Effects of nature, size and level of incorporation of dietary fibres on colonic functions in germ-free rats and in heteroxenic rats inoculated with a human flora

Dufour-Lescoat, C.; Coz, Y. Le; Andrieux, C.P.; Szylit, Odette

doi:10.1016/s0268-005x(09)80189-6

“…For this parameter, the results were close to those obtained with partially soluble übres such as carrot, cocoa (Roland et al 1995) and beet übres (Dufour-Lescoat et al 1995). In humans, methane production was associated with a slow gut transit time and a high intestinal pH (Stephen et al 1986).…”

Section: Discussionsupporting

confidence: 78%

“…Previous studies with germ-free rats and rats associated with human faecal ýora fed diþerent übres or poorly digestible carbohydrates, have shown that the ability to produce organic acids and gas was maintained when human ýora was inoculated into germ-free rats (Andrieux et al 1991 ;Dufour-Lescoat et al 1995 ;Roland et al 1995). Fermentation metabolites varied with the origin of the partially soluble übres, cocoa, beet übre, carrot and wheat bran, as far as SCFA (Roland et al 1995) and gas production (DufourLescoat et al 1995 ;Roland et al 1995) are concerned.…”

Section: Introductionmentioning

confidence: 99%

Ulva lactuca is poorly fermented but alters bacterial metabolism in rats inoculated with human faecal flora from methane and non-methane producers

Andrieux

¹

,

Hibert

²

,

Houari

³

et al. 1998

J. Sci. Food Agric.

View full text Add to dashboard Cite

In vivo fermentation of Ulva lactuca was studied in previously germ‐free rats inoculated with human flora obtained from non‐, low‐ and high‐methane producers (groups NMP, LMP and HMP, respectively), in comparison to germ‐free rats. Rats were fed either a control diet or a diet containing 4% of dried Ulva lactuca. Production of metabolites varied according to the flora and diet. Ulva lactuca induced a specific high production of methane in the HMP group. With the three human flora, Ulva diet induced a similar increase in caecal pH. In the NMP and HMP groups, this increase was associated with a fall of lactic acid caecal concentration. In the LMP group it was related to a decrease in the concentration of short‐chain fatty acids. Ulva lactuca appeared to be able to regulate the β‐glucuronidase and β‐glucosidase activities, reducing the relatively high levels observed in groups NMP and HMP and increasing the low levels obtained in the LMP group. Results show that, although it was poorly fermented, Ulva induced significant effects on the gut microflora metabolism. The methanogenic status of the human donor appeared to be an important factor. © 1998 SCI.

show abstract

“…for four rats. Similar effects have been observed in man during chronic lactulose ingestion (Florent et al 1985), although rats given beet fibre continued to produce large amounts of methane (Dufour-Lescoat et al 1995). which has also been reported to occur with dietary fibre (Nyman and Asp 1982) and other poorly digestible carbohydrates (Andrieux et al 1989).…”

Section: Discussionsupporting

confidence: 52%

“…Irrespective of its dietary concentration, TOS caused an increase in hydrogen excretion, while methane production was not significantly modified. Similar effects have been observed in man during chronic lactulose ingestion (Florent et al 1985), although rats given beet fibre continued to produce large amounts of methane (Dufour-Lescoat et al 1995).…”

Section: Discussionmentioning

confidence: 54%

Effect of two levels of transgalactosylated oligosaccharide intake in rats associated with human faecal microflora on bacterial glycolytic activity, end‐products of fermentation and bacterial steroid transformation

Kikuchi¹,

Andrieux²,

Riottot³

et al. 1996

Journal of Applied Bacteriology

Self Cite

View full text Add to dashboard Cite

The effects of two levels of transgalactosylated oligosaccharide (TOS) intake on bacterial glycolytic activity, end products of fermentation and bacterial steroid transformation were studied in rats associated with a human faecal flora. Rats were fed a human-type diet containing 0, 5 or 10% TOS. Caecal pH decrease correlated with the amount of TOS in the diet. Intake of the TOS diet induced a decrease in blood cholesterol and a strong increase in beta-galactosidase activity in the hindgut. TOS fermentation led to production of hydrogen and short chain fatty acids, whereas ammonia and branched-chain fatty acids were decreased. A diet containing 10% TOS increased caecal lactic acid concentrations and reduced beta-glucuronidase activities and steroid transformation.

show abstract

“…Previous studies with germ-free rats and rats associated with human faecal ýora fed diþerent übres or poorly digestible carbohydrates, have shown that the ability to produce organic acids and gas was maintained when human ýora was inoculated into germ-free rats (Andrieux et al 1991 ;Dufour-Lescoat et al 1995 ;Roland et al 1995). Fermentation metabolites varied with the origin of the partially soluble übres, cocoa, beet übre, carrot and wheat bran, as far as SCFA (Roland et al 1995) and gas production (Dufour-Lescoat et al 1995 ;Roland et al 1995) are concerned. Moreover, the methanogenic status inýuences the metabolite proüle.…”

Section: Introductionmentioning

confidence: 99%

Ulva lactuca is poorly fermented but alters bacterial metabolism in rats inoculated with human faecal flora from methane and non‐methane producers

Andrieux¹,

Hibert²,

Houari³

et al. 1998

J. Sci. Food Agric.

View full text Add to dashboard Cite

In vivo fermentation of Ulva lactuca was studied in previously germ‐free rats inoculated with human flora obtained from non‐, low‐ and high‐methane producers (groups NMP, LMP and HMP, respectively), in comparison to germ‐free rats. Rats were fed either a control diet or a diet containing 4% of dried Ulva lactuca. Production of metabolites varied according to the flora and diet. Ulva lactuca induced a specific high production of methane in the HMP group. With the three human flora, Ulva diet induced a similar increase in caecal pH. In the NMP and HMP groups, this increase was associated with a fall of lactic acid caecal concentration. In the LMP group it was related to a decrease in the concentration of short‐chain fatty acids. Ulva lactuca appeared to be able to regulate the β‐glucuronidase and β‐glucosidase activities, reducing the relatively high levels observed in groups NMP and HMP and increasing the low levels obtained in the LMP group. Results show that, although it was poorly fermented, Ulva induced significant effects on the gut microflora metabolism. The methanogenic status of the human donor appeared to be an important factor. © 1998 SCI.

show abstract

Effects of nature, size and level of incorporation of dietary fibres on colonic functions in germ-free rats and in heteroxenic rats inoculated with a human flora

Cited by 6 publications

References 23 publications

Ulva lactuca is poorly fermented but alters bacterial metabolism in rats inoculated with human faecal flora from methane and non-methane producers

Ulva lactuca is poorly fermented but alters bacterial metabolism in rats inoculated with human faecal flora from methane and non-methane producers

Effect of two levels of transgalactosylated oligosaccharide intake in rats associated with human faecal microflora on bacterial glycolytic activity, end‐products of fermentation and bacterial steroid transformation

Ulva lactuca is poorly fermented but alters bacterial metabolism in rats inoculated with human faecal flora from methane and non‐methane producers

Contact Info

Product

Resources

About