Effects of Nerve Growth Factor and Basic Fibroblast Growth Factor Promote Human Dental Pulp Stem Cells to Neural Differentiation

Abstract

Dental pulp stem cells (DPSCs) were the most widely used seed cells in the field of neural regeneration and bone tissue engineering, due to their easily isolation, lack of ethical controversy, low immunogenicity and low rates of transplantation rejection. The purpose of this study was to investigate the role of basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) on neural differentiation of DPSCs in vitro. DPSCs were cultured in neural differentiation medium containing NGF and bFGF alone or combination for 7 days. Then neural genes and protein markers were analyzed using western blot and RT-PCR. Our study revealed that bFGF and NGF increased neural differentiation of DPSCs synergistically, compared with bFGF and NGF alone. The levels of Nestin, MAP-2, βIII-tubulin and GFAP were the most highest in the DPSCs + bFGF + NGF group. Our results suggested that bFGF and NGF signifiantly up-regulated the levels of Sirt1. After treatment with Sirt1 inhibitor, western blot, RT-PCR and immunofluorescence staining showed that neural genes and protein markers had markedly decreased. Additionally, the ERK and AKT signaling pathway played a key role in the neural differentiation of DPSCs stimulated with bFGF + NGF. These results suggested that manipulation of the ERK and AKT signaling pathway may be associated with the differentiation of bFGF and NGF treated DPSCs. Our date provided theoretical basis for DPSCs to treat neurological diseases and repair neuronal damage.

Heparin-Poloxamer Thermosensitive Hydrogel Loaded with bFGF and NGF Enhances Peripheral Nerve Regeneration in Diabetic Rats

“…The individual effect of bFGF and NGF has been validated in our prior studies [33,84]. Studies have indicated that co-administration of NGF and bFGF together could synergistically promote tissue reinnervation and neural differentiation, compared with bFGF and NGF alone [85,86]. In this study, we evaluated the effectiveness of dual GF administration for the first time in peripheral nerve injuries, and focused on showing how HP hydrogel can augment this therapy strategy.…”

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

“…Dental pulp MSCs demonstrate immunomodulatory properties partly attributable to their expression of IL-8, IL-6, and TGF-β, which could inhibit T cell function [139,140]. Moreover, dental pulp MSCs secrete many neurotrophic factors like BDNF [141], glial cell linederived neurotrophic factor [142], and NGF [143]. Although dental pulp MSCs and SHED originate from dental pulpal tissues and share many common properties, SHED demonstrated a higher proliferation rate but lower osteogenic potential as compared to dental pulp MSCs [144].…”

Paraquat affects the differentiation of neural stem cells and impairs the function of vascular endothelial cells: a study of molecular mechanism

“…Studies have confirmed that VEGF and BFGF can promote the proliferation, differentiation, and migration of embryonic neural stem cells by activating transcription factors, and have direct neuroprotective and neurotrophic effects on neurons and glial cells . In addition, Hawkins et al found that vascular endothelial cells also form a natural barrier that selectively blocks the invasion of exogenous substances while delivers nutrients to neural stem cells .…”