Effects of neurotransmitters, gut hormones, and inflammatory mediators on mucus discharge in rat colon

Plaisancié, Pascale; Barcelo, Aline; Moro, F.; Claustre, Jean; Chayvialle, Jean‐Alain; Cuber, Jean‐Claude

doi:10.1152/ajpgi.1998.275.5.g1073

Cited by 63 publications

(73 citation statements)

References 32 publications

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“…Goblet cells secrete mucin via an unregulated constitutive pathway that is dependent on the continuous movement of mucin granules from the Golgi apparatus to the apex of the cell and by a regulated process dependent on the sudden release of mucin from granules. This regulatory process is controlled by a wide variety of stimuli, including nerve activation and inflammatory mediators (6,31,33). In this study, we clearly demonstrated that leptin is one of these stimuli.…”

Section: Discussionsupporting

confidence: 53%

“…The mucin-like glycoprotein content of samples was determined from standard curves prepared from HT29-MTX mucins isolated from 75-cm 2 flasks and purified by ultracentrifugation as described previously (33). The amount of glycoprotein secreted in the incubation medium was expressed as nanograms of mucin-like glycoprotein per 10 6 cells, and results are given as percentages of controls.…”

Section: Animals Male Wistar Rats Weighing 220 -240 G (Charles Rivermentioning

confidence: 99%

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Luminal leptin activates mucin-secreting goblet cells in the large bowel

Plaisancié¹,

Ducroc²,

Homsi³

et al. 2006

American Journal of Physiology-Gastrointestinal and Liver Physi

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Leptin has been suggested to be involved in tissue injury and/or mucosal defence mechanisms. Here, we studied the effects of leptin on colonic mucus secretion and rat mucin 2 (rMuc2) expression. Wistar rats and ob/ ob mice were used. Secretion of mucus was followed in vivo in the rat perfused colon model. Mucus secretion was quantified by ELISA, and rMuc2 mRNA levels were quantified by real-time RT PCR. The effects of leptin alone or in association with protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K) inhibitors on mucin secreted by human mucus-secreting HT29-MTX cells were determined. Leptin was detected in the rat colonic lumen at substantial levels. Luminal perfusion of leptin stimulates mucus-secreting goblet cells in a dose-dependent manner in vivo in the rat. Leptin (10 nmol/l) increased mucus secretion by a factor of 3.5 and doubled rMuc2 mRNA levels in the colonic mucosa. There was no damage to mucosa 24 h after leptin, but the number of stained mucus cells significantly increased. Leptin-deficient ob/ ob mice have abnormally dense mucus-filled goblet cells. In human colonic goblet-like HT29-MTX cells expressing leptin receptors, leptin increased mucin secretion by activating PKC- and PI3K-dependent pathways. This is the first demonstration that leptin, acting from the luminal side, controls the function of mucus-secreting goblet cells. Because the gel layer formed by mucus at the surface of the intestinal epithelium has a barrier function, our data may be relevant physiologically in defence mechanisms of the gastrointestinal tract.

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Section: Discussionsupporting

confidence: 53%

Section: Animals Male Wistar Rats Weighing 220 -240 G (Charles Rivermentioning

confidence: 99%

Luminal leptin activates mucin-secreting goblet cells in the large bowel

Plaisancié¹,

Ducroc²,

Homsi³

et al. 2006

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Mucus secretagogues. Both PGE 2 and 5-HT are reported to stimulate Cl -, HCO 3 -, and mucus secretion (34)(35)(36)(37). Both agonists are endogenous to the intestine and known to be involved in both physiological functions and pathological conditions in the gut.…”

Section: Mucus Stimulationmentioning

confidence: 99%

Normal mouse intestinal mucus release requires cystic fibrosis transmembrane regulator–dependent bicarbonate secretion

García

Yang²,

Quinton³

2009

J. Clin. Invest.

258

239

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The mechanisms underlying mucus-associated pathologies in cystic fibrosis (CF) remain obscure. However, recent studies indicate that CF transmembrane conductance regulator (CFTR) is required for bicarbonate (HCO 3 -) transport and that HCO 3 -is critical for normal mucus formation. We therefore investigated the role of HCO 3 -in mucus secretion using mouse small intestine segments ex vivo. Basal rates of mucus release in the presence or absence of HCO 3 -were similar. However, in the absence of HCO 3 -, mucus release stimulated by either PGE 2 or 5-hydroxytryptamine (5-HT) was approximately half that stimulated by these molecules in the presence of HCO 3 -. Inhibition of HCO 3 -and fluid transport markedly reduced stimulated mucus release. However, neither absence of HCO 3 -nor inhibition of HCO 3 -transport affected fluid secretion rates, indicating that the effect of HCO 3 -removal on mucus release was not due to decreased fluid secretion. In a mouse model of CF (mice homozygous for the most common human CFTR mutation), intestinal mucus release was minimal when stimulated with either PGE 2 or 5-HT in the presence or absence of HCO 3 -. These data suggest that normal mucus release requires concurrent HCO 3 -secretion and that the characteristically aggregated mucus observed in mucin-secreting organs in individuals with CF may be a consequence of defective HCO 3 -transport.

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“…A variety of peptides may also be involved in the regulation of mucus release during inflammatory states (Lamont 1992, Fairweather 1997. Plaisancié et al (1998) provided evidence that rat colonic mucin secretion may be induced by several mediators (vasoactive intestinal peptide, bombesin, and serotonin) from the neuroendocrine and immune systems, including a marked increase in the discharge of colonic mucin from the isolated vascularly perfused rat colon (Plaisancié et al 1998). Dezfuli et al (2000Dezfuli et al ( , 2002bDezfuli et al ( , 2003 showed that the presence of intestinal helminths in the intestine of the brown trout induced alterations in the occurrence and distribution of the components belonging to the host neuroendocrine system.…”

Section: Discussionmentioning

confidence: 99%

Histochemistry of glycoconjugates in mucous cells of Salmo trutta uninfected and naturally parasitized with intestinal helminths

Bosi¹,

Arrighi²,

Giancamillo³

et al. 2005

Dis. Aquat. Org.

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Mucus secreted onto the surface of the intestine forms a physical barrier to invading parasites so that a possible attachment of helminths to the surface is prevented and their expulsion by peristalsis facilitated. In mammals, intestinal parasites induce hyperplasia and hypertrophy of intestine goblet cells and provoke changes in the mucus composition. In fish, this topic has received less attention. In the present investigation, histochemical methods were employed to compose intestinal mucous cell numbers and their glycoconjugate composition were compared by uninfected brown trout Salmo trutta and in S. trutta parasitized with Cyathocephalus truncatus or Pomphorhynchus laevis. When P. laevis was present in the intestine of the brown trout, the total mucous cell number, and the number of mucous cells containing acid or mixed glycoconjugates were significantly enhanced. No significant change in the total mucous cell number was detected in the intestine of fish parasitized with C. truncatus in comparison with uninfected brown trout. A significant increase was observed in the number of both acid (especially sulphated) and mixed glycoconjugates containing mucous cells as well as a significant decrease in the number of neutral glycoconjugates containing mucous cells. When intestinal helminths were present, the thickness of the adherent mucous gel increased. In a limited number of other fish species, the occurrence of gill and intestinal parasites has been reported to increase the mucosal glycoconjugate secretions. Our study is the first quantitative report on the effects of intestinal helminths on the density of mucous cells and mucus composition in a fish species. KEY WORDS: Glycoconjugates · Mucus composition · Mucous cell number · Intestinal parasites · Brown troutResale or republication not permitted without written consent of the publisher

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Effects of neurotransmitters, gut hormones, and inflammatory mediators on mucus discharge in rat colon

Cited by 63 publications

References 32 publications

Luminal leptin activates mucin-secreting goblet cells in the large bowel

Luminal leptin activates mucin-secreting goblet cells in the large bowel

Normal mouse intestinal mucus release requires cystic fibrosis transmembrane regulator–dependent bicarbonate secretion

Histochemistry of glycoconjugates in mucous cells of Salmo trutta uninfected and naturally parasitized with intestinal helminths

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