“…The synergistic effects of two irradiation wavelengths (23,24) and of light and heat (21) and the action of light on bacteria and living cells have been well documented (11)(12)(13). The most effective wavelengths for microbial destruction are the near-UV-A band (320 to 400 nm) and to a lesser extent the visible band of violet and blue light, 400 to 490 nm (2,21).…”
Contaminated water causes an estimated 6 to 60 billion cases of gastrointestinal illness annually. The majority of these cases occur in rural areas of developing nations where the water supply remains polluted and adequate sanitation is unavailable. A portable, low-cost, and low-maintenance solar unit to disinfect unpotable water has been designed and tested. The solar disinfection unit was tested with both river water and partially processed water from two wastewater treatment plants. In less than 30 min in midday sunlight, the unit eradicated more than 4 log 10 U (99.99%) of bacteria contained in highly contaminated water samples. The solar disinfection unit has been field tested by Centro Panamericano de Ingenieria Sanitaria y Ciencias del Ambiente in Lima, Peru. At moderate light intensity, the solar disinfection unit was capable of reducing the bacterial load in a controlled contaminated water sample by 4 log 10 U and disinfected approximately 1 liter of water in 30 min.
“…The synergistic effects of two irradiation wavelengths (23,24) and of light and heat (21) and the action of light on bacteria and living cells have been well documented (11)(12)(13). The most effective wavelengths for microbial destruction are the near-UV-A band (320 to 400 nm) and to a lesser extent the visible band of violet and blue light, 400 to 490 nm (2,21).…”
Contaminated water causes an estimated 6 to 60 billion cases of gastrointestinal illness annually. The majority of these cases occur in rural areas of developing nations where the water supply remains polluted and adequate sanitation is unavailable. A portable, low-cost, and low-maintenance solar unit to disinfect unpotable water has been designed and tested. The solar disinfection unit was tested with both river water and partially processed water from two wastewater treatment plants. In less than 30 min in midday sunlight, the unit eradicated more than 4 log 10 U (99.99%) of bacteria contained in highly contaminated water samples. The solar disinfection unit has been field tested by Centro Panamericano de Ingenieria Sanitaria y Ciencias del Ambiente in Lima, Peru. At moderate light intensity, the solar disinfection unit was capable of reducing the bacterial load in a controlled contaminated water sample by 4 log 10 U and disinfected approximately 1 liter of water in 30 min.
“…Previous studies have shown that the 3 2 W nm (UV-A) region produces lethal, inhibitory, and delaying effects (Doyle and Kubitschek, 1976; Elkind el a/.. Fong er al.. : Jagger. 1975. 1976Koch et al. 1976;Webb.…”
We have investigated the influence of narrow-band UV radiation. 265-330 nm. on germination of spores of the fungus Cladosporium cucumrrinum Ellis and Arth.. using a Xe arc lamp and filters. Reciprocity of time and dose rate was demonstrated when fungal spores were subjected to UV radiation at 325 nm but failed to hold at 265 nm. Based on these findings, data on fluence response. and partial action spectra. we propose that there are two biologically active sites in this organism that are affected by radiation between 265 and 330nm and that might be influenced by changes in the stratospheric ozone layer: a short-wave-sensitive site (265--295 nm) and a long-wave-sensitive site (300 330 nm). Data obtained with narrow-band interference filters confirmed previous reports of damage to nucleic acid from UV at 265-295nm and in addition demonstrated significant inhibition by UV at 3 W 3 2 0 n m . Further studies of the 300 330nm portion of the spectrum. using combinations of plastic and glass filters. showed that the influence of IJV radiation in this region was primarily to produce a nonphotoreactivable delay in germ-tube emergence.
“…Oral 8-methoxypsoralen (8-MOP) plus high intensity long wavelength ultraviolet radiation (UV-A) is presently being used experimentally to induce remissions of psoriasis (Lak Shimpsthi et Melski et al, 1977;Parrish et al, 1974;Swanbeck et al, 1974;Wolff et al, 1976) and in the cutaneous lymphoma, mycosis fungoides (Gilchrist et al., 1976;Hodge et al, 1977;Roenigk, 1977). This treatment is used to decrease the rate of proliferation of cells in the skin of patients with these diseases.…”
Abstract— 8‐Methoxypsoralen (8‐MOP) plus high intensity long wavelength ultraviolet radiation (UV‐A) is presently being used to induce remissions of psoriasis and mycosis fungoides. Previous studies demonstrated inhibition of DNA synthesis in circulating leukocytes from some patients during this therapy. The present study is designed to determine whether conditions of 8‐MOP concentration and UV‐A exposure attained during therapy might be sufficient to result directly in decreased lymphoid cell DNA synthesis and viability in vitro. Tritiated thymidine (3HTdR) incorporation and cell growth in suspension culture following UV‐A exposure alone or with therapeutic concentrations of 8‐MOP was examined in peripheral blood lymphocytes and in Ebstein‐Barr virus transformed human lymphoblas‐toid cell lines. UV‐A exposure alone induced a dose‐dependent inhibition of HTdR incorporation in both types of lymphoid cells (3000 J/m2 resulted in 77% of control 3HTdR incorporation). Pre‐incubation with 0.1 μg/m/ 8‐MOP before UV‐A exposure induced a significantly greater inhibition of 3HTdR incorporation (3000 J/m2 resulted in 61% of control 3HTdR incorporation). Greater inhibition of 3HTdR incorporation was observed by preincubation of the lymphoblastoid cells with 1.0μg/mC 8‐MOP (3000 J/m2 resulted in 41% of control) but not in the lymphocytes (3000 J/m2 resulted in 63% of control). The concentration of viable lymphoblastoid cells did not decrease below the original concentration after the highest dose of UV‐A alone (29,000 J/m2) but preincubation with 0.1 μg/mC 8‐MOP resulted in 40% survival after 3000 J/m2 (D37 approximately 3000 J/m2) and preincubation with 1.0 μg/ 8‐MOP resulted in 0.6% survival after 3000 J/,2 (D37 approximately 800 J/m2). This study suggests that the low doses of 8‐MOP and UV‐A received by patients' lymphocytes may be sufficient to explain the decreased DNA synthesis found in their circulating leucocytes. The long term consequences of such damage remain to be determined.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.