“…However, in another study, individual supplementation of oleic acid (OA; 18:1), DHA and EPA to placental explant culture media was able to reduce the production of interleukin6 (IL6) while combination of these fatty acids was found to have no effect on the inflammatory cytokine levels . A supplementation study in sheep showed that LA inhibits both prostaglandin1 (PGE1) and PGE2 production while AA stimulates PGE1 and PGE2 production in the placenta . Thus, these studies suggest that the effect of LCPUFA supplementation may vary depending on the dose and combination of the LCPUFA, presence of antioxidants, developmental stage, and experimental system.…”
Section: Regional Development and Function Of The Placenta: Possible Role Of Lcpufasmentioning
The placenta is an essential organ formed during pregnancy that mainly transfers nutrients from the mother to the fetus. Nutrients taken up by the placenta are required for its own growth and development and to optimize fetal growth. Hence, placental function is an important determinant of pregnancy outcome. Among various nutrients, fatty acids, especially long-chain polyunsaturated fatty acids (LCPUFAs), including omega 3 and omega 6 fatty acids, are essential for placental development from the time of implantation. Studies have associated these LCPUFAs with placental development through their roles in regulating oxidative stress, angiogenesis, and inflammation, which may in turn influence their transfer to the fetus. The placenta has a heterogeneous morphology with variable regional vasculature, oxidative stress, and LCPUFA levels in healthy pregnancies depending upon the location within the placenta. However, these regional structural and functional parameters are found to be disturbed in pathological conditions, such as preeclampsia (PE), thereby affecting pregnancy outcome. Hence, the alterations in LCPUFA metabolism and transport in different regions of the PE placenta as compared with normal placenta could potentially be contributing to the pathological features of PE. The regional variations in development and function of the placenta and its possible association with placental LCPUFA metabolism and transport in normal and PE pregnancies are discussed in this review. WIREs Dev Biol 2016, 5:582-597. doi: 10.1002/wdev.238 For further resources related to this article, please visit the WIREs website.
“…However, in another study, individual supplementation of oleic acid (OA; 18:1), DHA and EPA to placental explant culture media was able to reduce the production of interleukin6 (IL6) while combination of these fatty acids was found to have no effect on the inflammatory cytokine levels . A supplementation study in sheep showed that LA inhibits both prostaglandin1 (PGE1) and PGE2 production while AA stimulates PGE1 and PGE2 production in the placenta . Thus, these studies suggest that the effect of LCPUFA supplementation may vary depending on the dose and combination of the LCPUFA, presence of antioxidants, developmental stage, and experimental system.…”
Section: Regional Development and Function Of The Placenta: Possible Role Of Lcpufasmentioning
The placenta is an essential organ formed during pregnancy that mainly transfers nutrients from the mother to the fetus. Nutrients taken up by the placenta are required for its own growth and development and to optimize fetal growth. Hence, placental function is an important determinant of pregnancy outcome. Among various nutrients, fatty acids, especially long-chain polyunsaturated fatty acids (LCPUFAs), including omega 3 and omega 6 fatty acids, are essential for placental development from the time of implantation. Studies have associated these LCPUFAs with placental development through their roles in regulating oxidative stress, angiogenesis, and inflammation, which may in turn influence their transfer to the fetus. The placenta has a heterogeneous morphology with variable regional vasculature, oxidative stress, and LCPUFA levels in healthy pregnancies depending upon the location within the placenta. However, these regional structural and functional parameters are found to be disturbed in pathological conditions, such as preeclampsia (PE), thereby affecting pregnancy outcome. Hence, the alterations in LCPUFA metabolism and transport in different regions of the PE placenta as compared with normal placenta could potentially be contributing to the pathological features of PE. The regional variations in development and function of the placenta and its possible association with placental LCPUFA metabolism and transport in normal and PE pregnancies are discussed in this review. WIREs Dev Biol 2016, 5:582-597. doi: 10.1002/wdev.238 For further resources related to this article, please visit the WIREs website.
“…Only PGE2 production was measured in FC cells as we have shown previously that production of PGF2α is negligible/undetectable [30]. FC cells were also not challenged with OT as they are unresponsive (data not shown).…”
Section: Effect Of Oa Supplementation On Pge2 Production By Fc Cellsmentioning
“…Linoleic acid, a component of the supplemented NEFA mixture, may have reduced the activity of COX2-catalyzed PG biosynthesis. Limited PG biosynthesis in the presence of linoleate was demonstrated previously in bovine pulmonary artery endothelial cells and placental structures (Kaduce et al, 1982;Cheng et al, 2011). The presence of albumin in the NEFA mixture also could explain reductions in PG biosynthesis because of its potent activity as a ligand binder that could have reduced the availability of PGH 2 for prostaglandin synthases (Roche et al, 2008).…”
Intense lipid mobilization during the transition period in dairy cows is associated with increased disease susceptibility. The potential impact of altered plasma nonesterified fatty acids (NEFA) concentrations and composition on host inflammatory responses that may contribute to disease incidence and severity are not known. The objective of this study was to evaluate if increased NEFA concentrations could modify vascular inflammatory responses in vitro by changing the expression of important inflammatory mediators that are important in the pathogenesis of infectious diseases of transition cows such as mastitis and metritis. Bovine aortic endothelial cells (BAEC) were cultured with different concentrations of a NEFA mixture that reflected the plasma NEFA composition during different stages of lactation. The expression of cytokines, adhesion molecules, and eicosanoids were measured to assess changes in BAEC inflammatory phenotype. Addition of NEFA mixtures altered the fatty acid profile of BAEC by increasing the concentration of stearic acid (C18:0) and decreasing the content of arachidonic acid (C20:4n6c) and other long-chain polyunsaturated fatty acids in the phospholipid fraction. A significant increase also occurred in mRNA expression of cytokine and adhesion molecules that are associated with increased inflammatory responses during the transition period. Expression of cyclooxygenase 2, an important enzyme associated with eicosanoid biosynthesis, was increased in a NEFA concentration-dependent manner. The production of linoleic acid-derived eicosanoids 9- and 13-hydroxyoctadecadienoic acids also was increased significantly after treatment with NEFA mixtures. This research described for the first time specific changes in vascular inflammatory response during in vitro exposure to NEFA mixtures that mimic the composition and concentration found in cows during the transition period. These findings could explain, in part, alterations in inflammatory responses observed during intense lipid mobilization stages such as in the transition period of dairy cows. Future studies should analyze specific mechanisms by which high NEFA concentrations induce a vascular proinflammatory phenotype including the effect of 9 and 13-hydroxyoctadecadienoic acids and other lipid mediators.
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