Effects of Naphthalene Acetic Acid (NAA) and Indole -3- Butyric Acid (IBA) on In Vitro Rooting of Sugarcane (Saccharum officinarum L.) Micro- Shoots

Tolera, Belay

doi:10.4172/2155-952x.1000215

Cited by 4 publications

(2 citation statements)

References 22 publications

(19 reference statements)

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“…In the present study, half and full-strength MS medium were able to induce roots, but half strength medium was superior to full strength medium, which was similar to the findings reported for Gardenia latifolia (Reddy, Saritha 2013) and Terminalia bellerica (Phulwaria et al 2012). It is known that IBA and NAA stimulates induction of adventitious roots (Copes, Mandel 2000) and they have been utilised for root induction in Rauwolfia serpentina (Pandey et al 2010), sugarcane (Tolera 2016;Rahman et al 2018), Tagetes erecta (Ullah et al 2013) and also tree species like Sterculia urens (Hussain et al 2008) and Balanites aegyptica (Mansor et al2003). In S. suaveolens, both of the auxins (IBA and NAA) were able to induce roots, but compared to NAA, IBA was effective in inducing the highest number of roots (9.3) at 2.5 µM.…”

Section: Discussionsupporting

confidence: 88%

Synergistic effect of cytokinins on in vitro propagation of Stereospermum suaveolens using nodal explants

2021

EEB

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The Stereospermum suaveolens DC. is one of the important medicinal tree species and also one of the important constituents of Dashmoolarisht. Owing to its importance an attempt was made to perform in vitro regeneration from nodal explants. Cultures were established by placing the nodal explants on Murashige and Skoog's (MS) and Woody Plant media fortified with different combinations of cytokinins. The in vitro shoots developed were excised into single in vitro nodes and then utilised for multiplication of shoots. Among the various combinations tested in both of the media, the MS medium fortified with benzylaminopurine (8 µM) + thidiazuron (0.2 µM) proved to be effective in inducing a maximum of 10.2 number of shoots by the end of the fifth subculture. In vitro rooting of shoots was best achieved in ½ MS liquid medium fortified with indolebutyric acid (2.5µM), with the highest number of roots (9.3) in 100% cultures. Well rooted in vitro plantlets were successfully hardened under greenhouse conditions, resulting in 100% survival of plants after four weeks of transfer to cocopeat/soil substrate.

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Section: Discussionsupporting

confidence: 88%

Synergistic effect of cytokinins on in vitro propagation of Stereospermum suaveolens using nodal explants

2021

EEB

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“…Clonal propagation can be successfully achieved through optimal use of phytohormones such as naphthalene acetic acid (NAA) which are extensively used for root induction. It was used in micropropagation protocols of Zingiber spectabile ( Faria and Illg, 1995 ), sugarcane ( Tolera, 2016 ), and gerberas ( Singh et al, 2016 ). Sarropoulou and Maloupa (2019) recorded maximum root numbers with 0.5 mg/l NAA while propagating Satureja thymbra .…”

Section: Introductionmentioning

confidence: 99%

Clonal Propagation of Valeriana jatamansi Retains the Essential Oil Profile of Mother Plants: An Approach Toward Generating Homogenous Grade of Essential Oil for Industrial Use

Gautam

Kumar

et al. 2021

Front. Plant Sci.

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Valeriana jatamansi Jones (Syn. V. wallichii DC.) is an aromatic, medicinal herb used as a tranquilizer and in treating sleep disorders. Rhizome is mainly used to extract essential oil (EO) and valepotriates. High quality and economic yield of rhizomes are available in the third year of growth. Therefore, the cultivation of V. jatamansi is not picking up, and over-exploitation of this plant from wild habitats to meet the increasing demand of the pharmaceutical industry is the cause of threat to the genetic diversity of the species. Further, collections from the wild are heterogeneous, resulting in variable produce. The development of clonal lines can ensure uniform quality and yield of rhizome biomass. An effective clonal propagation method was standardized using different hormonal concentrations of naphthalene acetic acid (NAA) on apical shoot cuttings from the selected clone CSIR-IHBT-VJ-05 for different time durations and raised over various planting media. NAA treatment of 50 ppm concentration for 30 min was found optimum for root induction in apical shoots of V. jatamansi. Variations for EO composition within the clone were non-significant, while samples of the control population were variable. The best quality EO (patchouli alcohol ∼62%) was available during the third year of plant growth. A propagation technique for large-scale quality plant material (QPM) production has been standardized to reduce the stress over natural resources and promote V. jatamansi for use in the aromatic and pharmaceutical industry.

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In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.

Akın

Çetin

Bingöl³

2018

Celal Bayar Üniversitesi Fen Bilimleri Dergisi

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The aim of this experiment is to develop a protocol to establish the in vitro propagation potential of Lythrum salicaria L (purple loosestrife). Seeds of L. salicaria were germinated and grown in Murashige Skoog (MS) medium for 30 days. At the end of 30 days, shoot tips of L. salicaria were cultured on MS medium supplemented with different combinations and concentrations of 6-benzylaminopurine (BAP; 1, 2 and 3 mg/L) and 1-naphthaleneacetic acid (NAA; 0, 0.1 and 0.5 mg/L). Regenerated shoots were transferred to MS supplemented with varying concentrations of different auxins: NAA (0.5 and 1 mg/L) and indole-3-butyric acid (IBA; 0.5 and 1.0 mg/L) for rooting. Among the cytokinins investigated, BAP indicated the best response to initiate shoot elongation and multiple shoot formation alone. The highest number of shoot per explant (37±3.38) was obtained in MS medium with 1 mg/L BAP. Although, the highest shoot length (3.1 cm ± 0.48) was found on medium with 1 mg/L BAP+0.5 mg/L NAA, there were no statistically differences among all types of medium. Percentage of rooting, shoot length and root length were altered significantly with different concentrations of NAA and IBA. Thus, shoot length (9.5 cm±0.46), root length (4.1 cm±0.13) and the percentage of rooting (100%) increased significantly with a concentration of 1 mg/L NAA. It was concluded that MS medium with 1 mg/L NAA has been superior for root development compared to other hormone concentrations.

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Effects of Naphthalene Acetic Acid (NAA) and Indole -3- Butyric Acid (IBA) on In Vitro Rooting of Sugarcane (Saccharum officinarum L.) Micro- Shoots

Cited by 4 publications

References 22 publications

Synergistic effect of cytokinins on in vitro propagation of Stereospermum suaveolens using nodal explants

Synergistic effect of cytokinins on in vitro propagation of Stereospermum suaveolens using nodal explants

Clonal Propagation of Valeriana jatamansi Retains the Essential Oil Profile of Mother Plants: An Approach Toward Generating Homogenous Grade of Essential Oil for Industrial Use

In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.

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