Effects of nutrients on anthocyanin production in cultured cells of Aralia cordata

Sakamoto, Kazuo; Iida, Kumiko; Sawamura, Kimiko; Hajiro, Kyoko; Asada, Yujiro; Yoshikawa, Takafumi; Furuya, Tsutomu

doi:10.1016/0031-9422(93)85517-u

Cited by 70 publications

(30 citation statements)

References 8 publications

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“…Anthocyanin production was considerably greater with EM medium than with MS medium at all sugar concentrations. We hypothesize that the ratio of NH 4 ϩ and NO 3 Ϫ may be one of the important factors in culture media because it has been reported that in several plant species a nitrogen source improves anthocyanin production by changing primary metabolism of the cells (Sakamoto et al 1993, Sato et al 1996. When comparing 3% to 6% sugar in EM medium, 6% sugar resulted in the highest anthocyanin production (Figure 3).…”

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confidence: 97%

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Hennayake

Takagi

Nishimura

et al. 2006

Plant Biotechnology

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A suspension cultured cell line was established from the cultivar of Rosa hybrida 'Charleston' as a study model to understand the response of the anthocyanin biosynthesis pathway to environmental cues. The major identified anthocyanin in cell cultures was cyanidin 3-glucoside (chrysanthemin). The anthocyanin yield was enhanced by culturing cells in the EM medium with added sucrose at high concentration under additional UV-B radiation to white light. Three cDNA fragments were cloned with degenerate primers by RT-PCR and the obtained sequences shared high homology with putative key enzymes (DFR, ANS, and UF3GT) of other species. The expression levels of these three genes were promoted under optimum conditions for anthocyanin accumulation. These results suggest that expression levels of these genes were closely correlated with a temporal buildup of anthocyanins in response to environmental factors.Key words: Anthocyanin, chrysanthemin, cyanin, Rosa hybrida, suspension cell culture.Plant Biotechnology 23, 379-385 (2006) Original PaperThis article can be found at http://www.jspcmb.jp/ pelargonidin, cyanidin and peonidin type anthocyanins (Biolley et al. 1994). Recently, tissue culturing has become an important biotechnological technique to produce various secondary metabolites including anthocyanins on a mass scale for industrial and academic interest (Hirner et al. 2001;Gantet and Memelink 2002). Despite the fact that some plant species have been shown to produce anthocyanins in cell cultures, little information of the cell culture techniques that are capable of producing in vitro anthocyanins has been reported in rose.The biosynthesis of anthocyanins in plant cell culture is stimulated by not only genetic factors but also environmental factors such as light, temperature, growth regulators and nutrition (Sato et al. 1996;Zhang et al. 1997;Zhang et al. 2002). The identity of the environmental stimuli that lead to the accumulation of anthocyanins in rose is of considerable interest. To date, Sato et al. (1996) has examined the intensity of white light that induces anthocyanin production in strawberry cell culture lines; however, no information was obtained about the effect of light quality on the production of anthocyanins in cultural cells. Therefore, the originality of this research is to investigate anthocyanin biosynthesis in cultural cells exposed to UV-B (290-320 nm) radiation.In this study, we used Rosa hybrida cultivar 'Charleston', a climbing type, originated in Australia. The open flowers of 'Charleston' undergo a striking color change from yellow to red over 10-12 days under natural daylight. We established a suspension cultured cell line from this cultivar of rose. Anthocyanin content was quantified by HPLC to determine the most efficient conditions, such as light including UV-B radiation and media, for continuous production of the pigments in the rose cell suspension. The expression levels of three genes encoding putative key enzymes (DFR, ANS, and UF3GT) for anthocyanin biosynthesis were ana...

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confidence: 97%

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Hennayake

Takagi

Nishimura

et al. 2006

Plant Biotechnology

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“…These include callus cultures of Ajuga reptans flowers (Callebaut et al, 1997), callus cultures of Aralia cordata Thunb. (Sakamoto et al, 1993), Vitis sp. cells in suspension culture (Yamakawa et al, 1983).…”

Section: Discussionmentioning

confidence: 99%

“…cells in suspension culture (Yamakawa et al, 1983). In Aralia cordata the highest anthocyanin yield was obtained when medium containied 1.0 mg·l -1 2,4-D and 0.1 mg·l -1 kinetin (Sakamoto et al, 1993). Some Solanum tuberosum L. cultivars can also produce anthocyanin in darkness but light enhanced the level of these pigments (Lewis et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Elicitation of Anthocyanin Production in Roots of Kalanchoe blossfeldiana by Methyl Jasmonate

Góraj-Koniarska

Stochmal

Oleszek

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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The influence of methyl jasmonate on anthocyanin accumulation in roots of Kalanchoe blossfediana plants was studied. Methyl jasmonate (JA-Me), at a concentration of 5.0 to 40.0 mg.l -1 , substantially increased anthocyanin accumulation in roots of intact plants, when it was applied as a solution under natural light conditions. The production of anthocyanin depended on the concentration of methyl jasmonate and the age of the plant. The stimulatory effect was higher in older plants of K. blossfeldiana than in younger ones. When leaves were removed methyl jasmonate slightly stimulated anthocyanin accumulation compared with intact plants. The obtained results indicate that leaves are necessary for the anthocyanin accumulation in the roots. In isolated roots methyl jasmonate did not affect the accumulation of anthocyanins in light conditions. Seven anthocyanins were documented in the roots of control plants and 8 anthocyanins in the roots of JA-Me treated ones. JA-Me increased the level of anthocyanins in roots of old K. blossfeldiana plants 6.8, 6.0 and 3.6-folds, after 4, 8 and 14-days of treatment, respectively.

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“…Although the effects of light have been shown to induce anthocyanin biosynthesis in many plant species, anthocyanin biosynthesis in some species, such as Aralia cordata (Sakamoto et al 1993), Fragaria ananassa (Nakamura et al 1999), and sweet potato (Konczak-Islam et al 2000), was achieved in the dark. Chan et al (2010) claimed that dark/light cycling had a prominent effect on anthocyanin production, in which cells cultured under continuous lighting/darkness did not strongly induce anthocyanin; however, cultures that were exposed to darkness, followed by exposure to light produced high anthocyanin content.…”

Section: Introductionmentioning

confidence: 99%

Influences of different light sources and light/dark cycles on anthocyanin accumulation and plant growth in Petunia

Naing

Kim

2016

J Plant Biotechnol

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Anthocyanin accumulation and plant growth were examined in petunia (NT and T 2 transgenic plants) by determining the effects of different sources of light and varying light/dark cycles. Red light significantly enhanced anthocyanin content of B-peru+mPAP1; however, it had a negative effect on anthocyanin production in RsMYB1 plants. In general, white light was found to be reasonable for anthocyanin accumulation in all plants. In case of light/dark cycles, application of seven days of light:14 days of dark significantly enhanced anthocyanin content. We found that anthocyanin content detected in transgenic plants expressing anthocyanin regulatory transcription factor genes (B-peru+mPAP1 or RsMYB1) was higher than that in NT plants in all treatments. Plant growth was also influenced by the different light sources and dark/light cycles. Taken together, our results suggest that light source and light/dark cycle play an important role in anthocyanin production and plant growth. The choice of the optimal conditions is also important for anthocyanin production and plant growth depending on NT or transgenic plants carrying anthocyanin regulatory transcription factors.

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Effects of nutrients on anthocyanin production in cultured cells of Aralia cordata

Cited by 70 publications

References 8 publications

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Elicitation of Anthocyanin Production in Roots of Kalanchoe blossfeldiana by Methyl Jasmonate

Influences of different light sources and light/dark cycles on anthocyanin accumulation and plant growth in Petunia

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