Effects of nanoscale thickness and elastic nonlinearity on measured mechanical properties of polymeric films

Oommen, Binu; Vliet, Krystyn J. Van

doi:10.1016/j.tsf.2006.01.069

Cited by 45 publications

(33 citation statements)

References 23 publications

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“…One way to understand the substrate influence on E values of lipid films is to prepare and examine films with different thicknesses. When working with thick samples, indentations are limited to depths much smaller than the total thickness of the sample (10-20%) [31,32], in order to eliminate the influence of the substrate. This important issue has been addressed especially by the researchers in the group of Tsukruk [32], who have exploited a semi-empirical formula of Shull et al [33] to model the deformations of thin polymeric films and of polymer nanocomposite layers.…”

Section: Introductionmentioning

confidence: 99%

Nanoscale mechanics of solid-supported multilayered lipid films by force measurement

et al. 2008

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Section: Introductionmentioning

confidence: 99%

Nanoscale mechanics of solid-supported multilayered lipid films by force measurement

et al. 2008

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“…The indentation speed was set to ≈40 nm s −1 and the indentation depth was set to less than 200 nm to avoid destroying the cell. In addition, it is generally accepted that 10% of indentation depth to the thickness of the target material can give consistent result regardless of substrate effect . Therefore, indentation depth was controlled to be less than 200 nm to eliminate influence of the substrate or cell height.…”

Section: Methodsmentioning

confidence: 99%

Microsphere‐Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1‐MMP

Kim

Yau

et al. 2018

Small

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Biophysical properties are intimately connected to metastatic functions and aggressiveness in cancers. Especially, cellular stiffness is regarded as a biomarker for the understanding of metastatic potential and drug sensitivity. Here, protease‐mediated changes of cortical stiffness are identified due to the deformation of cytoskeleton alignment at a cortex. For the past few decades, membrane type 1‐matrix metalloproteinase (MT1‐MMP) has been well known as a kernel protease enriched in podosomes during metastasis for extracellular matrix degradation. However, the biophysical significance of MT1‐MMP expressing cancer cells is still unknown. Therefore, the nanomechanics of cancer cells is analyzed by a nanoindentation using a microsphere‐attached cantilever of atomic force microscopy (AFM). In conclusion, the results suggest that MT1‐MMP has contributed as a key regulator in cytoskeletal deformation related with cancer metastasis. Particularly, the AFM‐based nanoindentation system for the monitoring of cortical nanomechanics will be crucial to understand molecular networks in cancers.

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“…21,39,44,[68][69][70][71][72] Dimitriadis et al 42 developed an analytic approximate correction for spherical probes in the limit of small indentation, to take into account the aforementioned effect due to the finite thickness of the sample (similar corrections have been reported for conical indenters; 72,73 for a study not limited to small indentation, see Ref. 71).…”

Section: B Finite-thickness Effectmentioning

confidence: 96%

Nanomechanical and topographical imaging of living cells by atomic force microscopy with colloidal probes

Puricelli

Galluzzi

Schulte

et al. 2015

Review of Scientific Instruments

161

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Atomic Force Microscopy (AFM) has a great potential as a tool to characterize mechanical and morphological properties of living cells; these properties have been shown to correlate with cells' fate and patho-physiological state in view of the development of novel early-diagnostic strategies. Although several reports have described experimental and technical approaches for the characterization of cellular elasticity by means of AFM, a robust and commonly accepted methodology is still lacking.Here we show that micrometric spherical probes (also known as colloidal probes) are well suited for performing a combined topographic and mechanical analysis of living cells, with spatial resolution suitable for a complete and accurate mapping of cell morphological and elastic properties, and superior reliability and accuracy in the mechanical measurements with respect to conventional and widely used sharp AFM tips. We address a number of issues concerning the nanomechanical analysis, including the applicability of contact mechanical models and the impact of a constrained contact geometry on the measured Young's modulus (the finite-thickness effect). We have tested our protocol by imaging living PC12 and MDA-MB-231 cells, in order to demonstrate the importance of the correction of the finite-thickness effect and the change in Young's modulus induced by the action of a cytoskeleton-targeting drug.

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Effects of nanoscale thickness and elastic nonlinearity on measured mechanical properties of polymeric films

Cited by 45 publications

References 23 publications

Nanoscale mechanics of solid-supported multilayered lipid films by force measurement

Nanoscale mechanics of solid-supported multilayered lipid films by force measurement

Microsphere‐Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1‐MMP

Nanomechanical and topographical imaging of living cells by atomic force microscopy with colloidal probes

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