Effects of oestrous cycle and exogenous ovarian steroids on 5‐hydroxytryptamine metabolism in rat lung.

Bakhle, Y.S.; Ben-Harari, Ruben R.

doi:10.1113/jphysiol.1979.sp012795

Cited by 18 publications

(4 citation statements)

References 12 publications

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“…From the time course of the radioactive effilux we can agree with other authors (Ryan & Smith, 1971;Crutchley et al 1978) that the nucleotides appear to remain in the vascular space and thus are most likely to be metabolized by ecto-enzymes on endothelial cells. Other membrane-associated metabolic properties of pulmonary endothelial cells like the uptake of 5-HT and the metabolism of angiotensin I and bradykinin are altered over the course of the oestrous cycle (Bakhle & Ben-Harari, 1979 a, b) and we had hoped that the membrane-associated nucleotidase activities would also show such a variation. In the event, the variation observed was small and only between two stages of the cycle.…”

Section: Discussionmentioning

confidence: 99%

“…Other membrane-associated metabolic properties of pulmonary endothelial cells like the uptake of 5-HT and the metabolism of angiotensin I and bradykinin are altered over the course of the oestrous cycle (Bakhle & Ben-Harari, 1979 a, b) and we had hoped that the membrane-associated nucleotidase activities would also show such a variation. In the event, the variation observed was small and only between two stages of the cycle.…”

Section: Discussionmentioning

confidence: 99%

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The Fate of Adenine Nucleotides in the Pulmonary Circulation of Isolated Lung

Chelliah

Bakhle

1983

Exp Physiol

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SUMMARYThe hydrolysis of ATP, ADP and ANMP was studied in perfused isolated lungs from rats, hamnsters, guinea-pigs and humaps. In rats, ATP and ADP at concentrations up to 1 mm were extensively brQken down to AMP and AMP itself wgs more resistant to further hydrqlysis. This pattern of metabolites was maintained in rat lung perfused with Krebs solution containing albumin. The effects of endogenous sex hormones on ADP metbolism were studied in lungs from female rats at different stages of the oestrous cycle. There were no cycle-related changes observed and in a comparison between sexes, only a small difference between males and females was apparent. Lung effluent from rat lunigs exhibited a low level of ADPase activity. Although hamster lungs metakolized ADP to AMP as did rat lungs, lungs from guinea-pigs and humans metabolized ADP and AMP extensively to adenosine. This difference means that although all species exhibit the ability to inactivate pro-aggregatory ADP, the ability to form the anti.aggregatory adenosine from ADP in the pulmonary vasculature varies markedly between species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Fate of Adenine Nucleotides in the Pulmonary Circulation of Isolated Lung

Chelliah

Bakhle

1983

Exp Physiol

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“…The selective pulmonary uptake of norepinephrine and serotonin, as opposed to epinephrine, is sensitive to a number of factors. Serotonin uptake is blocked by monamine oxidase inhibitors [12], while both norepinephrine and serotonin uptake are inhibited by certain steroids, halothane, nitrous oxide, anoxia, hyperoxia, and hypothermia [13][14][15]. Laboratory models of respiratory failure [16] demonstrate marked rises in circulating catacholamines in response to hypoxia and acidosis with increased pulmonary vascular resistance and systemic vascular resistance.…”

Section: Vasoactive Aminesmentioning

confidence: 99%

Research in pediatric surgery

Stolar

Altman

1985

World j. surg.

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Pediatric surgical research continues to expand along technical avenues while addressing issues of basic science. Splenic salvage is an established goal which can ordinarily be achieved without surgical intervention. Operative approaches, when indicated, can be done with minimal moribidity. Although the spleen acts as a bacterial monitor under normal circumstances by direct phagocytosis, opsonization, and modulation of macrophage function, our understanding of how the injured spleen resumes these activities remains fragmentary. Extracorporeal membrane oxygenation shows increasing promise for newborns with refractory respiratory failure. New applications of pulmonary vasoactive meidators will yield a better understanding of how this radical therapy affects cardiopulmonary homeostasis. The lungs either synthesize, modify, or pass on a number of substances including eicosanoids, vasoactive amines, and carboxypeptidases which form a resonating feedback network that controls pulmonary vascular tone. Disturbed homeostasis results in the various disease states that have pulmonary hypertension and lung injury as common features. Developments in fetal surgery have permitted creation of improved models of in utero conditions. These models variably mimic the human condition, particularly obstructive uropathy, congenital diaphragmatic hernia, and neural tube defects. Complex problems remain for patient/fetus selection and timing of surgical intervention.

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“…The preparation of lungs has been described earlier (Bakhle & Ben-Harari, 1979a). Briefly, rats were anaesthetized with pentobarbitone, and the lungs were removed and perfused via the pulmonary artery with Krebs solution, warmed to 37 0C and gassed with 95 % 02-5 % C02, at a constant flow rate of 8 ml/min.…”

Section: Introductionmentioning

confidence: 99%

Effects of the oestrous cycle on the metabolism of arachidonic acid in rat isolated lung.

Bakhle¹,

Zakrzewski²

1982

The Journal of Physiology

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SUMMARY1. The metabolism of exogenous arachidonic acid perfused through the pulmonary circulation was investigated in lungs taken from rats at different stages ofthe oestrous cycle.2. Following perfusion with [14C]arachidonic acid there was more radioactivity associated with cyclo-oxygenase products in general at pro-oestrus than at any other stage of the cycle.3. Production of6-oxo-prostaglandin Fa, and hence ofprostacyclin (PGI2) was also highest at pro-oestrus.4. Production of thromboxane B2 was highest at pro-oestrus although it was never greater than PGI2 production at any stage.5. Radioactivity retained in lung tissue was mostly present in phospholipid and free fatty acid fractions with the distribution at pro-oestrus being different from the other stages.6. Following perfusion with [14C]oleic acid (which is not a substrate for cyclooxygenase), variations in the distribution of label in radioactivity in lung were also observed. However, these were not related to the stages of the oestrous cycle in the same way as those associated with arachidonic acid.7. We conclude that both pathways of arachidonic acid metabolism in lungoxidation via cyclo-oxygenase and incorporation into phospholipid -are affected by the progress of the oestrous cycle.8. Altered arachidonate metabolism appeared to be associated chiefly with prooestrus and may be linked to those hormones involved in this stage of the oestrous cycle.

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Effects of oestrous cycle and exogenous ovarian steroids on 5‐hydroxytryptamine metabolism in rat lung.

Cited by 18 publications

References 12 publications

The Fate of Adenine Nucleotides in the Pulmonary Circulation of Isolated Lung

The Fate of Adenine Nucleotides in the Pulmonary Circulation of Isolated Lung

Research in pediatric surgery

Effects of the oestrous cycle on the metabolism of arachidonic acid in rat isolated lung.

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