Effects of off-pump versus on-pump coronary artery bypass grafting on function and viability of circulating endothelial progenitor cells

Abstract

Both on-pump and off-pump coronary artery bypass grafting elicit mobilization of endothelial progenitor cells into the peripheral blood. On-pump coronary artery bypass grafting, however, impairs the migratory function and viability of these vascular repair cells, which are conversely preserved after off-pump surgery. Further work is necessary to determine whether the function and viability of endothelial progenitor cells correlate with vascular outcomes and whether their therapeutic modulation may one day benefit coronary artery bypass grafting patients.

The effect of encapsulation of cardiac stem cells within matrix-enriched hydrogel capsules on cell survival, post-ischemic cell retention and cardiac function

“…After seven days in culture, the heterogeneous population of cells that spontaneously emigrated from the plated tissue was harvested using mild trypsinization (0.05% trypsin; Invitrogen). Circulating angiogenic cells (CACs) were isolated from peripheral blood samples donated by patients undergoing clinically indicated coronary angiography as previously described [16]. Using densitygradient centrifugation (Histopaque 1077; Sigma-Aldrich), mononuclear cells were isolated and cultured in endothelial media (EBM-2, 2% FBS, 50 ng/ml human vascular endothelial growth factor (VEGF), 50 ng/ml human insulin-like growth factor-1 and 50 ng/mL human epidermal growth factor; Clonetics).…”

Human Blood and Cardiac Stem Cells Synergize to Enhance Cardiac Repair When Cotransplanted Into Ischemic Myocardium

“…10 CACs were isolated from peripheral blood samples using standard techniques. 2,11 Normal human dermal fibroblasts (NHDFs) and human umbilical vein endothelial cells were cultured according to the manufacturer's directions.…”

578 Human Blood and Cardiac Stem Cells Synergize to Enhance Cardiac Repair When Co-Transplanted Into Ischemic Myocardium

“…This strategy reflects the logistical realities inherent in cell culture and the administration of an autologous cell product soon after myocardial infarction. Although this approach may have favored CAC function and viability, 11,13 this bias is tempered by the observation that (1) the effects of vascular damage on CAC function are very transient, (2) the results observed in both the in vivo and in vitro experiments are consistent, and (3) the phenotypic profile of both injected cells sources is consistent with those used in clinical trials ( Figure I in the online-only Data Supplement). The CSC product used in this study represents the ultimate simplification of CSC culture before antigenic selection 4 or subculture.…”

“…10 CACs were isolated from peripheral blood samples using standard techniques. 2,11 Normal human dermal fibroblasts (NHDFs) and human umbilical vein endothelial cells were cultured according to the manufacturer's directions.…”