Effects of oestradiol and progesterone on the metabolism of [U-14C]glucose by mouse morulae and early blastocysts in vitro

Khurana, N.K.; Wales, R. G.

doi:10.1530/jrf.0.0790267

Cited by 24 publications

(24 citation statements)

References 30 publications

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“…The lack of a progesterone effect on the uptake of 3-OMG correlates well with the finding that this hormone has no effect on energy metabolism in the mouse embryo (Khurana & Wales, 1987a). The effect of progesterone found by Perinchief (1980) is most probably a result of the supramaximal concentration of progesterone used.…”

Section: Discussionsupporting

confidence: 70%

Hexose transport in preimplantation rabbit blastocysts

Robinson¹,

Smith²,

Benos³

1990

Reproduction

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Summary. Transtrophectodermal 3-0-methyl glucose (3-0MG) transport in the rabbit blastocyst at Days 6 and 7 post coitum was investigated to understand better how the trophectoderm can regulate inner cell mass growth by controlling substrate availability. 3-0MG rapidly traversed the trophectoderm and displayed saturation kinetics (Km = 4\m=.\3 \ m=+-\ 0\m=.\5 mM, Vmax =79 \m=+-\ 3\m=.\8 nmol.cm\p=n-\2). The flux of 3-0MG was inhibited nearly 95% by 10\p=n-\4M-phloretin, and only 15% by 10\p=n-\4 M-phlorizin. Furthermore, 3\ x =r eq-\ OMG influx was inhibited by cytochalasin B (5 \g=m\M) and was unaffected by removal of sodium. The transport system had a high specificity for 2-deoxy-D-glucose and glucose, and a very low specificity for fructose and 4-\g=a\-methylglucoside. Western blots probed with a polyclonal antibody to the human erythrocyte glucose transport protein and also with a polyclonal antibody to the C-terminus of the glucose transport protein of the rat brain revealed a broad band with a molecular weight of 55 000. Using immunogold labelling techniques, Na +-independent glucose transporters were localized to both the apical and basolateral borders of the trophectodermal cell. These results suggest that the mechanism in the trophectoderm responsible for transport of glucose is similar to other sodium-independent glucose transport systems. In addition, 3-0MG influx was unaffected by short-term incubation with progesterone, the progesterone antagonist mifepristone (RU-486), PGF-2\g=a\,PGE-2, insulin, or cAMP. Day-7 p.c. embryos also transported hexoses by a similar system because the influx rate and the phlorizin/ phloretin sensitivity were the same as in the Day-6 p.c. embryo.

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Section: Discussionsupporting

confidence: 70%

Hexose transport in preimplantation rabbit blastocysts

Robinson¹,

Smith²,

Benos³

1990

Reproduction

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“…Possible endogenous substrates include lipid, glycogen and protein. Khurana & Wales (1989) have studied the breakdown of glycogen in mouse preimplantation embryos and, while there is insufficient glycogen to account for more than a few hours of oxygen uptake, this may be the source of the excess lactate produced by morula-stage embryos over and above their glucose uptake (Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

Energy metabolism in late preimplantation rat embryos

Brison¹,

Leese²

1991

Reproduction

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The consumption of pyruvate and glucose, and the production of lactate, by single preimplantation embryos, was measured using a noninvasive technique. Embryos were cultured in 300-500-nl microdrops, for 8-12 h at a time, from Day 4 to Day 6 after mating, when they developed from the 8-cell stage to expanded blastocyst. Pyruvate was the predominant substrate at the 8-cell/morula stage; glucose uptake exceeded that of pyruvate after the onset of blastocoel formation. Lactate production increased in parallel with glucose consumption. For most stages, approximately 100% of the glucose uptake was accountable for by lactate production and in some cases an additional source of lactate must be postulated. Culture in vitro had little effect on lactate production, although a lower level of metabolism was observed compared with fresh blastocysts. Rat embryos were capable of developing to blastocysts in the absence of glucose, when lactate production was greatly reduced.

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“…Glutamine and foetal calf serum (10% v/v) were added fresh on the day of use. Where progesterone was included in media, it was added as described previously (Khurana and Wales 1987a).…”

Section: Generalmentioning

confidence: 99%

“…This difference is due to the increased turnover of the polymer in utero (Edirisinghe et af. 1984) and is controlled by maternal progesterone but does not appear to be due to a direct action of steroids on embryos (Khurana and Wales 1987a).…”

Section: Introductionmentioning

confidence: 99%

Effects of Coculture with Uterine Epithelial Cells on the Metabolism of Glucose by Mouse Morulae and Early Blastocysts

Khurana¹,

Wales²

1987

Aust. Jnl. Of Bio. Sci.

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Coculture of mouse morulae/early blastocysts with isolated endometrial epithelial cells reduced incorporation of glucose carbon into embryonic glycogen but had no significant effect on incorporation into other internal carbon pools during a 5-h culture in serum-supplemented Dulbecco's modification of Eagle's minimum essential medium. Turnover of glycogen pools during 24-h chase culture of pulse-labelled embryos was unaffected by the presence of uterine epithelial cells recovered from day-4 pregnant or non-pregnant mice. However, significantly more label was retained in non-glycogen macromolecules during chase in the presence of endometrium recovered from non-pregnant than from pregnant uteri.

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Effects of oestradiol and progesterone on the metabolism of [U-14C]glucose by mouse morulae and early blastocysts in vitro

Cited by 24 publications

References 30 publications

Hexose transport in preimplantation rabbit blastocysts

Hexose transport in preimplantation rabbit blastocysts

Energy metabolism in late preimplantation rat embryos

Effects of Coculture with Uterine Epithelial Cells on the Metabolism of Glucose by Mouse Morulae and Early Blastocysts

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