Effects of mycophenolate sodium on mucociliary clearance using a bronchial section and anastomosis rodent model

Abstract

OBJECTIVE:To study the effects of mycophenolate sodium on mucociliary clearance.INTRODUCTION:Mycophenolate is one of the most commonly used immunosuppressive drugs in lung transplantation. Although its pharmacokinetic properties are well defined, its side effects on mucociliary clearance have not yet been studied.METHODS:Sixty rats were subjected to left bronchial section and anastomosis. The right bronchus was used as a control. After surgery, the rats were assigned to two groups based on whether they received saline solution (n = 30) or mycophenolate sodium (n = 30). After 7, 15, or 30 days of treatment, 10 animals from each group were sacrificed, and in vitro mucus transportability, in situ mucociliary transport velocity and ciliary beat frequency were measured.RESULTS:The analysis of mucus transportability revealed that neither mycophenolate nor bronchial section altered any transportability related property for up to 30 days of treatment after surgery (p>0.05). With regard to ciliary beat frequency, the operated left bronchi from the mycophenolate group showed a significant decrease on post-surgical day 30 (p = 0.003). In addition, we found a significant reduction in the in situ mucociliary transport velocity in the mycophenolate-treated group (p = 0.0001).DISCUSSION:These data add important information regarding mucociliary clearance dysfunction following mycophenolate therapy and suggest that mycophenolate might contribute to the high incidence of respiratory tract infections in lung transplant patients. Further studies are needed to investigate the combined action of mycophenolate with other immunosuppressive drugs and to establish methods to protect and recover mucociliary clearance, an important airway defense mechanism.

Prevalence and conditions associated with chronic pelvic pain in women from São Luís, Brazil

“…In contrast to what we observed in Ribeirão Preto (8), we did not observe previous abdominal surgery and low educational level as independent factors. Regarding the former, although an association is plausible, we do not know if other confounding variables or even cultural aspects may have interfered with the results.…”

“…Our two studies showed that Brazil is one of the countries with a higher prevalence of the condition, particularly in contrast to developed countries (7,8). In addition, a large part of the sample routinely used painkillers without a medical prescription, which predispose to several undesirable events, including financial expenditures without guarantee of clinical improvement.…”

“…All variables with a P<0.10 in bivariate analysis and those we considered to be plausible to justify the pain and that were significant in other studies (7,8) were included in a logistic regression model. Simple and multiple logistic regressions were used to estimate the odds ratio.…”

“…However, few survey studies worldwide have evaluated the condition. Our group observed a prevalence of approximately 11% in the Southeast of Brazil, and identified as associated independent conditions: dyspareunia, previous abdominal surgery, depression, dysmenorrhea, anxiety, current sexual activity, low back pain, constipation, irritative urinary symptoms, and low educational level (8). We believe that this information is important because our country shares many characteristics with other developing countries around the world.…”

Pesquisas em pneumologia recentemente publicadas em revistas brasileiras

“…Silva et al (63) tested the effects that mycophenolate sodium, one of the most commonly used immunosuppressive drugs in lung transplantation, has on mucociliary clearance in rats. The authors found that the drug had no effect on transportability but significantly reduced mucociliary transport velocity in situ.…”