Effects of Notch ligand Delta1 on the proliferation and differentiation of human dental pulp stem cells in vitro

He, Fei; Yang, Zheng; Tan, Yinghui; Yu, Na; Wang, Xuefei; Yao, Naihui; Zhao, Jiaju

doi:10.1016/j.archoralbio.2008.10.003

Cited by 38 publications

(38 citation statements)

References 30 publications

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“…Interestingly, a large number of regulatory genes in these differentiation interact or crosstalk via Notch, Wnt, transforming growth factor beta (TGF-beta)/bone morphogenic protein (BMP), and cadherin signaling pathways to play a crucial role in their determination (He et al, 2009;Liu et al, 2009). Moreover, it was reported that implantation of DPSCs induce endogenous axon guidance and induced neuroplasticity within a receptive host nervous system (Arthur et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Induced in vitro differentiation of neural-like cells from human exfoliated deciduous teeth-derived stem cells

Nourbakhsh¹,

Soleimani²,

Taghipour³

et al. 2011

Int. J. Dev. Biol.

104

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Section: Discussionmentioning

confidence: 99%

Induced in vitro differentiation of neural-like cells from human exfoliated deciduous teeth-derived stem cells

Nourbakhsh¹,

Soleimani²,

Taghipour³

et al. 2011

Int. J. Dev. Biol.

104

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“…These include stem cells from exfoliated deciduous teeth (SHEDs), periodontal ligament stem cells (PDLSCs), dental follicle progenitor cells (DFPCs) and stem cells from apical papilla (SCAPs). These post-natal populations have mesenchymal stem cell (MSC)-like qualities, namely the capacity for self-renewal, the potential to differentiate into multiple lineages, including osteoblasts and chondroblasts, and a potential for in vitro differentiation into cell types from various embryonic layers, including adipose, bone, endothelial and neural-like tissues (Arthur et al, 2008;Cheng et al, 2008;Cordeiro et al, 2008;Fujii et al, 2008;Gay et al, 2007;Harada et al, 1999;He et al, 2009;Honda et al, 2008;Huo et al, 2010). Many researchers have proposed that DPMSCs are promising candidates for the repair and regeneration of a variety of mesenchymal tissues, such as bone, cartilage and muscle (Dezawa et al, 2005;Noël et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Dental Pulp of the Third Molar: A New Source of Pluripotent-like Stem Cells

Atari

Gil-Recio

Fabregat

et al. 2012

Journal of Cell Science

107

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SummaryDental pulp is particularly interesting in regenerative medicine because of the accessibility and differentiation potential of the tissue. Dental pulp has an early developmental origin with multi-lineage differentiation potential as a result of its development during childhood and adolescence. However, no study has previously identified the presence of stem cell populations with embryonic-like phenotypes in human dental pulp from the third molar. In the present work, we describe a new population of dental pulp pluripotent-like stem cells (DPPSCs) that were isolated by culture in medium containing LIF, EGF and PDGF. , and they show genetic stability in vitro based on genomic analysis with a newly described CGH technique. Interestingly, DPPSCs were able to form both embryoid-body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ layers when injected in nude mice. We examined the capacity of DPPSCs to differentiate in vitro into tissues that have similar characteristics to mesoderm, endoderm and ectoderm layers in both 2D and 3D cultures. We performed a comparative RT-PCR analysis of GATA4, GATA6, MIXL1, NANOG, OCT3/4, SOX1 and SOX2 to determine the degree of similarity between DPPSCs, EBs and human induced pluripotent stem cells (hIPSCs). Our analysis revealed that DPPSCs, hIPSC and EBs have the same gene expression profile. Because DPPSCs can be derived from healthy human molars from patients of different sexes and ages, they represent an easily accessible source of stem cells, which opens a range of new possibilities for regenerative medicine.

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“…The activation of NOTCH signaling by either Jagged1 or N1ICD inhibits odontoblast differentiation of DPSCs without affecting their proliferation [97]. Therefore, NOTCH signaling pathway plays an important role in maintaining the correct balance between proliferation and differentiation of DPSCs [96].…”

Section: Notch Signaling Pathwaymentioning

confidence: 99%

“…NOTCH ligand Delta1 is known to influence the proliferation and differentiation of many types of tissue specific stem cells. NOTCH-Delta1 signaling is expressed in human DPSCs and can enhance the proliferation of DPSCs [96]. The activation of NOTCH signaling by either Jagged1 or N1ICD inhibits odontoblast differentiation of DPSCs without affecting their proliferation [97].…”

Section: Notch Signaling Pathwaymentioning

confidence: 99%