Creeping bentgrass {Agrostis stolonifera L.) and velvet bentgrass {Agrostis canina L.) are important turfgrass for golf putting greens. Investigations into wear tolerance mechanisms are limited in Agrostis. The objective of this study was to evaluate anatomical, morphological, and physiological characteristics in Agrostis species and cultivars and relate these to wear tolerance. Seven A. sfo/on/'fera and seven A. canina cultivars were evaluated for response to wear. Wear was applied using a grooming brush over two spring and fall periods. Interspecific differences accounted for 98% of the variation in wear tolerance, with velvet bentgrass exhibiting better tolerance. Ten characteristics were measured on greenhouse-grown spaced-plants established from field plots. Characteristics included tiller density, tiller dry weight, tiller water content, leaf width, leaf strength, leaf angle, tiller angle, and cell wäll constituents (total cell wall [TCW], hemicellulose, and lignocellulose). Depending on the year and season, multiple regression analysis showed TCW accounted for 62.8 to 79;9% of the total variation in wear tolerance followed by tiller density accounting for 53.6 to 66.9% and tiller angle accounting for 75.9% of the variation. SR-7200 was lowest in wear tolerance among velvet bentgrass and exhibited lower cell wall components, lower tiller density, and a more decumbent tiller (and leaf) angle, which was consistent with the lower responses observed in A. stolonifera.