Effects of nutrients, cell density and culture techniques on protoplast regeneration and early protonema development in a moss,Physcomitrella patens

Schween, Gabriele; Hohe, Annette; Kopřivová, Anna; Reski, Ralf

doi:10.1078/0176-1617-00855

Cited by 62 publications

(45 citation statements)

References 16 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These have been discussed in several reviews on this topic (Bopp and Bhatla 1990;Cove and Knight 1993;Cove et al 1997;Reski 1998b). More recent publications in this field comprise polarity induction (Bhatla et al 2002), protoplast regeneration (Schween et al 2003a) and photoperiodism .…”

Section: Physcomitrella As a Model Systemmentioning

confidence: 99%

A tool for understanding homologous recombination in plants

Hohe

Reski

2003

Plant Cell Reports

View full text Add to dashboard Cite

Attempts for establishing an efficient gene targeting (GT) system in seed plants have hitherto not been successful. In contrast, GT based on homologous recombination is highly efficient in Physcomitrella, making this moss a novel tool in reverse genetics. However, why homologous and illegitimate recombination are differently regulated between Physcomitrella and seed plants is still enigmatic. Here we update the state of the art of GT in Physcomitrella and discuss approaches to unravel this enigma. Identification of molecular factors significantly enhancing GT and their subsequent transfer to crop plants will have a great impact on plant biotechnology by enabling precise genetic engineering. Physcomitrella appears to be the most useful model system in this context.

show abstract

Section: Physcomitrella As a Model Systemmentioning

confidence: 99%

A tool for understanding homologous recombination in plants

Hohe

Reski

2003

Plant Cell Reports

View full text Add to dashboard Cite

show abstract

“…After 6 to 7 d of growth, chloronemal apical cells begin to differentiate into caulonemal cells. Caulonemal cells are defined as being relatively chloroplast poor and having oblique cell plates (Reski, 1998;Schween et al, 2003;Menand et al, 2007;Pressel et al, 2008). Observing protonemata growing within microfluidic chambers affords the opportunity to watch this differentiation occur.…”

Section: Microfluidic Chambers Allow For Observation Of Developmentalmentioning

confidence: 99%

Long-Term Growth of Moss in Microfluidic Devices Enables Subcellular Studies in Development

Bascom

Nelson

et al. 2016

Plant Physiol.

View full text Add to dashboard Cite

Key developmental processes that occur on the subcellular and cellular level or occur in occluded tissues are difficult to access, let alone image and analyze. Recently, culturing living samples within polydimethylsiloxane (PDMS) microfluidic devices has facilitated the study of hard-to-reach developmental events. Here, we show that an early diverging land plant, Physcomitrella patens, can be continuously cultured within PDMS microfluidic chambers. Because the PDMS chambers are bonded to a coverslip, it is possible to image P. patens development at high resolution over long time periods. Using PDMS chambers, we report that wild-type protonemal tissue grows at the same rate as previously reported for growth on solid medium. Using longterm imaging, we highlight key developmental events, demonstrate compatibility with high-resolution confocal microscopy, and obtain growth rates for a slow-growing mutant. By coupling the powerful genetic tools available to P. patens with long-term growth and imaging provided by PDMS microfluidic chambers, we demonstrate the capability to study cellular and subcellular developmental events in plants directly and in real time.

show abstract

“…As a complex solid growth medium, sterilised peat soil is used and, commonly, either liquid or solidified simple mineral media basically containing KH 2 PO 4 , MgSO 4 , Ca(NO 3 ) 2 , or KNO 3 and FeSO 4 with a pH ranging from 4.5 to 6.5 as defined growth media (Reski et al, 1994). These media are often supplemented with defined microelements (Ashton et al, 1979;Nishiyama et al, 2000) and, depending on the need of the cultivated line, with vitamins or a carbon source (Schween et al, 2003). Light and temperature are controlled to maintain standardised culture conditions.…”

Section: Cultivation and Growthmentioning

confidence: 99%

The moss Physcomitrella patens: methods and tools from cultivation to targeted analysis of gene function

Strotbek¹,

Krinninger²,

Frank³

2013

Int. J. Dev. Biol.

View full text Add to dashboard Cite

To comprehensively understand the major processes in plant biology, it is necessary to study a diverse set of species that represent the complexity of plants. This research will help to comprehend common conserved mechanisms and principles, as well as to elucidate those mechanisms that are specific to a particular plant clade. Thereby, we will gain knowledge about the invention and loss of mechanisms and their biological impact causing the distinct specifications throughout the plant kingdom. Since the establishment of transgenic plants, these studies concentrate on the elucidation of gene functions applying an increasing repertoire of molecular techniques. In the last two decades, the moss Physcomitrella patens joined the established set of plant models based on its evolutionary position bridging unicellular algae and vascular plants and a number of specific features alleviating gene function analysis. Here, we want to provide an overview of the specific features of P. patens making it an interesting model for many research fields in plant biology, to present the major achievements in P. patens genetic engineering, and to introduce common techniques to scientists who intend to use P. patens as a model in their research activities.

show abstract

Effects of nutrients, cell density and culture techniques on protoplast regeneration and early protonema development in a moss,Physcomitrella patens

Cited by 62 publications

References 16 publications

A tool for understanding homologous recombination in plants

A tool for understanding homologous recombination in plants

Long-Term Growth of Moss in Microfluidic Devices Enables Subcellular Studies in Development

The moss Physcomitrella patens: methods and tools from cultivation to targeted analysis of gene function

Contact Info

Product

Resources

About