Effects of mycorrhizal fungi on in vitro nitrogen response of some Dutch indigenous orchid species

Dijk, Eeuwe; Eck, Nelly

doi:10.1139/b95-130

Cited by 27 publications

(16 citation statements)

References 27 publications

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“…The second gene, Unigene404_All, encodes a protein with 81% identity to LHT1 (lysine histidine transporter 1) of A. thaliana (thale cress), which was regulated by diverse factors. Our data supported the notion that OMF played an important role in improving N acquisition of host plants [ 9 , 27 ].…”

Section: Discussionsupporting

confidence: 89%

“…The plantlets with 3–4 roots about 2.5-3 cm long were then transferred to DE basal medium, an in vitro mycorrhizal induction medium in orchids [ 27 ]. The co-cultivation DE medium contained 1.0 mM CaCl 2 , 0.5 mM MgS0 4 , 1.0 mM K 2 S0 4 , 0.4 mM KH 2 P0 4 , 100 μM FeS0 4 , 25 μM H 3 BO 4 , 33 μM MnCl 2 , 2.8 μM ZnSO 4 , 1.0 μM NaMoO 4 , 140 μM Na 2 EDTA, supplemented with yeast extract 1 g · L -1 , soluble starch 9 g · L -1 and agar 6 g · L -1 .…”

Section: Methodsmentioning

confidence: 99%

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Deep sequencing–based comparative transcriptional profiles of Cymbidium hybridum roots in response to mycorrhizal and non-mycorrhizal beneficial fungi

et al. 2014

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BackgroundThe Orchidaceae is one of the largest families in the plant kingdom and orchid mycorrhizae (OM) are indispensable in the life cycle of all orchids under natural conditions. In spite of this, little is known concerning the mechanisms underlying orchid- mycorrhizal fungi interactions. Our previous work demonstrated that the non-mycorrhizal fungus Umbelopsis nana ZH3A-3 could improve the symbiotic effects of orchid mycorrhizal fungus Epulorhiza repens ML01 by co-cultivation with Cymbidium hybridum plantlets. Thus, we investigated the C. hybridum transcript profile associated with different beneficial fungi.ResultsMore than 54,993,972 clean reads were obtained from un-normalized cDNA library prepared from fungal- and mock- treated Cymbidium roots at four time points using RNA-seq technology. These reads were assembled into 16,798 unique transcripts, with a mean length of 1127 bp. A total of 10,971 (65.31%) sequences were annotated based on BLASTX results and over ninety percent of which were assigned to plant origin. The digital gene expression profiles in Cymbidium root at 15 days post inoculation revealed that 1674, 845 and 1743 genes were sigificantly regulated in response to ML01, ZH3A-3 and ML01+ ZH3A-3 treatments, respectively. Twenty-six genes in different regulation patterns were validated using quantitative RT-PCR. Our analysis showed that general defense responses were co- induced by three treatments, including cell wall modification, reactive oxygen species detoxification, secondary biosynthesis and hormone balance. Genes involved in phosphate transport and root morphogenesis were also detected to be up-regulated collectively. Among the OM specifically induced transcripts, genes related to signaling, protein metabolism and processing, defense, transport and auxin response were identifed. Aside from these orchid transcripts, some putative fungal genes were also identified in symbiotic roots related to plant cell wall degradation, remodeling the fungal cell wall and nutrient transport.ConclusionThe orchid root transcriptome will facilitate our understanding of orchid - associated biological mechanism. The comparative expression profiling revealed that the transcriptional reprogramming by OM symbiosis generally overlapped that of arbuscular mycorrhizas and ectomycorrhizas. The molecular basis of OM formation and function will improve our knowledge of plant- mycorrhzial fungi interactions, and their effects on plant and fungal growth, development and differentiation.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-747) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Deep sequencing–based comparative transcriptional profiles of Cymbidium hybridum roots in response to mycorrhizal and non-mycorrhizal beneficial fungi

et al. 2014

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“…However, there are only a few reports about nutrient application experiments with terrestrial orchids. These studies have mostly examined the effects of fertilizer treatment on the growth of terrestrial orchids in the juvenile phase (Beyrle et al 1991, Dijk andEck 1995), and have shown either no response or a negative impact on growth of corms or young plantlets after nutrient application. Negative responses of nutrient application have been reported to be due to interference on the symbiotic interactions, as high nitrogen contents caused a change of fast-growing fungi (e.g.…”

mentioning

confidence: 99%

Nutrient versus pollination limitation in Platanthera bifolia and Dactylorhiza incarnata (Orchidaceae)

Mattila

Kuitunen²

2000

Oikos

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Mattila, E. and Kuitunen, M. T. 2000. Nutrient versus pollination limitation in Platanthera bifolia and Dactylorhiza incarnata (Orchidaceae). -Oikos 89: 360 -366.Availability of resources and pollination services have been demonstrated to have impact on reproductive success in some orchid species, but to our knowledge no studies have examined the effects of nutrient application and pollination limitation in the same experiment. In this study, factors limiting reproductive success were studied with two terrestrial orchid species in Central Finland during 1996 and 1997. In a field experiment using a factorial design, plants of nectar-producing Platanthera bifolia and nectarless Dactylorhiza incarnata were treated with nutrient application and hand-pollination. Inflorescence size was considered as an indicator of attractiveness to pollinators as well as of the general condition of the plant. In 1996, fertilizer treatment increased relative capsule production in P. bifolia plants with small inflorescences, indicating poorer store of resources in the underground corm and dependence on nutrient availability during capsule maturation, which was not found in large plants. Hand-pollination and large inflorescence size in P. bifolia did not affect capsule initiation, but increased the proportion of mature capsules. P. bifolia may thus be regarded as both resource-and pollination-limited within a year. Pollination success was observed to be the only factor limiting reproduction of the nectarless D. incarnata within a year, because hand-pollination increased capsule production, and there were no significant effects of fertilizer treatment or inflorescence size in 1996. Capsule production in 1996 did not affect the probability of P. bifolia flowering in the following year, while high capsule production decreased the subsequent probability of flowering in D. incarnata. Species with different pollination strategies differed in the use of resources. The nectar-producing P. bifolia had a lower fruit/flower ratio than the nectarless D. incarnata, also after hand-pollination.

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“…Green-leaf production has also been noted in some Australian and exotic genera, including Caladenia, but without information on the time required or the proportion of green-leaf production (Harvais 1974;Clements and Ellyard 1979;Warcup 1981;Clements 1982;Clements et al 1986;Jusaitis and Sorensen 1993). Others have noted the time taken with both Australian and exotic orchids (17-80 days with fungi, longer without) but not the proportion of seeds that produced green leaves (Warcup 1971;Dijk and Eck 1995;Clements and Ellyard 1979;Milligan and Williams 1988;. Comparing fungal effectiveness in germination with other studies is also complicated by variation in the definition of germination, with some previous studies accepting protocorm swelling or rhizoid production as evidence of effectiveness (e.g.…”

Section: Fungal Isolation and Seed Germinationmentioning

confidence: 99%

Effect of developmental stage and peloton morphology on success in isolation of mycorrhizal fungi in Caladenia formosa (Orchidaceae)

Huynh¹,

McLean²,

Coates³

et al. 2004

Aust. J. Bot.

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Six developmental stages (leafing, budding, flowering, fruiting, senescence and dormancy) were chosen in the threatened terrestrial orchid Caladenia formosa G.W.Carr to optimise isolation of effective fungi. Loose (undigested) pelotons were observed by scanning electron microscopy in the old tuber and collar, suggesting a role in infection of new tissue. In collars collected at early life stages (leafing, budding, flowering), pelotons had loosely coiled hyphae that were uniformly fine (1-2 µm diameter), with or without monilioid cells. In collars collected from older life stages (fruiting, senescence), pelotons had increasing proportions (up to 94%) of clumped fine hyphae. Coarser hyphae (4-6 µm diameter) were also present in the fruiting stage in one year. Only fungi isolated from single pelotons in collars of early life stages (leafing, budding, flowering) had fine hyphae with monilioid cells and induced seed germination (to green leaf production). Sectioned protocorms had pelotons of fine, loosely coiled hyphae with monilioid cells, as in field-collected material from early life stages. This suggests that the most effective fungi for conservation of this orchid are likely to be isolated from pelotons of loose fine hyphae with monilioid cells from leafing to flowering stages. B T 0 3 0 9 9 I s o l a t i o n o f m y c o r r h i z a l f u n g i i n C a l a d e n i a f o r m o s a T . T . H u y n h e t a l .

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Effects of mycorrhizal fungi on in vitro nitrogen response of some Dutch indigenous orchid species

Cited by 27 publications

References 27 publications

Deep sequencing–based comparative transcriptional profiles of Cymbidium hybridum roots in response to mycorrhizal and non-mycorrhizal beneficial fungi

Deep sequencing–based comparative transcriptional profiles of Cymbidium hybridum roots in response to mycorrhizal and non-mycorrhizal beneficial fungi

Nutrient versus pollination limitation in Platanthera bifolia and Dactylorhiza incarnata (Orchidaceae)

Effect of developmental stage and peloton morphology on success in isolation of mycorrhizal fungi in Caladenia formosa (Orchidaceae)

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