Effects of nutritional deprivation on juvenile green sturgeon growth and thermal tolerance

Verhille, Christine E.; Lee, Seunghyung; Todgham, Anne E.; Cocherell, Dennis E.; Hung, Silas S.O.; Fangue, Nann A.

doi:10.1007/s10641-015-0463-8

Cited by 13 publications

(13 citation statements)

References 70 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fish were allowed a 1-hr adjustment period before water temperature was increased at a rate of 0.3°C/min (Fangue, Hofmeister, & Schulte, 2006;Sardella, Sanmarti, & Kültz, 2008;Verhille, Todgham, Hung, & Fangue, 2015). The CTMax test chamber was an insulated, 11.0 cm), filled with 100 ml of water matching thermal acclimation temperature.…”

Section: Critical Thermal Maximamentioning

confidence: 99%

“…Food deprivation typically results in low long-term and shortterm energy substrates, and less energy devoted towards somatic growth (Verhille et al, 2015). In isolation, food restriction resulted in a 30.6% reduction in SGR at 18°C.…”

Section: Isolated and Combined Effects Of Stressors On Sgrmentioning

confidence: 99%

“…Water within chambers was constantly aerated by running airlines attached to pipette tips (10 μl, Thermo Fisher Scientific, Waltham, U.S.A.) into each chamber. Fish were allowed a 1-hr adjustment period before water temperature was increased at a rate of 0.3°C/min (Fangue, Hofmeister, & Schulte, 2006;Sardella, Sanmarti, & Kültz, 2008;Verhille, Todgham, Hung, & Fangue, 2015). Chamber water temperatures were monitored and recorded using an alcohol thermometer calibrated against a certified standard mercury thermometer (Thermo Fisher Scientific), and a calibrated optic shuttle temperature logger (Onset Computer Corporation, Bourne, MA, U.S.A.) also recorded temperature every 15 s. Fish were observed throughout the trials and loss of equilibrium (LOE), defined as an inability to maintain an upright position in the water column for 10 s, was used as an endpoint (Lee et al, 2016).…”

Section: Critical Thermal Maximamentioning

confidence: 99%

See 2 more Smart Citations

Stressor interactions in freshwater habitats: Effects of cold water exposure and food limitation on early‐life growth and upper thermal tolerance in white sturgeon, Acipenser transmontanus

et al. 2018

Self Cite

View full text Add to dashboard Cite

Limited food availability and altered thermal regimes (e.g. cold water releases from dams) are two common stressors threatening the persistence of fishes inhabiting anthropogenically disturbed freshwater systems. Yet, the combined effects of these stressors remain poorly characterised. To remedy this, we examined the isolated and combined effects of low temperature exposure and food restriction on specific growth rate (SGR, % body mass/day) and upper thermal tolerance (critical thermal maxima, CTMax) in larval white sturgeon (Acipenser transmontanus [Acipenseridae], 32 days post‐hatch, body mass: 0.25 ± 0.03 g, mean ± standard deviation). A 2 × 2 factorial design was implemented with fish exposed to one of two ecologically‐relevant acclimation temperatures (cold exposure: 11°C or a control temperature: 18°C) and one of two food restriction treatments designed to emulate observed declines in food availability (100% or 40% optimal feed rate) for 6 weeks (N: 3 replicate tanks/treatment, 50 fish/tank). Specific growth rate was affected by both low temperature exposure and food restriction in isolation; low temperature exposure reduced SGR by 56.5% and food restriction reduced SGR by 30.6%. Simultaneous exposure to low temperature and food restriction resulted in a greater but less than additive reduction in SGR (80.6%), indicating that the stressors interacted antagonistically. Critical thermal maxima were c. 2°C higher in 18°C‐acclimated fish (CTMax = 30.7 ± 0.4°C, mean ± standard error) compared to 11°C‐acclimated fish (CTMax = 28.6 ± 0.2°C, mean ± standard error); however, CTMax was independent of food restriction in both 11°C‐ and 18°C‐acclimated fish. These data highlight the unpredictability of stressor interactions and may guide holistic conservation strategies, which target co‐occurring stressors in freshwater habitats.

show abstract

Section: Critical Thermal Maximamentioning

confidence: 99%

Section: Isolated and Combined Effects Of Stressors On Sgrmentioning

confidence: 99%

Section: Critical Thermal Maximamentioning

confidence: 99%

See 1 more Smart Citation

Stressor interactions in freshwater habitats: Effects of cold water exposure and food limitation on early‐life growth and upper thermal tolerance in white sturgeon, Acipenser transmontanus

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…The upper temperature tolerance of feed-restricted (12.5%, 25%, 50% OFR) and non-feed-restricted (100% OFR) green and white sturgeon juveniles were determined following standard CTMax methodology for teleost fishes (0.3°C/min; e.g., Fangue and Bennett, 2003) and used previously in sturgeon research (Sardella et al, 2008;Verhille et al, 2015). Following the four-week feed restriction trial, fish from each feed restriction group (N = 12, four fish per replicate tank for a given feed restriction group) were individually placed in one of four half-full, 57 L glass aquaria for 30 min (18.6 ± 0.5°C, mean ± SEM).…”

Section: Critical Thermal Maximum (Ctmax)mentioning

confidence: 99%

“…Levels of Hsc/Hsp70 were measured using the discontinuous SDS-PAGE method of Laemmli (1970) followed by immunoblotting as detailed by Verhille et al (2015) and modified from Todgham et al (2005) and Deng et al (2009). Amount of total protein used for SDS-PAGE varied depending on tissue type and sturgeon species: 7.5 and 13 μg (gill), 7.5 and 5 μg (brain), and 10 and 5 μg (mucus) for green and white sturgeon, respectively.…”

Section: -Kda Heat Shock Protein (Hsc/hsp70) Response To Elevated Tmentioning

confidence: 99%

Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeon

Lee

Hung

Fangue

et al. 2016

Comparative Biochemistry and Physiology Part A: Molecular &

Self Cite

View full text Add to dashboard Cite

The objective of the current study was to investigate the effects of feed restriction on whole-organism upper thermal tolerance and the heat shock response of green and white sturgeon to determine how changes in food amount might influence physiological performance of each species when faced with temperature stress. Two parallel feed restriction trials were carried out for juvenile green (202 g; 222-day post hatch: dph) and white sturgeon (205 g; 197-dph) to manipulate nutritional status at 12.5%, 25%, 50%, or 100% of optimum feeding rate (100% OFR were 1.6% and 1.8% body weight/day, respectively) for four weeks. Following the trials, the critical thermal maximum (CTMax, 0.3°C/min) of sturgeon (N = 12/treatment/species) was assessed as an indicator of whole-organism upper thermal tolerance. To assess temperature sensitivity, sturgeon (N = 9/treatment/species) were acutely transferred to two temperature treatments (28°C and 18°C as a handling control) for 2 h followed by 2 h of recovery at 18°C before being sacrificed, and gill, brain, and mucus sampled for measurements of 70-kDa heat shock protein levels (Hsc/Hsp70). Feeding rate had species-specific effects on CTMax in green and white sturgeon such that CTMax of green sturgeon decreased as the magnitude of feed restriction increased; whereas, CTMax of white sturgeon did not change with feed restriction. Elevated temperature (28°C) and feed restriction increased Hsc/Hsp70 levels in the gill tissue of green sturgeon, while heat shock increased Hsc/ Hsp70 levels in the mucus of white sturgeon. Our results suggest that green sturgeon may be more susceptible to temperature stress under food-limited conditions.

show abstract

Dietary Restriction, Starvation, Compensatory Growth – ‘Short-Term Fasting Does Not Kill You: It Can Make You Stronger’

Steinberg

2018

Aquatic Animal Nutrition

View full text Add to dashboard Cite

Effects of nutritional deprivation on juvenile green sturgeon growth and thermal tolerance

Cited by 13 publications

References 70 publications

Stressor interactions in freshwater habitats: Effects of cold water exposure and food limitation on early‐life growth and upper thermal tolerance in white sturgeon, Acipenser transmontanus

Stressor interactions in freshwater habitats: Effects of cold water exposure and food limitation on early‐life growth and upper thermal tolerance in white sturgeon, Acipenser transmontanus

Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeon

Dietary Restriction, Starvation, Compensatory Growth – ‘Short-Term Fasting Does Not Kill You: It Can Make You Stronger’

Contact Info

Product

Resources

About