Effects of natural environment on reproductive histo-morphometric dynamics of female dromedary camel

Ali, Hafız Muhammad; Qureshi, Anas Sarwar; Hussain, Riaz; Urbinati, Giorgia; Mustafa, Mohammad Zahid; Ali, Farah; Manan, Abdul; Massaad-Massade, Liliane

doi:10.1016/j.anireprosci.2017.03.012

Cited by 11 publications

(9 citation statements)

References 47 publications

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“…A significant increase in number ( p < .05) and size ( p < .05) of surface with secondary and tertiary ovarian follicles in winter season was observed (Ali et al., ). They concluded that quiescent ovarian follicular and uterine glandular activities are the main reason of camel low breeding during summer season.…”

Section: Discussionmentioning

confidence: 99%

Histological and immunohistochemical investigations of ovarian interstitial glands during non‐breeding season in camels (Camelus dromedarius)

Mohamed

Amin

Hussein

2018

Reprod Domestic Animals

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The aim of this was to investigate the histology and immunohistochemistry of interstitial glands during non-breeding season in camel ovaries. A total of 21 mature, non-pregnant and apparently healthy camels aged between 8 and 12 years were slaughtered. The ovaries were removed within 15 min, cleaned from adipose tissue, weighted and examined grossly. The histological preparation was made, and then, the blocks were cut at 3-5 microns thickness and stained by H&E for histological examinations. Moreover, some sections were stained with Sudan Black for lipid detection. Immunohistochemical analysis of paraffin-embedded ovarian tissues was performed to detect the localization of S-100, vimentin, progesterone receptors (PR) and oestrogen receptors (ER). Immunoreactive signals were detected using UltraVision Detection System. The results revealed that the interstitial glands were located in the cortical region and they were arranged in various arrangements either single, in couple or in groups rich in lipid droplet. All interstitial gland arrangements were enclosed by connective tissue capsules containing fibroblasts and collagenous fibres separated them from the surrounding ovarian structures. Both interstitial glands and their surrounding CT were penetrated by several blood vessels. There was a strong immunoreactive signal for S-100 in the nuclei of interstitial cells, and no signals were detected either in cells of the interstitial glands or their connective tissue with PR. We could conclude that the interstitial gland is distinct in ovary of camel and further studies are needed to elucidate its rule in steroid synthesis.

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Section: Discussionmentioning

confidence: 99%

Histological and immunohistochemical investigations of ovarian interstitial glands during non‐breeding season in camels (Camelus dromedarius)

Mohamed

Amin

Hussein

2018

Reprod Domestic Animals

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“…Disruption of this mechanism allows opportunistic pathogens to colonize the endometrium and cause an endometritis particularly with wrong mating as suggested by Ali (2010) in a nulliparous female camel. The endometrial gland dysgenesis was also reported to be correlated to the ovarian follicular dynamic that is highly decreased during the nonbreeding season (Ali et al, 2017;Gherissi et al, 2018b). The bibliographic data lack information on the incidence of endometrial adenogenesis and regeneration anomalies on the reproduction flow of camels.…”

Section: Discussionmentioning

confidence: 99%

“…As reported above, the pathways that orchestrate Müllerian duct formation and differentiation are obviously complex. The endometrial gland dysgenesis was reported to occur during the nonbreeding season of the camel species (Ali et al, 2017). Such reduced endometrial gland numbers were associated to pregnancy rate decease in adult heifers and ovine Uterine Gland Knockout (UGKO) (Floyd et al, 2001;Gray et al, 2000) and result in fibrotic lesions in mare endometrium (Gerstenberg et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Genital abnormalities associated to lack of uterine adenogenesis or endometrial gland dysgenesis of female dromedary camels (<i>Camelus dromedarius</i>)

et al. 2020

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Background: The developmental disruption of the müllerian duct and the endometrial dynamic can generate genital lesions that could contribute to infertility.Aim: This paper discusses two cases of genital conditions associated to endometrial gland pathologies in nulliparous female camels.Methods: Macroscopic examinations and histopathological description were performed on congenital and acquired genital abnormalities with endometrial gland anomalies.Results: The first case is endometrial gland agenesis associated to unilateral uterine aplasia, and the second case is endometrial gland dysgenesis associated to metritis. The prevalence of each case is estimated to be 0.6%. The most specific microscopic features associated to the endometrial gland agenesis were the presence of endometrial stromal proliferation and homogenous hyalinization of the myometrium. The acute metritis was associated to endometrial-activated stroma with focal infiltration with inflammatory cells on the endometrium and myometrium and the spontaneous endometrial gland dysgenesis.Conclusion: This study reveals the importance of congenital abnormalities during the routine reproductive examination of peripubertal animals, as well as the association of histopathological complementary examination for the research functional and inflammatory anomalies of the uterus. Genetic screening of breeders would be very important in the search for genetic risk factors associated with these congenital pathologies, which can be disseminated by reproductive biotechnologies. Keywords: Adenogenesis, Camel, Metritis, Uterine aplasia, Uterine glands.

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“…Moreover, routine histological processing including the heating of samples in liquid paraffin inactivates the virus in processed morbid samples (Ali et al, 2017;Henwood, 2018). Additionally, the virus can be inactivated by heat treatment at 56 C for 90 min, 67 C for 60 min, or 75 C for 30 min (Duan et al, 2003).…”

Section: Inactivation Of the Virusmentioning

confidence: 99%

Disinfection of corona virus in histopathology laboratories

et al. 2020

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Severe acute respiratory syndrome (SARS CoV-2/COVID-19) is a highly contagious and deadly disease caused by a virus belonging to the coronaviridae family.Researchers working in histopathology laboratories, dealing with morbid samples, are particularly vulnerable to infection unless they have very strong immunity. Hence, a proper precautionary protocol is required for the safety of the laboratory staff. The current review highlights the biological and physical agents that can be used to inactivate the virus and disinfect the surrounding environment in the laboratory.

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Effects of natural environment on reproductive histo-morphometric dynamics of female dromedary camel

Cited by 11 publications

References 47 publications

Histological and immunohistochemical investigations of ovarian interstitial glands during non‐breeding season in camels (Camelus dromedarius)

Histological and immunohistochemical investigations of ovarian interstitial glands during non‐breeding season in camels (Camelus dromedarius)

Genital abnormalities associated to lack of uterine adenogenesis or endometrial gland dysgenesis of female dromedary camels (<i>Camelus dromedarius</i>)

Disinfection of corona virus in histopathology laboratories

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