Effects of N and P availability on carbon allocation in the toxic dinoflagellate Ostreopsis cf. ovata

Pezzolesi, Laura; Vanucci, Silvana; Dell’Aversano, Carmela; Iacovo, Emma Dello; Tartaglione, Luciana; Pistocchi, Rossella

doi:10.1016/j.hal.2016.02.011

Cited by 15 publications

(11 citation statements)

References 85 publications

(141 reference statements)

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“…cf. ovata and nutrients temporal trend observed in this study are consistent with those previously described for the same algal species under comparable culture conditions (Pezzolesi et al., , ; Vanucci, Pezzolesi, et al., ). Bacterial community growth showed a biphasic pattern, characterized by two exponential growth steps having different growth rates that appear mainly triggered by different quality and amount of available substrate.…”

Section: Discussionsupporting

confidence: 93%

“…cf. ovata toxins (Pinna et al., ), are also known to be increasingly released from the exponential to the stationary phase (Pezzolesi et al., , ; Vanucci, Pezzolesi, et al., ; Vanucci, Guerrini, et al., ). Successively, Rhodobacteraceae genera such as Labrenzia and Dinoroseobacter may have also responded to a renewed availability of low molecular weight compounds by Bacteroidetes algal‐derived matter remineralization (Buchan et al., ; Fernández‐Gómez et al., ; Teeling et al., ) at the algal stationary phase.…”

Section: Discussionmentioning

confidence: 99%

“…cf. ovata produces palytoxin‐like compounds, namely, isobaric palytoxin and a wide range of ovatoxins (OVTX‐a to ‐k; Ciminiello et al., ; Brissard et al., ; Tartaglione et al., ) under both field (Accoroni et al., ; Carnicer, Guallar, Andree, Diogène, & Fernández‐Tejedor, ; Ciminiello et al., , ) and laboratory conditions (Pezzolesi et al., , ; Pistocchi et al., ; Vanucci, Pezzolesi, et al., ; Vanucci, Guerrini, et al., ). The epiphytic/benthic dinoflagellate grows onto a wide range of substrata, forming thick mucilaginous mats (Giussani et al., ; Honsell et al., ; Totti, Accoroni, Cerino, Cucchiari, & Romagnoli, ).…”

Section: Introductionmentioning

confidence: 99%

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Microbial dynamics during harmful dinoflagellate Ostreopsis cf. ovata growth: Bacterial succession and viral abundance pattern

2018

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Algal–bacterial interactions play a major role in shaping diversity of algal associated bacterial communities. Temporal variation in bacterial phylogenetic composition reflects changes of these complex interactions which occur during the algal growth cycle as well as throughout the lifetime of algal blooms. Viruses are also known to cause shifts in bacterial community diversity which could affect algal bloom phases. This study investigated on changes of bacterial and viral abundances, bacterial physiological status, and on bacterial successional pattern associated with the harmful benthic dinoflagellate Ostreopsis cf. ovata in batch cultures over the algal growth cycle. Bacterial community phylogenetic structure was assessed by 16S rRNA gene ION torrent sequencing. A comparison between bacterial community retrieved in cultures and that one co‐occurring in situ during the development of the O. cf. ovata bloom from where the algal strain was isolated was also reported. Bacterial community growth was characterized by a biphasic pattern with the highest contributions (~60%) of highly active bacteria found at the two bacterial exponential growth steps. An alphaproteobacterial consortium composed by the Rhodobacteraceae Dinoroseobacter (22.2%–35.4%) and Roseovarius (5.7%–18.3%), together with Oceanicaulis (14.2‐40.3%), was strongly associated with O. cf. ovata over the algal growth. The Rhodobacteraceae members encompassed phylotypes with an assessed mutualistic‐pathogenic bimodal behavior. Fabibacter (0.7%–25.2%), Labrenzia (5.6%–24.3%), and Dietzia (0.04%–1.7%) were relevant at the stationary phase. Overall, the successional pattern and the metabolic and functional traits of the bacterial community retrieved in culture mirror those ones underpinning O. cf. ovata bloom dynamics in field. Viral abundances increased synoptically with bacterial abundances during the first bacterial exponential growth step while being stationary during the second step. Microbial trends also suggest that viruses induced some shifts in bacterial community composition.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microbial dynamics during harmful dinoflagellate Ostreopsis cf. ovata growth: Bacterial succession and viral abundance pattern

2018

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“…Toxin profiles did not vary significantly among cells collected during the bloom (i.e., on artificial substrates, see Supplementary data S2), among cells collected on P. pavonica according to depth or among cells attached to the three macroalgae. Similar observations have previously been made in situ (Accoroni et al, 2011;Pfannkuchen et al, 2012;Brissard et al, 2014) and in controlled experiments (Pezzolesi et al, 2012;Vanucci et al, 2012;Pezzolesi et al, 2014;Carnicer et al, 2016;Pezzolesi et al, 2016;Mendes et al, 2017).…”

Section: Toxin Profilessupporting

confidence: 88%

Toxin content of Ostreopsis cf. ovata depends on bloom phases, depth and macroalgal substrate in the NW Mediterranean Sea

et al. 2020

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Over the last fifteen years, blooms of the genus Ostreopsis have been reported more frequently and at higher abundances in the Mediterranean area. Ostreopsis cf. ovata is known to produce ovatoxins (OVTXs), structural analogues of palytoxin, which is one of the most potent non-polymeric toxins. However, the production of OVTXs is poorly characterized in situ. The present study focuses on toxin content and profile according to the bloom phase during summer 2017 in Villefranche-sur-Mer, France (NW Mediterranean Sea), depth (from 0.5 to 5 m) and three different macroalgal substrates of this epiphytic dinoflagellate (Padina pavonica, Dictyota spp. and Halopteris scoparia). Ovatoxin quantification of all samples was performed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The bloom started at the end of June and declined in mid-July, showing the typical seasonal pattern of the NW Mediterranean Sea area. The peak was observed on the 10 July with 1.8 × 106 cells/g FW and 1.7 × 104 cells/L for benthic and planktonic cells, respectively. Total toxin content of cells, collected using artificial substrates, increased during the exponential and stationary growth phases. After reaching a maximum concentration of 9.2 pg/cell on 18 July, toxin concentration decreased and remained stable from 25 July until the end of monitoring. A decreasing trend of the abundance and of the associated total toxin content was noted with depth. Finally, the decreasing order of maximal epiphytic concentration of O. cf. ovata was: Dictyota spp. (8.3 × 105 cells/g FW), H. scoparia (3.1 × 105 cells/g FW) and P. pavonica (1.6 × 105 cells/g FW). Interestingly, the highest OVTX quota was obtained in cells present on Halopteris scoparia, then on Dictyota spp. and Padina pavonica. This suggests that the nature of the macroalgal substrate influences both growth and toxin production of O. cf. ovata and further work will be required to understand the underlying mechanisms (e.g., competition for nutrition, pH or allelopathic interaction). However, the toxin profiles (i.e., the proportion of each ovatoxin analogue) were not affected by any of the studied parameters (bloom phase, depth, macroalgae or artificial substrates). Highlights► Ostreopsis cf. ovata bloomed from late June to August 2017 at Villefranche-sur-Mer, France, Mediterranean Sea. ► Ovatoxins contents were higher during the exponential and stationary growth phases. ► Highest abundance was on Dictyota spp. but highest toxin quota on Halopteris scoparia. ► Ovatoxin profile was not affected by the bloom phase, the depth or the macroalgae.

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“…Here, the reported results concerning the effect of Cu and BuT on toxin content (pg.cell -1 ) of the studied microalgae are related to intracellular fraction of toxins. It will be interesting in a future work to consider the toxins released extracellularly (Pezzolesi et al, 2016) to bring information on the entire amount of toxins produced by the algae especially since the pollutants tested and particularly the butyltin causes cell lysis. Complementary experiments have to consider the estimation of the specific toxin production (µtox) of the two studied dinoflagellates grown with the pollutants (Basti et al 2015).…”

Section: Toxinsmentioning

confidence: 99%

Effects of copper and butyltin compounds on the growth, photosynthetic activity and toxin production of two HAB dinoflagellates: The planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata

et al. 2018

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Controlled laboratory experiments were conducted to test the effects of copper (Cu) and butyltins (BuT) on the growth, photosynthetic activity and toxin content of two HABs (Harmful Algal Blooms) dinoflagellates, the planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata. Microalgae were exposed to increasing concentrations of Cu (10 to 31 nM) or BuT (0.084 to 84 nM) for seven days. When considering the growth, EC values were 0.16 (±0.09) nM and 0.03 (±0.02) nM of Cu for A. catenella and O. cf. ovata, respectively. Regarding BuT, EC was 14.2 (±6) nM for O. cf. ovata, while A. catenella growth inhibition appeared at BuT concentrations ≥27 nM. Photosynthetic activity of the studied dinoflagellates decreased with increasing Cu and BuT concentrations. For O. cf. ovata, the response of this physiological parameter to contamination was less sensitive than the biomass. Cu exposure induced the formation of temporary cysts in both organisms that could resist adverse conditions. The ovatoxin-a and -b concentrations in O. cf. ovata cells increased significantly in the presence of Cu. Altogether, the results suggest a better tolerance of the planktonic A. catenella to Cu and BuT. This could result in a differentiated selection pressure exerted by these metals on phytoplankton species in highly polluted waters. The over-production of toxins in response to Cu stress could pose supplementary health and socio-economic threats in the contaminated marine ecosystems where HABs develop.

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Effects of N and P availability on carbon allocation in the toxic dinoflagellate Ostreopsis cf. ovata

Cited by 15 publications

References 85 publications

Microbial dynamics during harmful dinoflagellate Ostreopsis cf. ovata growth: Bacterial succession and viral abundance pattern

Microbial dynamics during harmful dinoflagellate Ostreopsis cf. ovata growth: Bacterial succession and viral abundance pattern

Toxin content of Ostreopsis cf. ovata depends on bloom phases, depth and macroalgal substrate in the NW Mediterranean Sea

Effects of copper and butyltin compounds on the growth, photosynthetic activity and toxin production of two HAB dinoflagellates: The planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata

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