Effects of on genes expression pattern in small bowel mucosa

Di, Simona; Hong, Tao; Grillo, Antonino; Elia, Chiara; Gasbarrini, Giovanni; Sepulveda, Antonia R.; Gasbarrini, Antonio

doi:10.1016/j.dld.2004.12.008

Cited by 118 publications

(107 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our present findings are supported by another clinical study carried out in healthy adults showing that L. rhamnosus GG treatment leads to decreased TNF-α production in PBMC [31] . In addition, when the cytokine expression pattern in the small bowel mucosa was studied, it was found that L. rhamnosus GG induced the expression of genes involved in immune response and inflammation (TGF-beta and TNF family members, cytokines, nitric oxide synthase 1, defensin alpha 1) [32] . Schultz and coworkers [31] observed a decreased IL-6 and IFN-γ and an increased IL-10 and IL-4 production in PBMC obtained from L. rhamnosus GG treated individuals.…”

Section: Discussionmentioning

confidence: 99%

Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults

Kekkonen

Lummela²,

Karjalainen³

et al. 2008

WJG

184

152

View full text Add to dashboard Cite

freudenreichii ssp. shermanii JS (PJS) or a placebo drink for 3 wk. Venous blood and saliva samples were taken at baseline and on d 1, 7 and 21. Fecal samples were collected at baseline and at the end of intervention. RESULTS:The serum hsCRP expressed as the median AUC0-21 (minus baseline) was 0.018 mg/L in the placebo group, -0.240 mg/L in the LGG group, 0.090 mg/L in the Bb12 group and -0.085 mg/L in the PJS group (P = 0.014). In vitro production of TNF-α from in vitro cultured peripheral blood mononuclear cells (PBMC) was significantly lower in subjects receiving LGG vs placebo.IL-2 production from PBMC in the Bb12 group was significantly lower compared with the other groups. CONCLUSION:In conclusion, probiotic bacteria have strain-specific anti-inflammatory effects in healthy adults.

show abstract

Section: Discussionmentioning

confidence: 99%

Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults

Kekkonen

Lummela²,

Karjalainen³

et al. 2008

WJG

184

152

View full text Add to dashboard Cite

show abstract

“…A specific probiotic has been found to modulate the expression of genes involved in cell growth and differentiation in the intestinal mucosa of human volunteers [27] . Probiotics have also been successfully applied in patients with IBD [28,29] or diverticulitis [30] . Several studies indicate that aberrancies in faecal microbiota are present in different diseases, and our results strengthen those studies with colonic mucosa data.…”

Section: Discussionmentioning

confidence: 99%

Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis and inflammatory bowel disease

Gueimonde

Ouwehand²,

Huhtinen³

et al. 2007

WJG

106

View full text Add to dashboard Cite

AIM:To characterize the bifidobacterial microbiota of the colonic mucosa in patients with colon cancer, inflammatory bowel disease or diverticulitis. METHODS:A sample of the distal colonic mucosa was taken during surgery from a total of 34 patients, twenty-one with diagnosed colorectal cancer, nine with diverticulitis and four with inflammatory bowel disease, requiring surgery for their condition. Bacterial DNA was extracted from the resected mucosal samples and bifidobacterial mucosa-associated microbiota was qualitatively and quantitatively determined by means of qualitative and quantitative PCR. RESULTS:Bifidobacteria were found in 100% of the samples from patients with diverticulitis or IBD and a 76% of those suffering colon cancer. The species B. longum and B. bifidum were the most widely found, followed by B. animalis , B. catenulatum and B. adolescentis . B. breve , B. dentium and B. angulatum were not detected in any sample. A significantly higher occurrence of B. longum was observed in patients with diverticulitis than in those with colon cancer or IBD (100%, 62% and 75%, respectively, P < 0.05). Similar results were obtained for B. animalis (56%, 0% and 25%, P < 0.05), while B. adolescentis was only found in the mucosa from patients with colon cancer (5 out of 21, 24%). At the quantitative level, patients with colon cancer or IBD showed lower counts of total Bifidobacterium (4.94 and 5.91 vs 6.96 log Cells/sample, respectively, P < 0.05) and of the species B. longum (4.05 and 4.79 vs 6.76, P < 0.05) than those with diverticulitis. CONCLUSION:Aberrancies in mucosa associated microbiota are present in different intestinal diseases. This may indicate a role of the microbiota in the pathogenesis of these diseases.

show abstract

“…Important information has also been obtained in studies that used human biopsies after probiotic administration for transcriptomic analyses. The administration of Lactobacillus rhamnosus GG affected mainly the expression of genes involved in the immune and inflammatory response, as well as genes related to apoptosis, cell growth and differentiation, signaling and adhesion (di Caro et al 2005). In a different study, volunteers received Lactobacillus plantarum WCFS1 through an intraduodenal cannula from where biopsies were taken after 6 h. Authors found differences in the modulation of NF-jB-related pathways (Troost et al 2008;van Baarlen et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

et al. 2012

View full text Add to dashboard Cite

Significant health benefits have been demonstrated for certain probiotic strains through intervention studies; however, there is a shortage of experimental evidence relative to the mechanisms of action. Here, noninvasive experimental procedure based on a colon organ culture system has been used that, in contrast to most experimental in vitro models reported, can preserve natural immunohistochemical features of the human mucosa. This system has been used to test whether commensal lactobacilli (Lactobacillus paracasei BL23, Lactobacillus plantarum 299v and L. plantarum 299v (A -)) were able to hinder inflammation-like signals induced by phorbol 12-myristate 13-acetate (PMA)/ionomycin (IO). Whole genome microarrays have been applied to analyze expression differences, from which mRNA markers could be inferred to monitor the effect of putative probiotic strains under such conditions. Regarding the gene expression, PMA/IO treatment induced not only interleukin (IL)-2 and interferon gamma (IFN-c), as expected, but also other relevant genes related to immune response and inflammation, such as IL-17A, chemokine (C-X-C motif) ligand (CXCL) 9 and CXCL11. The ex vivo culturing did not modify the pattern of expression of those genes or others related to inflammation. Interestingly, this study demonstrated that lactobacilli downregulated those genes and triggered a global change of the transcriptional profile that indicated a clear homeostasis restoring effect and a decrease in signals produced by activated T cells.

show abstract

Effects of on genes expression pattern in small bowel mucosa

Cited by 118 publications

References 38 publications

Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults

Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults

Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis and inflammatory bowel disease

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

Contact Info

Product

Resources

About