Effects of norepinephrine on hypoperfusion-reperfusion injuries in hearts isolated from normal and diabetic rats

Higuchi, Makie; Ikema, Shoko; Matsuzaki, Toshihiro; Hirayama, Kiyotake; Sakanashi, Matao

doi:10.1016/0022-2828(91)90101-q

Cited by 13 publications

(6 citation statements)

References 29 publications

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“…The depressed de veloped LVP in the pre-treated group would not be caused by the increase in lactate con tent because lactate also increased in the post-treated group in which developed LVP was the same as that in the non-treated group. These results are consistent with the results in our previous studies (9,10), in which de veloped LVP was lower along with lower myocardial stiffness and higher lactate con tent in non-diabetic hearts than in diabetic hearts. Developed LVP may be associated with not only contractile function but also the resting condition of the hearts.…”

Section: Animals and Treatmentsupporting

confidence: 93%

“…As described in our previous study (9), NE aggravated the cardiac dysfunction during hypoperfusion in hearts isolated from diabetic rats. Therefore, it is also conceivable that such a protective effect of insulin on NE injury can be attributable to the beneficial effects in the present study.…”

Section: Animals and Treatmentsupporting

confidence: 77%

“…The plasma glucose level measured by the enzymatic method (Gluneo, Shinotest Co., Ltd.) was 485±1 3 mg/dl. Then after thoracotomy, the hearts were quickly removed and perfused with the Langendorff apparatus as described in the previous report (9). The perfusate was Krebs-Henseleit solu tion (pH 7.4, 36°C) containing 120 mM NaCI, 4.8 mM KCI, 1.25 mM CaC12, 1.2 mM MgSO4, 1.2 mM KH2PO4, 11 mM glucose and 25 mM NaHCO3, equilibrated with 95% 02 5% C02…”

Section: Animals and Treatmentmentioning

confidence: 99%

“…Six hearts were hypoper fused without insulin (non-treated group, n=6). At the end of hypoperfusion, the hearts were quickly frozen in liquid nitrogen for measurement of tissue substrates as described previously (9).…”

Section: Animals and Treatmentmentioning

confidence: 99%

“…In our previous studies (9,10), the hearts isolated from diabetic rats were more vulner able to hypoperfusion and more susceptible to norepinephrine (NE) under hypoperfusion in comparison with the hearts isolated from non-diabetic rats. These dysfunctions ob served in diabetic hearts were improved to non-diabetic heart levels by daily administra tion of insulin.…”

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confidence: 98%

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Improvement of Hypoperfusion with Norepinephrine Injury by Ex Vivo Insulin in Isolated Diabetic Rat Hearts

Ikema

Higuchi

Hirayama

et al. 1990

Japanese Journal of Pharmacology

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Abstract-Effects of insulin on contractile and energy metabolic dysfunctions during hypoperfusion (2 ml/min/g heart wt., 60 min) with 10-6 M norepinephrine were studied in paced hearts isolated from streptozotocin-diabetic rats. Insulin (2 mU/ min/g heart wt.) was infused 20 min before and during hypoperfusion (pre-treated group) or 30 min after the onset of hypoperfusion (post-treated group). Hearts in the non-treated group were hypoperfused without insulin and other hearts in the control group were not hypoperfused.In the non-treated group, resting con tractile force (CF) and resting left ventricular pressure (LVP) were significantly elevated to maximum levels within 30 min after hypoperfusion and these elevations were restored in the pre-treated group but not in the post-treated group. Developed CF was depressed in the non-treated group and improved significantly in the pre treated group but not in the post-treated group. Developed LVP was depressed in the non-treated group, and depression was slightly larger in the pre-treated group. In the non-treated group, ATP and creatine phosphate contents in the left ventricle significantly decreased. Decreases in ATP and creatine phosphate contents in the inner layer were partially restored in the pre-treated group but not in the post treated group. Lactate significantly increased in the non-treated group and increased even further in the insulin treated groups.These results indicate that contractile dysfunction during hypoperfusion with norepinephrine is improved by pre-treated insulin, as is partial recovery of energy metabolism.

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Section: Animals and Treatmentsupporting

confidence: 93%

Section: Animals and Treatmentsupporting

confidence: 77%

Section: Animals and Treatmentmentioning

confidence: 99%

Section: Animals and Treatmentmentioning

confidence: 99%

mentioning

confidence: 98%

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