Effects of Myo-Inositol on NaCl Stress in Tamarix ramosissima: Insights from Transcriptomics and Metabolomics

Abstract

NaCl stress adversely affects plant growth. Tamarix ramosissima Ledeb (T. ramosissima), a halophyte, thrives in saline-alkali areas. Myo-inositol, a lipid-soluble compound, is crucial for stress response, but its role in mitigating NaCl damage remains underexplored. We analyzed transcriptome sequencing and metabolites in T. ramosissima roots under NaCl stress at various intervals (0 h, 48 h, and 168 h). We identified ten Myo-inositol oxygenase-related genes. Nine of these genes, linked to metabolic pathways involving Myo-inositol, showed differential expression. Myo-inositol accumulation increased over time, suggesting its role as an osmotic regulator and reactive oxygen species (ROS) scavenger. This accumulation likely shields T. ramosissima from NaCl-induced osmotic and oxidative damage. Notably, Unigene0002140 and Unigene0095980, associated with Myo-inositol oxygenase, appear to regulate Myo-inositol accumulation and correlate significantly with its levels. We hypothesize they are key genes in controlling Myo-inositol levels, warranting further study. This research illuminates the role of Myo-inositol oxygenase-related genes in T. ramosissima roots combating NaCl stress, offering insights for selecting salt-tolerant tree species.