Nitrogen is an important factor determining grain yield of barley (Hordeum vulgare L.). Optimal rates and application timing can vary according to the environmental conditions under which the crop is grown. For 8 years barley yield, tillering, and leaf-area response to N application rates and timing were studied in southern Spain. The results showed that, under our experimental conditions, the most advantageous N rate for grain yield was 60 kg/ha, either split equally between sowing and tillering, or else with the greater proportion applied at tillering. Nitrogen applied at sowing increased number of tillers per plant, and N applied at tillering favoured tiller survival for later spike formation. In addition, N applied at tillering increased the number of leaves per plant, leaf area per plant, and the leaf area index, the maximum value of which occurred at anthesis.