenous FSH.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound-Guided Follicle Aspiration and IVF in Dairy Cows Treated with FSH after Removal of the Estrous Cycle.

Ooe

Rajamahendran

Boediono

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Recently, transvaginal ultrasound-guided follicle aspiration technology has been found to be of great value for in vitro fertilization (IVF) programs, even though the oocyte recovery rate and cleavage rate of transferrable embryos were low. In this study, we investigated the effect of the removal of the dominant follicle at different stages of the estrous cycle on the ovarian response of donor cows. Four experiments (EXPs) were devised. In EXP 1, 3 cows received 20 mg FSH on Day 1, ovulation occurred on Day 0, and on Day 3 follicles were aspirated. In EXP 2, the dominant follicle of the first wave was removed on Day 6 from 3 cows which received 20 mg FSH on Day 7 and on Day 9 follicles were aspirated. In EXP 3, 2 pregnant cows received 20 mg FSH on 70 d of pregnancy and 48 hr later follicles were aspirated a total of 5 times at 5-day intervals. In EXP 4, after ovulation on Day 0, 9 cows received 20 mg FSH on Days 8 to 14 of the estrous cycle and 48 hr after the last injection, follicles were aspirated once. The respective mean ± SD numbers of aspirated follicles and recovered oocytes were higher (p<0.01) in EXP 1 (13.4 ± 1.7 and 8.7 ± 2.3), EXP 2 (12.1 ± 1.4 and 7.7 ± 1.7) and EXP 3 (10.7 ± 2.1 and 7.0 ± 2.2) than in EXP 4 (5.8 ± 2.3 and 3.1 ± 1.6). The oocyte recovery rates were higher (p<0.05) in EXP 1, EXP 2 and EXP 3 than in EXP 4. Similarly, the respective numbers of viable oocytes and cleavage rates were higher in EXP 1, EXP 2 and EXP 3 (6.0 ± 1.3, 5.0 ± 1.1 and 4.6 ± 1.5 viable oocytes (p<0.01); 66, 73 and 65% cleavage rates (p<0.05)) than in EXP 4 (2.4 ± 1.1; 46%). The numbers of morulae and blastocysts were higher (p<0.05) in EXP 1, EXP 2 and EXP 3 than in EXP 4. In conclusion 1) removal of the dominant follicles from lactating and pregnant cows enabled viable oocytes to be recovered constantly and repeatedly by aspiration at different reproductive stages, and that viable blastocysts can be produced after IVF. 2) The presence or absence of a dominant follicle significantly affects the ovarian responses to FSH treatment. 3) This ultrasound-guided procedure proved to be an effective, repeatable and safe method for viable oocyte recovery from valuable pregnant donors. -KEY WORDS: cattle, dominant follicle removal, follicle aspiration, oocyte, ultrasound.

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Effects of once- versus twice-weekly transvaginal follicular aspiration on bovine oocyte recovery and embryo development

Cited by 87 publications

References 23 publications

Evaluation of Once- Versus Twice-Weekly Transvaginal Ultrasound-Guided Follicular Oocyte Aspiration With or Without FSH Stimulation from the Same Cows.

Evaluation of Once- Versus Twice-Weekly Transvaginal Ultrasound-Guided Follicular Oocyte Aspiration With or Without FSH Stimulation from the Same Cows.

Protocolos de produção in vitro de embriões na raça Gir

Ultrasound-Guided Follicle Aspiration and IVF in Dairy Cows Treated with FSH after Removal of the Estrous Cycle.

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