Effects of Noise and Salicylate on Hair Cell Loss in the Chinchilla Cochlea

Spongr, Vlasta P.; Boettcher, Flint A.; Saunders, Samuel S.; Salvi, Richard

doi:10.1001/archotol.1992.01880020051015

Cited by 28 publications

(12 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, we found no significant difference in the mean ABR threshold shift between the salicylate and control groups. Previous morphological studies have also revealed that salicylate did not change the structure of the inner ear or the number or distribution of IHCs [1,13,14,44]. In the present study, the fact that salicylate did not change ABR thresholds indicates that salicylate did not cause any hearing impairment, which should be avoided in the study of tinnitus.…”

Section: Discussionsupporting

confidence: 60%

“…Boettcher and Salvi reported that treatment with 300-450 mg/kg salicylate did not cause hair cell loss in chinchillas [13]. The same results were reported in other research, demonstrating that salicylate does not alter the number or distribution of hair cells [13,14]. Other authors reported that salicylate might inhibit the electromotility of outer hair cells, resulting in hearing loss and tinnitus [15,16].…”

Section: Introductionmentioning

confidence: 52%

“…However, morphological studies found no obvious anatomical changes in the structure of inner ear, including the hair cells and vascular and supporting tissues in the cochlea [1]. Boettcher and Salvi reported that treatment with 300-450 mg/kg salicylate did not cause hair cell loss in chinchillas [13]. The same results were reported in other research, demonstrating that salicylate does not alter the number or distribution of hair cells [13,14].…”

Section: Introductionmentioning

confidence: 62%

See 2 more Smart Citations

Changes in the Numbers of Ribbon Synapses and Expression of RIBEYE in Salicylate-Induced Tinnitus

Zhang

Xue

Liu

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: This study was performed to explore the mechanism underlying tinnitus by investigating the changes in the synaptic ribbons and RIBEYE expression in cochlear inner hair cells in salicylate-induced tinnitus. Methods: C57BL/6J mice were injected with salicylate (350 mg/kg) for 10 days and grouped. Behavioral procedures were performed to assess whether the animals experienced tinnitus. The specific presynaptic RIBEYE protein and non-specific postsynaptic glutamate receptor 2&3 protein in basilar membrane samples were examined by immunofluorescent labeling. RT-PCR and Western blot assays were used to examine RIBEYE expression. Serial sections were used to build three-dimensional models using 3ds MAX software to evaluate the changes in the synaptic ribbons. Results: The administration of salicylate increased false positives in the behavioral procedure from 3 d to 10 d. The membrane profiles of inner hair cells in all mice were intact. The number of synaptic ribbons in the salicylate group increased on the 7^th d and decreased on the 9^th and 10^th d. mRNA and protein expression of RIBEYE were initially up-regulated and later down-regulated by injecting salicylate for 10 consecutive days. Conclusion: This change in the ribbon synapses of cochlear inner hair cells in salicylate-induced mice might serve as a compensatory mechanism in the early stages of ototoxicity and contribute to tinnitus later. The alteration of RIBEYE expression could be responsible for the changes in the morphology of ribbon synapses and for salicylate-induced tinnitus.

show abstract

Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 62%

See 1 more Smart Citation

Changes in the Numbers of Ribbon Synapses and Expression of RIBEYE in Salicylate-Induced Tinnitus

Zhang

Xue

Liu

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Cochleas from seven of the aged rats were removed, stored in fixative, and sent to Dr. R. Salvi (Center for Hearing and Deafness, State University of New York, Buffalo, NY), who performed inner-and outer-hair cell counts using previously published methods Spongr et al, 1992). Estimates of inner-and outer-hair cell loss were obtained by comparing hair cell counts from aged FBN rat cochleas to 10 control cochleograms from 10 young-adult animals.…”

Section: Methodsmentioning

confidence: 99%

Age-Related Changes in the Inhibitory Response Properties of Dorsal Cochlear Nucleus Output Neurons: Role of Inhibitory Inputs

Caspary

Schatteman²,

Hughes³

2005

J. Neurosci.

158

114

View full text Add to dashboard Cite

Age-related hearing loss frequently results in a loss in the ability to discriminate speech signals, especially in noise. This is attributable, in part, to a loss in temporal resolving power and ability to adjust dynamic range. Circuits in the adult dorsal cochlear nucleus (DCN) have been shown to preserve signal in background noise. Fusiform cells, major DCN output neurons, receive focused glycinergic inputs from tonotopically aligned vertical cells that also project to the ventral cochlear nucleus. Glycine-mediated inhibition onto fusiform cells results in decreased tone-evoked activity as intensity is increased at frequencies adjacent to characteristic frequency (CF). DCN output is thus shaped by glycinergic inhibition, which can be readily assessed in recordings from fusiform cells. Previous DCN studies suggest an age-related loss of markers for glycinergic neurotransmission. The present study postulated that response properties of aged fusiform cells would show a loss of inhibition, resembling conditions observed with glycine receptor blockade. The functional impact of aging was examined by comparing response properties from units meeting fusiform-cell criteria in young and aged rats. Fusiform cells in aged animals displayed significantly higher maximum discharge rates to CF tones than those recorded from young-adult animals. Fusiform cells of aged rats displayed significantly fewer nonmonotonic CF rate-level functions and an age-related change in temporal response properties. These findings are consistent with an age-related loss of glycinergic input, likely from vertical cells, and with findings from other sensory aging studies suggesting a selective age-related decrement in inhibitory amino acid neurotransmitter function.

show abstract

“…Cochleas from seven of the aged rats were removed, stored in fixative and sent to Dr. Richard Salvi in Buffalo, NY, where inner and outer hair cell counts were performed using previously published methods Spongr et al, 1992). Estimates of inner and outer hair cell loss were obtained by comparing hair cell counts from aged FBN rat cochleas to control cochleograms from 10 youngadult animals.…”

Section: Histologymentioning

confidence: 99%

Age-related changes in the response properties of cartwheel cells in rat dorsal cochlear nucleus

et al. 2006

View full text Add to dashboard Cite

Effects of Noise and Salicylate on Hair Cell Loss in the Chinchilla Cochlea

Cited by 28 publications

References 43 publications

Changes in the Numbers of Ribbon Synapses and Expression of RIBEYE in Salicylate-Induced Tinnitus

Changes in the Numbers of Ribbon Synapses and Expression of RIBEYE in Salicylate-Induced Tinnitus

Age-Related Changes in the Inhibitory Response Properties of Dorsal Cochlear Nucleus Output Neurons: Role of Inhibitory Inputs

Age-related changes in the response properties of cartwheel cells in rat dorsal cochlear nucleus

Contact Info

Product

Resources

About