Effects of Mustine Hydrochloride on Mouse Haematopoietic and Lymphoid Tissues

Sharp, John; Thomas, David B.; Briscoe, Cecilie V.

doi:10.1159/000221275

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…All injections were completed within 30 min of dissolving the nitrogen mustard (Merck, Sharp and Dohme, West Point, Penn.). Groups of 5-10 survivors and similar numbers of age for each of the strains and is similar to previous data reports by Sharp et al (1973).…”

Section: Methodssupporting

confidence: 90%

Inverse Relationship between Splenomegaly and Stem Cell Compartment Size in Mice Treated with Nitrogen Mustard

Jensen

Sharp

Zajic

et al. 1977

Scandinavian Journal of Haematology

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Following the administration of similar doses of nitrogen mustard (4 mg/kg) to different strains of mice, wide variations in the subsequent degree of splenomegaly were observed, implying strain differences in the role of the spleen in the compensatory erythropoietic response to haematopoietic stress. This investigation was undertaken to determine whether or not these differences were related to the size of the haematopoietic stem cell compartment size in the various strains of mice. Groups of 4 different strains of mice (Swiss Webster, A/J, C57BL/6J and CS1/ASH) were injected i.v. with nitrogen mustard (4 mg/kg body weight) and autopsied at regular intervals up to 20 d post‐injection. At autopsy, the wet weight of the spleen was determined. Subsequently, groups of the same 4 strains of mice were exposed to single doses of wholebody γ‐irradiation in the range of 500–900 rads. 9 d after γ‐irradiation the mice were autopsied, their spleens removed, and the number of endogenous spleen colonies determined. The greatest degree of splenomegaly was observed in the C57bl/6J mice. The Swiss Webster mice showed no splenomegaly during the time period studied. There existed a linear inverse relationship between the maximum degree of splenomegaly observed and the dose of wholebody γ‐irradiation required to completely eliminate endogenous spleen colonies. This data is in accord with the hypothesis that there exists an inverse relationship between the extent of splenomegaly observed following haematopoietic stress and the haematopoietic stem cell compartment size.

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Section: Methodssupporting

confidence: 90%

Inverse Relationship between Splenomegaly and Stem Cell Compartment Size in Mice Treated with Nitrogen Mustard

Jensen

Sharp

Zajic

et al. 1977

Scandinavian Journal of Haematology

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References

Lohrmann¹,

Schreml²

1982

Cytotoxic Drugs and the Granulopoietic System

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Prolonged Effects of Nitrogen Mustard on Mouse Haematopoietic and Lymphoid Tissues

Sharp

Thomas

Briscoe

et al. 1975

Scandinavian Journal of Haematology

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Groups of mice have been autopsied at regular intervals during the period of lymphomyeloid tissue regeneration which follows the phase of hypocellularity induced by the i.v. injection of 100 μg (4 mg/kg bodyweight) nitrogen mustard. The marrow cellularity recovered to levels in the normal range by the 8th day and remained in this range up to the 40th day. Subsequently, the marrow showed a slight degree of hypocellularity up to day 120. Granulocytes were predominant during the initial phase of marrow regeneration from days 5–12. The thymus, lymph nodes, and spleen commenced regeneration during the second week post‐injection. The thymus exhibited periodic size variations such that it was substantially larger than the thymus of age‐matched controls from 20–30 d, 46–73 d, and 75–100 d after injection. The lymph nodes and lymphoid tissue of the spleen regenerated only slowly to reach control values by 40–50 d. Superimposed on the recovering lymphoid tissue of the spleen was a phase of erythroid hyperplasia lasting from 10–18 d post‐injection. This coincided with a shift from granulocytosis to erythroid hyperplasia in the marrow. This erythroid hyperplasia lasted until day 30 when the cellular composition of the marrow and spleen returned to normal. A possible explanation of these results is that nitrogen mustard introduces a degree of synchrony into stem cell proliferation and differentiation. Additionally, these results emphasize the role of the haematopoietic microenvironment in the control of stem cell differentiation.

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Effects of Mustine Hydrochloride on Mouse Haematopoietic and Lymphoid Tissues

Cited by 3 publications

References 7 publications

Inverse Relationship between Splenomegaly and Stem Cell Compartment Size in Mice Treated with Nitrogen Mustard

Inverse Relationship between Splenomegaly and Stem Cell Compartment Size in Mice Treated with Nitrogen Mustard

References

Prolonged Effects of Nitrogen Mustard on Mouse Haematopoietic and Lymphoid Tissues

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