Effects of non-starch polysaccharides enzymes on pancreatic and small intestinal digestive enzyme activities in piglet fed diets containing high amounts of barley

Abstract

AIM:To investigate effects of non-starch polysaccharides(NSP) enzymes on pancreatic and small intestinal digestive enzyme activities in piglet fed diets containing high amounts of barley. METHODS:Sixty crossbred piglets averaging 13.5 kg were randomly assigned to two treatment groups with three replications (pens) based on sex and mass. Each group was fed on the diet based on barley with or without added NSP enzymes (0.15%) for a 40-d period. At the end of the experiment the pigs were weighed. Three piglets of each group were chosen and slaughtered. Pancreas, digesta from the distal end of the duodenum and jejunal mucosa were collected for determination. Activities of the digestive enzymes trypsin, chymotrypsin, amylase and lipase were determined in the small intestinal sections as well as in homogenates of pancreatic tissue. Maltase, sucrase, lactase and γ-glutamyl transpeptidase (γ-GT) activities were analyzed in jejunal mucosa. RESULTS:Supplementation with NSP enzymes improved growth performance of piglets. It showed that NSP enzymes had no effect on digestive enzyme activities in pancreas, but decreased the activities of proteolytic enzyme, trypsin, amylase and lipase in duodenal contents by 57.56%, 76.08%, 69.03% and 40.22%(P<0.05) compared with control, and increased γ-GT activities in jejunal mucosa by 118.75%(P<0.05). CONCLUSION:Supplementation with NSP enzymes in barley based diets could improve piglets' growth performance, decrease activities of proteolytic enzyme, trypsin, amylase and lipase in duodenal contents and increase γ-GT activities in jejunal mucosa.Li WF, Feng J, Xu ZR, Yang CM. Effects of non-starch polysaccharides enzymes on pancreatic and small intestinal digestive enzyme activities in piglet fed diets containing high amounts of barley.

Effects of supplementation of xylanase, phospholipase or combination of both to a wheat-based diet on digestive function in early-weaned piglets

“…There was no significant effect (P>0.05) of the supplementation with xylanase on the activities of brush-border enzymes (Table 2). These results agree, in part, with those by Li et al (2004) who Differences compared to the control diet within rows are marked † for tendencies (P≤0.10) 2 leucine aminopeptidase reported that the supplementation of pig diets based on barley with both xylanase and β-glucanase caused a significant increase in activity of γ-glutamyl transpeptidase in the jejunal mucosa only, whereas activities of disaccharidases, including maltase and lactase, were not significantly changed. Moreover, the authors showed that exogenous non starch polysaccharides (NSP) degrading enzymes decreased significantly activities of pancreatic enzymes, including α-amylase and lipase, in digesta obtained from the distal part of the duodenum.…”

Effect of Non-Starch Polysaccharide (NSP) of Wheat and Barley Supplemented with Exogenous Enzyme Blend on Growth Performance, Gut Microbial, Pancreatic Enzyme Activities, Expression of Glucose Transporter (SGLT1) and Mucin Producer (MUC2) Genes of Broiler Chickens

“…These results reflect the fact that water-soluble NSP of wheat and barley impede pancreatic α-amylase and lipase activities (Li et al, 2004). This mode may indicate needs for greater secretion of pancreatic enzymes (Williams, 1996;Denbow, 2000).…”

“…This mode may indicate needs for greater secretion of pancreatic enzymes (Williams, 1996;Denbow, 2000). Based on relevant research findings intestinal enzyme activity depends on the original of dietary nutrient and quantity or quality of anti-nutrients in the gut (Li et al, 2004;Mirzaie et al, 2012). Also diet issue affects the magnitude of secretion…”

“…For the pancreatic enzyme activity assay, sample sections (3 cm in length) were taken from the middle of the pancreas, rinsed with 0.01 M PBS (pH 7.2), and stored in liquid nitrogen at -80ºC until analysis. The pancreatic samples were homogenized in ice-cold 0.2 mol/L TrisHcl buffer, containing 0.05 mol/L NaCl as described by Li et al (2004). The homogenates were centrifuged at 3000 rpm for 15 min at 4ºC and the supernatants were saved for ulterior spectrophotometric measurement.…”

Effect of Italian heavy pig diets based on different barley varieties with or without non-starch polysaccharides degrading enzymes on growth performance, carcass characteristics and fresh thigh quality

“…The most of the studies focuses on the use of exogenous NSP enzymes in diets for weaned piglets, as especially piglets do not produce enzymes capable of degrading NSP (Kerr & Shurson 2013). Li et al (2004) and Fan et al (2009) reported that the addition of b-glucanase and xylanase to barley-based diets improved growth performance of piglets. However, the need for exogenous NSP enzyme supplementation of barley-based diets on weaned piglets can depend on the used barley variety (Prandini et al 2014).…”