Effects of Nd:Yag Laser on some virulence factor genes of Pseudomonas aeruginosa bacteria

Abstract

The aim of this study was to assess effects of the 532nm Nd-yag laser on the genes of Tox A, Exo S, and Opr L, of Pseudomonas aeruginosa (P. aeruginosa) bacteria isolated from clinical (wounds, burns, otitis media) and environmental (water, soil) samples. Clinical samples were collected from patients coming to Saladdin General Hospital from wound, burns and middle ear infections while environmental samples were extracted from water and soil for Saladdin General Hospital . Bacterial samples irradiated by Nd-Yag laser with wavelength of 532 nm using energies (300mj,500mj) with (15 and 25 sec) and genomic DNA were extracted from all samples after the diagnosis of P. aeruginosa bacteria depending on the macroscopic and biochemical examination, then the PCR technique was performed. The results have shown an impact on P. aeruginosa bacteria of Nd-Yag laser by comparing PCR results of treated samples with control (unexposed) as loss of normal bands. This indicates that the laser had a genetic effect on the P. aeruginosa bacteria. We conclude that the laser induces genetic changes in P. aeruginosa's DNA so that lasers can be used in treatment and sterilization for clinical and environmental . The PCR technique could be used as a biomarker study to determine the biological effects of radiation on bacteria.
 
 http://dx.doi.org/10.25130/tjps.25.2020.034

Prevalence, Antibiotics Susceptibility Profile of <i>Listeria Monocytogenes</i>, and Its Associated Risk Factors Among Pregnant Women Attending Millennium Health Center

“…In this study, the prevalence of L. monocytogenes among pregnant women was found to be higher in the age group 25-34 years (8/72; 11.11%) with AOR=4.40 (95% CI=0.61-31.74), p=0.141), agreement with findings [21,33], daily worker (4/40; 10%) with COR=2.89 (95% CI=0.50-16.62), p=0.235), and rural (3/39; 7.69%) with COR=1.63 (95% CI=0.41-6.43), p=0.489), agreement with findings [34], third trimester (6/77; 7.79%) with COR=3.38 (95% CI=0.39-29.08), p=0.267), agreement with findings [33,6]. The possible reasons for the high prevalence in the present study could be the high-risk group, populated area, pregnancyrelated suppressed cell-mediated immunity, placental tropism of L. monocytogenes and sudden changes in hormones during pregnancy.…”

“…However, it was greater than the study findings in Arash Hospital in Tehran, Iran (4, 4.0%) [31] and a study conducted on fecal carriers in HIV-uninfected pregnant women in Brazil (2.9%) [32]. On the other hand, it was less than the findings of the Gynecology Department, Bharatpur, Nepal (16.7%) [33], Salah Aldeen Province (13.82%) [21], Tigray region, Northern Ethiopia (8.5%) [34], and India (6.3%) [35]. The variation in the study findings might be due to differences in sociodemographic characteristics, study population, access to health facilities, and methodology used.…”

“…It survives and replicates over a wide range of temperatures (4 -42°C), pH, salt, and oxygen concentrations [17] and forms biofilms, which makes elimination difficult [18]. It can be isolated from maternal/neonatal blood, body fluid, and the placenta [19] by growing on different culture media [20,21]. A mixture of ampicillin and aminoglycoside, ampicillin and gentamicin is the current therapy of choice [22] despite the emergence of drug-resistant strains [23].…”