Effects of N-Butanol and Aqueous Fractions of Khaya senegalensis, Guiera senegalensis and Tamarindus Indica Leaves Extracts on Eimeria tenella Oocyst Sporulation in Vitro

Abstract

The in vitro anti coccidial activities of n-butanol and aqueous fractions of Khaya senegalensis, Gueira senegalensis and Tamarindus indica leaves extracts on Eimeria tenella parasite was studied by observing the effects of the plant extracts on the sporulation of the Eimeria tenella oocysts. Drug resistance and consumer demanding decrease in the use of drugs in animals have generated interest in alternative strategies to control the avian disease one of which is herbal intervention studies. Fresh faecal samples were collected from infected birds and their oocysts load determined. Dilutions of the extracts (100mg, 250mg, 400mg, 550mg, 700mg and 850mg per ml) in distilled water were prepared and placed in separate well labelled petri dishes. 100 oocysts were added to each petri dish and the set up was left at ambient temperature on the laboratory table and monitored twice daily (9.00am and 3.00pm) to observe the sporulation of the oocysts over a 72 hours period. Laboratory sporulation medium (2.5% Potassium dichromate) and Amprolium were used for comparison. The phytochemical result implied that the active ingredients were present mainly in the n-butanol and crude extract fractions with the nbutanol fraction of the Khaya senegalensis extract containing phenols and flavonoids which have antioxidant effects. The in vitro study showed that the n-butanol fraction of K senegalensis (100mg/ml) and the aqueous fraction of Tamarindus indica (100mg/ml) extracts had similar effects to those obtained using Amprolium which was considered as the standard by inhibiting the sporulation of Eimeria tenella oocysts. It is therefore recommended that more work needs to be done to determine the anti coccidial activities of these extracts in vivo.
 Key words: Eimeria tenella, sporulation, in vitro, extracts

The Anticoccidial Effect of Alcoholic Vitis vinifera Leaf Extracts on Eimeria papillate Oocysts Isolated in Mice In Vitro and In Vivo

“…This might be a result of the lack of bioactive substances in them or of low levels of such compounds. In contradiction to what was stated in [ 53 ], there was in fact anticoccidial action seen in vitro of K. senegalensis , the current investigation found that the plant extracts have anticoccidial activity against E. papillata oocysts. This may be an outcome of the permeation of the cell walls of the oocysts by the fundamental plant components causing damage to the cytoplasm, which was evidenced by the manifestation of abnormal sporocysts in oocysts exposed to 200 and 150 dilutions in most of the plant extracts [ 42 ].…”