Effects of olanexidine gluconate on preoperative skin preparation: an experimental study in cynomolgus monkeys

Abstract

PurposeTo determine the bactericidal efficacy of a new topical antiseptic for preoperative skin preparation, olanexidine gluconate (development code: OPB-2045G), against transient or resident bacterial flora on the skin of cynomolgus monkeys.MethodologyAfter measuring baseline bacterial counts on test sites marked on the abdomens, we applied olanexidine, chlorhexidine or povidone–iodine. After 10 min (fast-acting effect) and 6 h (long-lasting effect), bacterial counts were measured again and log10 reductions were calculated. In addition, we determined the bactericidal effects on the skin contaminated with blood before or after applying the antiseptics.ResultsIn the non-blood-contaminated condition, the mean log10 reductions of olanexidine at doses of 1–2 % were significantly higher than those of saline (negative control), but did not significantly differ from those of 0.5 % chlorhexidine and 10 % povidone–iodine at either time point. But olanexidine was significantly more effective at both time points than chlorhexidine and povidone–iodine when applied after the site was contaminated with blood. Olanexidine was also significantly more effective than chlorhexidine and as effective as or more effective than povidone-iodine at both time points when skin was contaminated with blood after the antiseptics were applied.ConclusionThe bactericidal effects of olanexidine were comparable to those of commercial antiseptics such as chlorhexidine and povidone–iodine in non-blood-contaminated conditions. More importantly, the effect of olanexidine was hardly affected by blood unlike commercial antiseptics. Thus, it is considered that olanexidine has a favourable property for skin preparation in various types of surgical treatments.

Evaluation of <i>in Vitro</i> Bactericidal Activity of 1.5% Olanexidine Gluconate, a Novel Biguanide Antiseptic Agent

“…These data were consistent with previous reports on the activity of chlorhexidine gluconate. 17,18) Our data showed that olanexidine gluconate has strong bactericidal activity and broad antibacterial and antifungal spectra. Hagi et al reported that bactericidal activity of olanexidine gluconate against various bacterial species was compared to CHG and PI by determination of minimum bactericidal concentration.…”

Comparison of hydroalcoholic rubbing and conventional chlorhexidine scrubbing for aseptic skin preparation in dogs

“…8,32,33 Studies comparing different presurgical skin asepsis protocols in veterinary medicine are scarce. 9,10,12,29,30,[34][35][36][37][38] Commonly used methods involve a 5-minute preparation with mechanical scrubbing of the skin, during which the scrubbing procedure is repeated at least 3 times. [9][10][11][12] For our study design, CHX scrubbing was repeated 5 times because, in our hospital, we scrub twice in the preparation room and then 3 times in the operating room.…”

Virucidal Efficacy of Olanexidine Gluconate as a Hand Antiseptic Against Human Norovirus

“…Olanexidine gluconate is a novel antimicrobial compound developed by Otsuka Pharmaceutical Factory, Inc. and has been used in Japan clinically for preoperative skin preparation [Product name: Olanedine Antiseptic Solution 1.5% (OLG)] (Hagi et al 2015;Inoue et al 2015;Nakata et al 2017). The virucidal efficacy of OLG against feline calicivirus at 60-s and 10 min were 0.60, and 2.85, respectively (unpublished data).…”