Effects of Naringenin on Lipogenesis, Lipolysis and Glucose Uptake in Rat Adipocyte Primary Culture: A natural antidiabetic agent

Abstract

CitationS Lim, K Soh, U Kuppusamy. Effects of Naringenin on Lipogenesis, Lipolysis and Glucose Uptake in Rat Adipocyte Primary Culture: A natural antidiabetic agent. AbstractThe flavonoid naringenin is commonly found in citrus fruits and tomato. The main aim of this study was to assess the in vitro insulin-like effects of naringenin using rat adipocyte primary culture. The adipogenic, lipolytic and glucose uptake activities of naringenin in primary rat adipocytes were assessed using Oil Red O, glycerol, and 2-deoxy-D-[2,6-3 H]glucose uptake quantitative assays respectively. Naringenin exhibited significant increase of lipogenesis in the presence and absence of insulin in primary rat preadipocytes. At the concentration range of 0.01 -100 µM, naringenin inhibited 50 % of epinephrine induced lipolysis in rat adipocytes and enhanced insulin's antilipolytic activity. Naringenin (100 µM) stimulated 163 % glucose uptake in rat adipocytes (compared to untreated cells) and this was significantly higher than the insulin mediated glucose uptake at similar concentration. Thus, naringenin may play an important role as an adjuvant and/or alternative to insulin therapy for the management of diabetes mellitus.

Citrus polyphenols and risk of type 2 diabetes: Evidence from mechanistic studies

“…At a concentration of 75 μM, naringenin 9 exhibited maximum glucose uptake, which was similar to the activity exerted by 100 nM insulin (Zygmunt et al 2010). Several other studies have also reported the insulin-like effects of naringenin 9 in HepG2 cells (Allister et al 2005;Borradaile, de Dreu, and Huff 2003) and rat adipocytes (Lim, Soh, and Kuppusamy 2008). In addition to its insulin-like effects, naringenin 9 significantly enhanced insulin-stimulated glucose uptake in muscle cells at low insulin levels (<10 nM) (Zygmunt et al 2010).…”

“…Microarray studies revealed further up-regulation of several genes linked with energy metabolism (Horiba et al 2010). At a concentration of 100 µM, naringenin 9 treatment led to increased glucose uptake (163%) in primary rat adipocytes, superior to insulin (Lim, Soh, and Kuppusamy 2008). Furthermore, naringenin 9 significantly increased glucose uptake in a dose-dependent manner (0.01 − 100 µM naringenin 9) independent of insulin (Lim, Soh, and Kuppusamy 2008).…”

“…At a concentration of 100 µM, naringenin 9 treatment led to increased glucose uptake (163%) in primary rat adipocytes, superior to insulin (Lim, Soh, and Kuppusamy 2008). Furthermore, naringenin 9 significantly increased glucose uptake in a dose-dependent manner (0.01 − 100 µM naringenin 9) independent of insulin (Lim, Soh, and Kuppusamy 2008). Naringenin 9 at >10 µM reduced Toll-like receptor 2 (TLR2) mRNA and protein expression in 3T3-L1 adipocyte during differentiation (Yoshida et al 2013).…”

Review of factors affecting citrus polyphenol bioavailability and their importance in designing in vitro, animal, and intervention studies

“…For example, the majority of the evidence on the antidiabetic role of citrus flavanones stems from the aglycones hesperetin 1 and naringenin 2. Several studies report that hesperetin 1 and naringenin 2 favorably modulate hepatic glucose homeostasis, pancreatic insulin secretion, and insulin sensitivity in tissues in a way that could reduce the risk of type 2 diabetes (Bhattacharya et al, 2014;Lim et al, 2008;Murugesan et al, 2020;Rebello et al, 2019;Yoshida et al, 2013). Importantly, more studies should examine the bioactivities of the colon-derived phenolic catabolites that are absorbed and circulate in the body in amounts several-fold higher than that of the parent flavanone metabolites (Figure 10), which only reach low μmol/L levels (Table 1).…”