Effects of neuron autophagy induced by arsenic and fluoride on spatial learning and memory in offspring rats

Zhao, Qiuyi; Pan, Weizhe; Li, Jia; Yu, Shengnan; Liu, Yan; Zhang, Xiaoli; Qu, Ruodi; Zhang, Qian; Li, Ben; Yan, Xiaoyan; Ren, Xuefeng; Qiu, Yulan

doi:10.1016/j.chemosphere.2022.136341

Cited by 7 publications

(7 citation statements)

References 51 publications

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“…These results suggest that ENO2 can reduce autophagosome accumulation and partially restore autophagy flux. Moreover, LAMP2 is a lysosome membrane protein that expresses lysosome number and together with SQSTM1 reflects lysosomal level 27 . As determined by IHC, the aorta of patients with aortic dissection exhibited decreased expression of LAMP2 compared to control subjects (Fig.…”

Section: Resultsmentioning

confidence: 99%

Elevated ENO2 Disrupts VSMCs Homeostasis Facilitating the Development of Aortic Dissection

Cai¹,

Zhang

Lin

et al. 2023

Preprint

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Objective: Aortic dissection (AD) is a dangerous cardiovascular disease. However, its regulatory mechanism remains poorly understood. Autophagy is an important pathway for maintaining cellular homeostasis. Recent studies have shown that glycolysis and autophagy are essential for the development of AD. Elevated glycolysis can promote cells autophagy, but the relationship between the two is unclear. Enolase 2 (ENO2) is a glycolytic enzyme. Here, we investigated whether ENO2 can regulate autophagy in vascular smooth muscle cells (VSMCs) involved in the progression of AD. Approach and Results: The levels of ENO2 and autophagy-related proteins in aortic tissues from AD patients were identified by qRT-PCR, Western blot and IHC. We found that autophagosome clearance was impaired in aortic tissues and positively correlated with elevated ENO2 expression. Furthermore, aortic application of smooth muscle-specific adeno-associated virus (AAV) inhibiting ENO2 expression attenuated the development of AD in mice. in vitro experiments, downregulation of ENO2 partially restored PDGF-BB-induced impairment of autophagic flux, as evidenced by reduced expression of Beclin-1, SQSTM1, LC3BII/I and increased levels of LAMP2 in human aortic vascular smooth muscle cells (HAVSMCs). Similarly, increased levels of ENO2 exacerbated the autophagic dysfunction in HAVSMCs. Mechanistically, ENO2 may block autophagic flux through the ENO2-GAP43-ATF4 pathway and disrupt cellular homeostasis. Conclusion: Our data reveal a perturbing role of ENO2 in the homeostasis of VSMCs, suggesting ENO2 as a potential target for intervention in AD.

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Section: Resultsmentioning

confidence: 99%

Elevated ENO2 Disrupts VSMCs Homeostasis Facilitating the Development of Aortic Dissection

Cai¹,

Zhang

Lin

et al. 2023

Preprint

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“…Arsenic is classified as a human carcinogen, which can lead to many adverse health effects including DM, 31−33 skin lesions, 34,35 kidney disease, 36,37 neurological impairment, 28,38 cancer, 39,40 male reproductive injury, 30 and CVD. 40,41 miRNAs dysregulation was considered to be one of the important mechanisms by which arsenic leads to toxicity and human diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Arsenic is classified as a human carcinogen, which can lead to many adverse health effects including DM, − skin lesions, , kidney disease, , neurological impairment, , cancer, , male reproductive injury, and CVD. , miRNAs dysregulation was considered to be one of the important mechanisms by which arsenic leads to toxicity and human diseases. ,,,, miRNAs can act as tumor suppressors and oncogenes, and the balance between the above two roles determined its carcinogenic potential. The following miRNAs were downregulated by arsenic, including miR-217, miR-182-5p, miR-455, Let-7a/b/c, miR-181b, miR-9, miR-200b/c, miR-410, miR-548ac, miR-3174, miR-138, miR-205, miR-34c-5p, miR-143, miR-181d/c, miR-6733, miR-148a, miR-373, and miR-134, while the majority of miRNAs that function as oncogenes are upregulated by arsenic exposure, such as miR-638, miR-155, miR-21, miR-889, miR-15b, miR-191, miR-186, miR-200a, miR-141, miR-190, miR-330-5p, miR-6734-5p, miR-885-5p, miR-184, miR-576-3p, miR-222, miR-221, and miR-451, which can directly target and inhibit the expression of tumor suppressors of RNF4, TGFβ1, VEGF, PDCD4, PTEN, Spry1, DAB2IP, LATS1, BASP1, BUB1, ACVR2A, CDK6, BMPR2, PHLPP, Skp2, IDH2, and MTPN.…”

Section: Discussionmentioning

confidence: 99%

“…24 A growing body of literature suggests that arsenic disrupts a series of cellular processes, including oxidative stress, 25 cell proliferation, 26 inflammation, 27 and autophagy and apoptosis. 25,28−30 Arsenic exposure can lead to many adverse health effects of diabetes mellitus (DM), 31−33 skin lesions, 34,35 kidney disease, 36,37 neurological impairment, 28,38 cancers, 39,40 male reproductive injury, 30 and cardiovascular disease (CVD) as cardiac arrhythmias, endothelial dysfunction, and ischemic heart failure. 40,41 miRNAs are involved in pathogenic mechanisms and regulated by oxidative stress and inflammation upon exposure to heavy metals.…”

Section: Introductionmentioning

confidence: 99%

“…More than 50 million people from West Bengal and Bangladesh were exposed to arsenic levels above 50 μg/L, which caused frequent arsenic poisoning and adverse effects on human health . A growing body of literature suggests that arsenic disrupts a series of cellular processes, including oxidative stress, cell proliferation, inflammation, and autophagy and apoptosis. ,− Arsenic exposure can lead to many adverse health effects of diabetes mellitus (DM), − skin lesions, , kidney disease, , neurological impairment, , cancers, , male reproductive injury, and cardiovascular disease (CVD) as cardiac arrhythmias, endothelial dysfunction, and ischemic heart failure. , …”

Section: Introductionmentioning

confidence: 99%

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The Role of microRNAs in Arsenic-Induced Human Diseases: A Review

Liu,

Lei

2023

J. Agric. Food Chem.

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MicroRNAs (miRNAs) are noncoding RNAs with 20–22 nucleotides, which are encoded by endogenous genes and are capable of targeting the majority of human mRNAs. Arsenic is regarded as a human carcinogen, which can lead to many adverse health effects including diabetes, skin lesions, kidney disease, neurological impairment, male reproductive injury, and cardiovascular disease (CVD) such as cardiac arrhythmias, ischemic heart failure, and endothelial dysfunction. miRNAs can act as tumor suppressors and oncogenes via directly targeting oncogenes or tumor suppressors. Recently, miRNA dysregulation was considered to be an important mechanism of arsenic-induced human diseases and a potential biomarker to predict the diseases caused by arsenic exposure. Endogenic miRNAs such as miR-21, the miR-200 family, miR-155, and the let-7 family are involved in arsenic-induced human disease by inducing translational repression or RNA degradation and influencing multiple pathways, including mTOR/Arg 1, HIF-1α/VEGF, AKT, c-Myc, MAPK, Wnt, and PI3K pathways. Additionally, exogenous miRNAs derived from plants, such as miR-34a, miR-159, miR-2911, miR-159a, miR-156c, miR-168, etc., among others, can be transported from blood to specific tissue/organ systems in vivo. These exogenous miRNAs might be critical players in the treatment of human diseases by regulating host gene expression. This review summarizes the regulatory mechanisms of miRNAs in arsenic-induced human diseases, including cancers, CVD, and other human diseases. These special miRNAs could serve as potential biomarkers in the management and treatment of human diseases linked to arsenic exposure. Finally, the protective action of exogenous miRNAs, including antitumor, anti-inflammatory, anti-CVD, antioxidant stress, and antivirus are described.

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Sparassis latifolia polysaccharide alleviated lipid metabolism abnormalities in kidney of lead-exposed mice by regulating oxidative stress-mediated inflammation and autophagy based on multi-omics

Lu,

Wu,

et al. 2024

International Journal of Biological Macromolecules

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Effects of neuron autophagy induced by arsenic and fluoride on spatial learning and memory in offspring rats

Cited by 7 publications

References 51 publications

Elevated ENO2 Disrupts VSMCs Homeostasis Facilitating the Development of Aortic Dissection

Elevated ENO2 Disrupts VSMCs Homeostasis Facilitating the Development of Aortic Dissection

The Role of microRNAs in Arsenic-Induced Human Diseases: A Review

Sparassis latifolia polysaccharide alleviated lipid metabolism abnormalities in kidney of lead-exposed mice by regulating oxidative stress-mediated inflammation and autophagy based on multi-omics

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