Effects of Native Entomopathogenic Fungal Strains and Neem Extract on Spodoptera frugiperda on Maize

Hernandez-Trejo, Antonia; Drouaillet, Benigno Estrada; López-Santillán, José Alberto; Rios-Velasco, Claudio; Rodríguez‐Herrera, Raúl; Osorio-Hernández, Eduardo

doi:10.3958/059.044.0113

Cited by 11 publications

(11 citation statements)

References 13 publications

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“…We did not find any significant difference in the corn cobs weight among the treatments, which is in agreement with Hernandez-Trejo et al [44] findings, who found that application of M. robertsii on maize plants did not show any significant difference in grain yield per hectare among the treatments tested, whereas Russo et al [8] found an increase in maize corn yield after applying B. bassiana under field conditions.…”

Section: Discussionsupporting

confidence: 92%

“…In addition, the most relevant fact in the second field trial was that we did not observe any S. frugiperda larvae, neither in the negative control plants nor in B. bassiana-treated plants, during the period of the study. Hernandez-Trejo et al, reported that Metarhizium robertsii decreased S. frugiperda incidence from 41.3% to 2.8% in the first application and 17.4% to 8.3% in the second application on maize plants [44]. Our findings need more experiments in the field to understand the mechanisms related to these results.…”

Section: Discussionmentioning

confidence: 54%

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Effect of Zea Mays-Beauveria Bassiana Seed Treatment on Spodoptera Frugiperda

Kuzhuppillymyal-Prabhakarankutty¹,

Ferrara-Rivero²,

Tamez‐Guerra³

et al. 2021

Preprint

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Spodoptera frugiperda is a widely distributed insect pest that causes major economic losses in various crops, particularly maize. On the other hand, Beauveria bassiana is an entomopathogenic fungus that establishes symbiotic associations with many plants and contributes to tolerance against biotic and abiotic stresses. In the present work, under field conditions, 1x106 (first trial) and 1x10⁸ (second trial) of B. bassiana (GHA strain) blastospores were used for corn’s seed inoculation. In the first field trial, a higher number of larvae were present in the negative control plants in comparison with those in B. bassiana-treated plants. No larvae were found in negative control and B. bassiana-treated plants in the second field trial. In further laboratory experiments, the effects of the B. bassiana strains GHA, in addition to a native strain (PTG4) also delivered via seed treatment in maize seedlings, on S. frugiperda growth, development, and mortality were evaluated. 1x10⁶ B. bassiana blastospores were used to inoculate maize seeds, which were germinated and grown to seedlings under growth chamber conditions. Third-instar S. frugiperda larvae were allowed to feed on B. bassiana-treated and -untreated (negative control) seedlings until reaching 6th instar and transferred to artificial diet until reaching adult stage. Results showed that larvae feeding on B. bassiana strain PTG4 prolonged their larval stage. Furthermore, feeding with plants treated with B. bassiana strains yielded fewer S. frugiperda male moths and the female moths emerged with altered wings, compared with the untreated control. In conclusion, seed treatment with B. bassiana in maize reduced S. frugiperda infestation of maize plants in field trials. Besides S. frugiperda development was affected in laboratory trials.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 54%

Effect of Zea Mays-Beauveria Bassiana Seed Treatment on Spodoptera Frugiperda

Kuzhuppillymyal-Prabhakarankutty¹,

Ferrara-Rivero²,

Tamez‐Guerra³

et al. 2021

Preprint

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“…The indigenous isolates of EPF from different localities or hosts deliver control programs with available and specific controlling methods against indigenous pests because they are more adapted to a range of environmental conditions (Zayed, 2003). Different studies also showed that indigenous isolates of fungal entomopathogen are effective against various agricultural pests under local conditions (Clifton et al, 2019;Hernandez-Trejo et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic, molecular, and virulence characterization of entomopathogenic fungi, Beauveria bassiana (Balsam) Vuillemin, and Metarhizium anisopliae (Metschn.) Sorokin from soil samples of Ethiopia for the development of mycoinsecticide

Gebremariam

Chekol

Assefa

2021

Heliyon

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Fungal entomopathogens are the most effective biocontrol agents against insect pests in the natural ecosystem. This study was conducted for phenotypic, molecular, and virulence characterization of locally isolated entomopathogenic fungi from soil samples of six localities in Ethiopia. Entomopathogenic fungi were isolated from 120 soil samples with the galleria baiting method. A total of 65 (54.2%) entomopathogenic fungal isolates belongs to Beauveria spp and Metarhizium spp were identified based on cultural and morphological features. All isolates were pre-screened based on germination, vegetative growth rate, and spore production as fungal in-vitro virulence determinates. Isolate AAUKB-11 displayed the peak germination of 99.67% and isolate AAUMFB-77 achieved the highest radial growth rate of 3.43 mm day À1 with the highest sporulation 4.60 Â 10 8 spores/ml. The phylogenetic analysis of ITS-rDNA confirmed that 7 isolates were identified as B. bassiana and 5 isolates were categorized into M. anisopliae. Selected B. bassiana and M. anisopliae strains were evaluated for their pathogenicity efficiency against G. mellonella larvae and caused 86.67%-100% mortality. The mortality rates of G. mellonella larvae peaked at 100% with 4(33.33%) isolates from B. bassiana and 2(16.67%) isolates from M. anisopliae after 10 days of treatments. The high virulent isolate, B. bassiana AAUMB-29 displayed the least LT 50 value of 2.36 days followed by isolate B. bassiana AAUMFB-77 with LT 50 of 2.53 days. Future studies should be needed to focus on the evaluation of high virulent isolates against other potential insect pests to assess their vigorous role as favorable biological control agents.

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“…However, the entomopathogenic fungi do not harm the egg and larval parasitoids of S. frugiperda (Ngangambe and Mwatawala 2020) and the predatory arthropods (Prabawati et al 2019). The entomopathogenic fungi can be integrated with botanical insecticides (Hernandez-Trejo et al 2019) and the entomopathogenic virus, such as nucleopolyhedroviruses (Souza et al 2019). The entomopathogenic fungi from the South Sumatra soil need to be explored and tested for their pathogenicity against the S. frugiperda.…”

Section: Introductionmentioning

confidence: 99%

Exploring entomopathogenic fungi from South Sumatra (Indonesia) soil and their pathogenicity against a new invasive maize pest, Spodoptera frugiperda

Suwandi

2020

Biodiversitas

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Abstract. Herlinda S, Octariati N, Suwandi S, Hasbi. 2020. Exploring entomopathogenic fungi from South Sumatra (Indonesia) soil and their pathogenicity against a new invasive maize pest, Spodoptera frugiperda. Biodiversitas 21: 2955-2965. Fall armyworm (Spodoptera frugiperda) is a new invasive maize pest in Indonesia that can cause maize yield losses of 18 million tons/year. To overcome the pest, local-specific entomopathogenic fungi are needed. This study aimed to explore entomopathogenic fungi from soil in South Sumatra and to determine their pathogenicity against S. frugiperda larvae. The fungi exploration was carried out in the lowlands and highlands of South Sumatra and the pathogenicity of obtained isolates were tested against the third instar larvae. The entomopathogenic fungi found were Metarhizium spp. and were successfully isolated as many as 14 isolates. All of the isolates were pathogenic to S. frugiperda larvae (70.67−78.67% mortality), the most pathogenic caused 78.67% mortality and significantly suppressed the emergence of adults up to 81.2%. Unhealthy larvae had a dry, shrunken, shrinking, odorless body, and its integument was covered in mycelia and conidia like yellowish-white powdery mixed with dark green. The unhealthy pupae and adults were in the abnormal and malformation shape. The abnormal pupae were shorter in size, bent, the to-be wings got wrinkled, and darker color, while the unhealthy adults had folded wings and were unable to fly. The two most pathogenic isolates were found from the lowland (PirOI) and highland (CasPsPGA) soil of South Sumatra. In conclusion, both of these isolates had the potential to be developed into local-specific mycoinsecticides to control pest insects in the highlands and/or lowlands in Indonesia.

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Effects of Native Entomopathogenic Fungal Strains and Neem Extract on Spodoptera frugiperda on Maize

Cited by 11 publications

References 13 publications

Effect of Zea Mays-Beauveria Bassiana Seed Treatment on Spodoptera Frugiperda

Effect of Zea Mays-Beauveria Bassiana Seed Treatment on Spodoptera Frugiperda

Phenotypic, molecular, and virulence characterization of entomopathogenic fungi, Beauveria bassiana (Balsam) Vuillemin, and Metarhizium anisopliae (Metschn.) Sorokin from soil samples of Ethiopia for the development of mycoinsecticide

Exploring entomopathogenic fungi from South Sumatra (Indonesia) soil and their pathogenicity against a new invasive maize pest, Spodoptera frugiperda

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