Effects of naturally-occurring flavonoids and biflavonoids on epidermal cyclooxygenase and lipoxygenase from guinea-pigs

Kim, H.P.; Mani, Indu; Iversen, Lars; Ziboh, Vincent A.

doi:10.1016/s0952-3278(98)90125-9

Cited by 277 publications

(160 citation statements)

References 21 publications

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“…While there was no evidence of modulation of cyclooxygenase activity during this human intervention trial there have been reports of altered cyclooxygenase and lipoxygenase activities in various in vitro animal or cell culture systems Laughton et al, 1991;Kim et al, 1998). This may re¯ect the signi®cantly higher¯avo-noid concentrations used in in vitro studies (10 ± 50 mM; Mower et al, 1984) compared with those achieved following 6 weeks' rutin supplementation (mean plasma quercetin concentration 0.14 + 0.03 mmolal plasma).…”

Section: Discussionmentioning

confidence: 82%

Bioavailability and efficiency of rutin as an antioxidant: a human supplementation study

Boyle¹,

Dobson²,

Duthie³

et al. 2000

Eur J Clin Nutr

179

107

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Objective: To determine the potential antioxidant effect of rutin (quercetin-3-O-b-rutinoside) supplementation. Design: A 6-week randomized single-blind placebo controlled trial was conducted; 500 mg rutin supplement was compared to an equivalent amount of glucose placebo. In addition, a pharmacokinetic study was carried out. Setting: The Rowett Research Institute, Aberdeen, UK. Subjects: Eighteen healthy non-obese normocholesterolaemic female volunteers in the age range 18 ± 48 y. Main outcome measures: Plasma¯avonoids, ascorbic acid, tocopherols and carotenoids, plasma antioxidant capacity, lymphocyte DNA damage, blood chemistry and haematology, liver function tests, urinary malondialdehyde, 8-hydroxy-2H -deoxyguanosine and 8-iso-prostaglandin F 2a . Results: Eighteen volunteers completed the trial. Rutin supplementation did not induce any adverse changes in blood chemistry or indices of liver function. Plasma¯avonoids were signi®cantly elevated in the rutinsupplemented group. Endogenous oxidation of pyrimidines was signi®cantly decreased in both rutin-and placebo-treated volunteers. There was no signi®cant change in the level of urinary 8-hydroxy-2 H -deoxyguanosine or urinary malondialdehyde in either group. A linear correlation was observed between urinary malondialdehyde and urinary 8-iso-prostaglandin F 2a (R 0.54, P`0.01). Conclusion: Six weeks' rutin supplementation signi®cantly elevated the levels of three plasma¯avonoids (quercetin, kaempferol and isorhamnetin) but there was no signi®cant change in plasma antioxidant status. The decrease in the level of endogenous base oxidation in lymphocyte DNA seen in both the placebo-and rutinsupplemented subjects may re¯ect seasonal changes in other dietary antioxidants.

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Section: Discussionmentioning

confidence: 82%

Bioavailability and efficiency of rutin as an antioxidant: a human supplementation study

Boyle¹,

Dobson²,

Duthie³

et al. 2000

Eur J Clin Nutr

179

107

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“…Flavanoid olan hiperosid ve izokuarsitrin inflamasyon patogenezinde rol alan nötrofil elastazı inhibe ettiği, hiperosid nitrik oksit sentazı inhibe ederken izokuarsitrin'in de prostoglandin biyosentezini ve salınımını inhibe ettiği gösterilmiştir (29,30). Buna ilaveten flavanoid olan amentoflanon'un siklooksijenaz-2, fosfolipaz A2, iNOS'u ve nötrofillerden araşidonik asit salınımını ingibe ettiği gösterimiştir (31)(32)(33)(34)(35)(36).…”

Section: Discussionunclassified

Kantaron Ekstresinin Gentamisinin Neden Oldugu Ototoksisite Uzerinde Koruyucu Etkisi

2017

GMJ

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“…Radiolabeled cells were then incubated separately with increasing concentrations of T or DHT and their respective effects on the biosynthesis of radiolabeled lipoxygenase monohydroxy fatty acids determined. The 15-LOX-derived 14 C-15S-HETE and the 5-LOX-derived 14 C-5S-HETE were the 2 major LOX metabolites generated by these cells. Because AA metabolism in BHCs was minor (data not shown) compared to MTCs, we focused our subsequent studies on the androgenic effects in tumorigenic cells (MTCs).…”

Section: Androgen Effect On the Generation Of Lox Monohydroxy Fatty Amentioning

confidence: 92%

“…Fatty acid methyl esters were detected with a flame ionization detector set at 250°C and quantitated by the heptadecanoate (17:0) internal standard, as previously reported. 13 Metabolism of 14 14 C-AAs) were subjected to TLC and developed in the solvent system of ethyl acetate:acetic acid (99:1, v/v). Prior to TLC, nonradioactive prostanoids (PGE 1 , PGE 2 , PGD 2 , PGF 2␣ ) and LOX standards (5S-HETE, 12S-HETE and 15S-HETE) were added as standards to the extracted 14 C metabolites for identification.…”

Section: Determination Of Phospholipid Fatty Acid Compositionmentioning

confidence: 99%

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Suppression of cyclooxygenase‐2 overexpression by 15S‐hydroxyeicosatrienoic acid in androgen‐dependent prostatic adenocarcinoma cells

Pham

Banerjee

Ziboh

2004

Intl Journal of Cancer

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PGs and monohydroxy fatty acids (products of AA oxygenation), collectively known as "eicosanoids", are constitutive modulators in the prostate gland. 1 The precursor AA (20:4n-6) is converted to 2-series PGs via the COX pathway and to monohydroxy fatty acids and leukotrienes via the LOX pathway. These metabolites have been reported in other systems to modulate downstream physiologic and pathophysiologic responses such as growth and metastases. 2,3 In addition to eicosanoids, the androgens, specifically T (⌬ 4 -androstene-17␤-ol-3-one) and its reduced metabolite DHT (5␣-androstan-17␤-ol-3-one), are constitutive modulators in the prostate gland. 4 In hormone-responsive tissue such as the prostate, T is converted to the biologically active metabolite DHT by the enzyme 5␣-reductase, thus making this enzyme key to hormonal regulation of the prostate gland. 5-7 DHT in turn binds to a nuclear receptor, which regulates cellular growth and gene expression. 8 -10 Despite this latter function of DHT, the interactions and effects of androgens and AA metabolites on the pathophysiology of prostate cancer have remained obscure. However, one in vitro study using androgen-responsive prostate cancer cells suggested that a relationship exists between androgens and lipid metabolism.11 Because eicosanoids are important biologic modulators in the pathogenesis of cancer, we designed this study to test the hypothesis that T and its in vivo metabolite (DHT) alter the metabolism of AA in BHCs and tumorigenic prostatic MTCs in culture and that the alteration in eicosanoid production may contribute, at least in part, to tumorigenesis in prostate cells. Thus, using cells derived from the Lobund-Wistar rat model of autochthonous prostate adenocarcinoma, we tested in vitro whether or not preincubation of cells with GLA, a dietary constituent of borage oil, and its 15-LOX metabolite (15S-HETrE) would suppress the growth of androgen-responsive prostate cancer cells. MATERIAL AND METHODS Propagation of cellsWe used the established and well-characterized prostatic BHC line NRP-152 and the malignant tumorigenic line NRP-154, which were generously provided by Dr. D. Danielpour (Ireland Cancer Center, Case Western Reserve University, Cleveland, OH). These cells were propagated as previously reported. 12 Briefly, both NRP-152 (derived from the prostate of Lobund-Wistar rats that had been previously treated only with N-nitroso-N-methylurea) and NRP-154 (derived from the prostate of Lobund-Wistar rats previously treated with N-nitroso-N-methylurea plus T) cells were maintained in GM2 [HEPES-free and antibiotic-free DMEM/F-12 (1:1, v/v) supplemented with 5% FBS, 20 ng/ml EGF, 10 ng/ml cholera toxin, 0.1 M dexamethasone and 5 g/ml insulin] in 80 cm 2

show abstract

Effects of naturally-occurring flavonoids and biflavonoids on epidermal cyclooxygenase and lipoxygenase from guinea-pigs

Cited by 277 publications

References 21 publications

Bioavailability and efficiency of rutin as an antioxidant: a human supplementation study

Bioavailability and efficiency of rutin as an antioxidant: a human supplementation study

Kantaron Ekstresinin Gentamisinin Neden Oldugu Ototoksisite Uzerinde Koruyucu Etkisi

Suppression of cyclooxygenase‐2 overexpression by 15S‐hydroxyeicosatrienoic acid in androgen‐dependent prostatic adenocarcinoma cells

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