Effects of N-carbamylglutamate and arginine on steroidogenesis and proliferation of pig granulosa cells in vitro

Feng, Tao; DeVore, A.A.; Perego, Maria Chiara; Morrell, B.C.; Spicer, L. J.

doi:10.1016/j.anireprosci.2019.106138

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Dietary supplementation with NCG has also been reported to increase blood estradiol concentrations in yaks ( 25 ) and chickens ( 57 ). Supplementation of NCG also promoted cell proliferation and inhibited apoptosis of pig placental trophoblast cells ( 58 ), and stimulated cell proliferation of bovine granulosa cells ( 59 ), both of which can synthesize estrogen. However, the precise molecular mechanism underlying this phenomenon has not been unveiled.…”

Section: Discussionmentioning

confidence: 99%

Integration of transcriptomic and metabolomic analysis of the mechanism of dietary N-carbamoylglutamate in promoting follicle development in yaks

Zhou

Yue²,

et al. 2022

Front. Vet. Sci.

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Yak is the main livestock in the highlands of China. The low reproductive rate of yaks is a serious constraint on their production and utility. N-carbamylglutamate (NCG) can increase arginine synthesis in mammals and has been shown to improve reproductive performance. Twelve multiparous and simutaneous anoestrous female yaks were randomly divided into two groups, one of which was fed the basal diet (Control, n = 6), and the other was fed the basal diet supplemented with NCG at 6 g/day/yak (NCG, n = 6). All yaks were slaughtered on the 32nd day (the time predicted for the selection of the last wave of dominant follicles), and their ovarian tissues were collected and follicles were classified. NCG supplementation increased the number of large ovarian follicles (diameter > 10 mm), as well as caused significant changes in the transcriptional and metabolic levels in yak ovaries which due to the differential expression of 889 genes and 94 metabolites. Integrated analysis of the transcriptomics and metabolomics data revealed that the differentially expressed genes and differential metabolites were primarily involved in the process of energy metabolism, amino acid metabolic pathways, carbohydrate metabolic pathways, and lipid metabolic pathways. The highlighted changes were associated with amino acid synthesis and metabolism, ovarian steroid hormone synthesis, the pentose phosphate pathway, and the tricarboxylic acid cycle, suggesting that NCG supplementation may promote estrogen synthesis and help regulate follicular development by altering the pathways associated with glucose catabolism. The results present important clues for understanding the mechanisms by which NCG supplementation promotes follicular development in yaks. The findings of this study provide a basis for the development and application of NCG in optimizing animal reproduction, including yak reproductive performance, which may help optimize livestock management and uplift the pastoral economy.

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Section: Discussionmentioning

confidence: 99%

Integration of transcriptomic and metabolomic analysis of the mechanism of dietary N-carbamoylglutamate in promoting follicle development in yaks

Zhou

Yue²,

et al. 2022

Front. Vet. Sci.

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“…The three pathways “cysteine and methionine metabolism”, “arginine and proline metabolism” and “glutathione metabolism” may be involved in the egg production process of hens. Feng et al found that arginine improved the reproductive performance of hind sows by adding arginine (ARG) to the diet [ 34 ]. There is evidence that ovarian glutathione is an important defense against oxidative damage, that glutathione in oocytes is essential in early embryonic development, and that glutathione biosynthesis is heavily dependent on the metabolism of cysteine and methionine [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics and Metabolomics Analysis of the Ovaries of High and Low Egg Production Chickens

Huang

Zhang

Cao

et al. 2022

Animals

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Egg production is a pivotal indicator for evaluating the fertility of poultry, and the ovary is an essential organ for egg production and plays an indispensable role in poultry production and reproduction. In order to investigate different aspects of egg production mechanisms in different poultry, in this study we performed a metabolomic analysis of the transcriptomic combination of the ovaries of two chicken breeds, the high-production Ninghai indigenous chickens and the low-production Wuliangshan black-boned chickens, to analyze the biosynthesis and potential key genes and metabolic pathways in the ovaries during egg production. We predicted four genes in the transcriptomic that are associated with egg production, namely P2RX1, INHBB, VIPR2, and FABP3, and identified three important pathways during egg production, “Calcium signaling pathway”, “Neuroactive ligand–receptor interaction” and “Cytokine–cytokine receptor interaction”, respectively. In the metabolomic 149 significantly differential metabolites were identified, 99 in the negative model and 50 in the positive model, of which 17α-hydroxyprogesterone, iloprost, spermidine, and adenosine are important metabolites involved in reproduction. By integrating transcriptomics and metabolomics, the correlation between specific differential genes and differential metabolites identified important gene-metabolite pairs “VIPR2-Spermidine” and “P2RX1-Spermidine” in egg production. In conclusion, these data provide a better understanding of the molecular differences between the ovaries of low- and high-production hens and provide a theoretical basis for further studies on the mechanics of poultry egg production.

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Effects of N-carbamylglutamate on steroidogenesis and relative abundances of mRNA transcripts in pig placental trophoblasts

Wei

Yang

Yinxia

et al. 2020

Animal Reproduction Science

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Effects of N-carbamylglutamate and arginine on steroidogenesis and proliferation of pig granulosa cells in vitro

Cited by 5 publications

References 39 publications

Integration of transcriptomic and metabolomic analysis of the mechanism of dietary N-carbamoylglutamate in promoting follicle development in yaks

Integration of transcriptomic and metabolomic analysis of the mechanism of dietary N-carbamoylglutamate in promoting follicle development in yaks

Transcriptomics and Metabolomics Analysis of the Ovaries of High and Low Egg Production Chickens

Effects of N-carbamylglutamate on steroidogenesis and relative abundances of mRNA transcripts in pig placental trophoblasts

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