Effects of Oleamide on Choline Acetyltransferase and Cognitive Activities

Effects of Green Tea Polyphenol on Cognitive and Acetylcholinesterase Activities

“…8) The procedure was basically the same as that described previously. 7) Each mouse, naive to the maze, was placed at the end of one arm and allowed to move freely through the maze during an 8-min session. The series of arm entries was recorded visually, and alternation was defined as successive entries into the three arms nonoverlapping triplet sets.…”

Daidzein Activates Choline Acetyltransferase from MC-IXC Cells and Improves Drug-Induced Amnesia

“…MC-IXC cells were cultured and maintained according to the previously described methods. 6) The cells, which were obtained from a human neuroblastoma, were grown in Minimum Essential Medium (MEM) containing 15% fetal bovine serum (FBS). The MC-IXC cells were grown in the presence of penicillin (100 U/ml) and streptomycin (0.1 mg/ml) at 37 C, in an atmosphere containing 5% CO 2 .…”

“…The ChAT activity in the MC-IXC cells was measured by a modification of the method described by Fonnum. 7) In the preparation of the enzyme source, the MC-IXC cells were homogenized using a Glass-Col homogenizer (Terre Haute, IN, U.S.A.) with 5 volumes of homogenizing buffer [20 mM Tris-HCl (pH 7.2), containing 150 mM NaCl, 10 mM MgCl 2 , and 0.5% Triton X-100], 6) and centrifuged for 30 min at 10;000 g. The resulting supernatant was then utilized as an enzyme source. All extraction steps were conducted at 4 C. Protein concentrations were measured with a BCA kit (Bicinchoninic acid; Sigma, St. Louis, MO, U.S.A.), using bovine serum albumin (BSA) as the protein standard.…”

“…6,10) The Y-maze task was conducted after 4 weeks of daidzein administration. The maze used in this study was constructed of black-painted wood, and each of the arms of the maze was 25 cm long, 14 cm high, and 5 cm wide.…”

Apoptosis Induction Effect of Three Jujube Cultivars in HepG2 and Jurkat Cell Lines

“…It has long been recognized as an edible fruit [2]. In addition, various parts of jujubes were reported to be useful for treatment of many symptoms and diseases, for example; diabetes (96% ethanolic extract of Z. jujuba leaves) [3], diarrhea (methanolic extract of Z. mauritiana roots) [4], inhibition of hepatic lipid peroxidation Manuscript (aqueous extract of Z. mauritania leaves) [5], anti-fungal activity (ethanolic extract of Z. jujuba stone) [6] enhancing choline acetyltransferase associated with Alzheimer's disease (methanolic extract of Z. jujuba fruits) [7], anxiolytic effect (methanolic extract of Z. jujuba seed) [8], and enhanced permeability in cell culture monolayer (aqueous extract of Z. jujuba seed) [9]. The active compounds found in jujube were triterpeonic acid (roots) [10], 6'''-feruloylspinosin, 6'''-sinapoylspinosin, jujuboside A, B (seeds) [11], linoleic, oleic and stearic acids (seeds) [12].…”