Effects of obesity/fatty acids on the expression of GPR120

Abstract

Morbidly obese subjects had lower GPR120 mRNA and protein levels in VAT and a lower mRNA expression after a high-fat meal in peripheral blood mononuclear cells. The FAs effect on GPR120 mRNA and protein levels in visceral adipocytes was lower in morbidly obese subjects.

The pro-/anti-inflammatory effects of different fatty acids on visceral adipocytes are partially mediated by GPR120

“…The study included five morbidly obese (MO) and five healthy, non-obese subjects [4]. All the MO subjects underwent laparoscopic Roux-en-Y gastric bypass.…”

“…Omental VAT samples were obtained during bariatric surgery in the MO subjects and during laparoscopic surgery for hiatus hernia in the non-obese subjects [4,26,27]. VAT samples were immediately placed in phosphate buffered saline supplemented with 5 % bovine serum albumin (BSA) to perform the adipocyte isolation and in vitro incubations.…”

“…Moreover, a GPR-120-selective agonist improved chronic inflammation in obese mice [14]. Also, GPR120 levels in adipocytes can be differently modulated by FAs [4].…”

“…The influence of diet and its fatty acids (FAs) composition on adipose tissue development and distribution is well known [3]. However, specific classes of FAs may have differential effects [4][5][6]. While saturated FAs are pro-inflammatory [7], n-3 FAs (docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)), the major ingredients in fish oil exert a potent anti-inflammatory effect [8].…”

The expression of genes involved in jejunal lipogenesis and lipoprotein synthesis is altered in morbidly obese subjects with insulin resistance

“…17 Also, a cell viability assay was performed in triplicate using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Southampton, UK) according to the manufacturer's instructions.…”