Effects of NMDA receptor antagonists on olfactory learning and memory in the honeybee (Apis mellifera)

Si, Aung; Helliwell, Paul; Maleszka, Ryszard

doi:10.1016/j.pbb.2003.09.023

Cited by 71 publications

(55 citation statements)

References 37 publications

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“…Jaffe and Blanco (1994) reported an impairment in learning and long-term memory regarding operant conditioning in crickets when the NMDA receptor antagonist APV was injected before training. Maleszka et al (2000) and Si et al (2004) combined the use of glutamate reuptake blockers and NMDAR antagonists in bees and suggested that glutamate plays a role in long-term memory recall. Lopatina et al (2002) tested agonists and antagonists of ionotropic glutamate receptors in honeybees and reported a modulation of memory 1 min after training and no effect 3 h later.…”

Section: Discussionmentioning

confidence: 99%

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Focal and Temporal Release of Glutamate in the Mushroom Bodies Improves Olfactory Memory inApis mellifera

Locatelli

Bundrock²,

Müller³

2005

J. Neurosci.

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In contrast to vertebrates, the role of the neurotransmitter glutamate in learning and memory in insects has hardly been investigated. The reason is that a pharmacological characterization of insect glutamate receptors is still missing; furthermore, it is difficult to locally restrict pharmacological interventions. In this study, we overcome these problems by using locally and temporally defined photo-uncaging of glutamate to study its role in olfactory learning and memory formation in the honeybee, Apis mellifera. Uncaging glutamate in the mushroom bodies immediately after a weak training protocol induced a higher memory rate 2 d after training, mimicking the effect of a strong training protocol. Glutamate release before training does not facilitate memory formation, suggesting that glutamate mediates processes triggered by training and required for memory formation. Uncaging glutamate in the antennal lobes shows no effect on memory formation. These results provide the first direct evidence for a temporally and locally restricted function of glutamate in memory formation in honeybees and insects.

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Section: Discussionmentioning

confidence: 99%

“…Hence, previous works aimed at studying the role of glutamate in the insect brain were based on systemic administration of drugs, whose action specificities are described in vertebrates (Jaffe and Blanco, 1994;Lopatina et al, 2002;Si et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Focal and Temporal Release of Glutamate in the Mushroom Bodies Improves Olfactory Memory inApis mellifera

Locatelli

Bundrock²,

Müller³

2005

J. Neurosci.

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“…Morphine hydrochloride (10 mg/mL) was purchased from To facilitate drug administration, as previously reported [12,24] , individual honeybees were placed on ice for anesthetization and secured in thin-walled straws (5 mm in diameter). The honeybee was mounted in the tube with the head and antennae free to move and with the dorsum of the thorax exposed.…”

Section: Drug Administrationmentioning

confidence: 99%

“…Injection of 0.5 μL morphine (5 μg/bee) or saline was carried out using a 1-μL micro-syringe (Ningbo City Zhenhai Glass Instrument Factory, Ningbo, China). Since most previous studies reported that the injection doses of drugs in the honeybees varied from 0.5 to 2 μL for each insect [20,22,[24][25][26][27][28] , we chose a low dose for the morphine injection in this study.…”

Section: Drug Administrationmentioning

confidence: 99%

“…The present study therefore investigated whether morphine affects the processes underlying memory in insects. By using PER differential conditioning, morphine was administrated 30 min prior to training, immediately following training or 30 min prior to testing, to assess its effects on the acquisition, consolidation and retrieval of memory in honeybees [23,24] .…”

Section: Introductionmentioning

confidence: 99%

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Effects of morphine on associative memory and locomotor activity in the honeybee (Apis mellifera)

Chen

Yao

et al. 2013

Neurosci. Bull.

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Morphine can modulate the processes underlying memory in vertebrates. However, studies have shown various modulations by morphine: positive, negative and even neutral. The honeybee is a potential platform for evaluating the effects of drugs, especially addictive drugs, on the nervous system. However, the involvement of morphine in learning and memory in insects or other invertebrates is poorly understood. The current work evaluated whether morphine affects memory acquisition, consolidation and retrieval in honeybees, using the proboscis extension response (PER) paradigm. We demonstrated that morphine treatment (5 μg/bee) before training decreased the percentage of correct PERs and the response latency related to aversive rather than rewarding odors when tested after 1 or 24 h. Morphine treatment after training also caused a decrease in this latency when tested after 24 h. Meanwhile, morphine treatment reduced the ambulation distance when tested after 30 min. Our findings suggest that morphine impairs the acquisition of short-and long-term associative memory and slightly disrupts the consolidation of long-term memory in honeybees. These negative effects cannot be explained by reduced locomotion but by impaired memory associated with aversion.

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NMDA‐like receptors in the nervous system of the crab Neohelice granulata: A neuroanatomical description

Hepp

Carbó-Tano

Pedreira

et al. 2013

J of Comparative Neurology

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N-Methyl-D-aspartate receptors (NMDARs) are involved in learning and memory processes in vertebrates and invertebrates. In Neohelice granulata, NMDARs are involved in the storage of associative memories (see references in text). The aim of this work was to characterize this type of glutamate receptor in Neohelice and to describe its distribution in the central nervous system (CNS). As a first step, a detailed study of the CNS of N. granulata was performed at the neuropil level, with special focus on one of the main structures involved in this type of memory, the supraesophageal ganglion, called central brain. The characterization of the NMDAR was achieved by identifying the essential subunit of these receptors, the NR1-like subunit. The NR1-like signals were found via western blot and immunohistochemistry techniques in each of the major ganglia: the eyestalk ganglia, the central brain, and the thoracic ganglion. Western blots yielded two bands for the crab NR1-like subunit, at ∼88 and ∼84 kDa. This subunit is present in all the major ganglia, and shows a strong localization in synaptosomal membranes. NMDARs are distributed throughout the majority of each ganglion but show prominent signal intensity in some distinguishable neuropils and neurons. This is the first general description of the N. granulata nervous system as a whole and the first study of NMDARs in the CNS of decapods. The preferential localization of the receptor in some neuropils and neurons indicates the presence of possible new targets for memory processing and storage.

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Effects of NMDA receptor antagonists on olfactory learning and memory in the honeybee (Apis mellifera)

Cited by 71 publications

References 37 publications

Focal and Temporal Release of Glutamate in the Mushroom Bodies Improves Olfactory Memory inApis mellifera

Focal and Temporal Release of Glutamate in the Mushroom Bodies Improves Olfactory Memory inApis mellifera

Effects of morphine on associative memory and locomotor activity in the honeybee (Apis mellifera)

NMDA‐like receptors in the nervous system of the crab Neohelice granulata: A neuroanatomical description

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