Effects of multiple freeze thaws and various temperatures on the reactivity of human immunodeficiency virus antibody using three detection assays

Fipps, Donald R.; Damato, James J.; Brandt, Brenda L.; Burke, Donald S.

doi:10.1016/0166-0934(88)90146-2

Cited by 16 publications

(12 citation statements)

References 3 publications

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“…A larger sample size might provide stronger statistical support. These results are similar to those reported previously for the stability of samples used for testing with HIV-1 antibody assays for the diagnosis of HIV-1 infection [12]. In that study, repeated freeze-thaws and incubation at −20°C and 4°C did not alter the capacity of ELISA and Western blot assays to detect antibodies to HIV.…”

Section: Discussionsupporting

confidence: 91%

“…A small decline in antibody reactivity was noted when samples were incubated at 25°C or 37°C for more than 21 days; however, that change did not alter the interpretation of the test results. [12]. Our study extends the previous study by evaluating the effect of freeze-thaws and incubation at different temperatures on the performance of assays that measure different characteristics of the anti-HIV antibody response: the proportion of antibody that is HIV-specific, antibody titer, and antibody avidity.…”

Section: Discussionmentioning

confidence: 56%

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The Effect of Sample Handling on Cross Sectional HIV Incidence Testing Results

Laeyendecker

Latimore²,

Eshleman³

et al. 2011

PLoS ONE

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Objective(s)To determine if mishandling prior to testing would make a sample from a chronically infected subject appear recently infected when tested by cross-sectional HIV incidence assays.MethodsSerum samples from 31 subjects with chronic HIV infection were tested. Samples were subjected to different handling conditions, including incubation at 4°C, 25°C and 37°C, for 1, 3, 7 or 15 days prior to testing. Samples were also subjected to 1,3, 7 and 15 freeze-thaw cycles prior to testing. Samples were tested using the BED capture enzyme immuno assay (BED-CEIA), Vironostika-less sensitive (V-LS), and an avidity assay using the Genetic Systems HIV-1/HIV-2 plus O EIA (avidity assay).ResultsCompared to the sample that was not subjected to any mishandling conditions, for the BED-CEIA, V-LS and avidity assay, there was no significant change in test results for samples incubated at 4°C or 25°C prior to testing. No impact on test results occurred after 15 freeze-thaw cycles. A decrease in assay results was observed when samples were held for 3 days or longer at 37°C prior to testing.ConclusionsSamples can be subjected up to 15 freeze-thaw cycles without affecting the results the BED-CEIA, Vironostika-LS, or avidity assays. Storing samples at 4°C or 25°C for up to fifteen days prior to testing had no impact on test results. However, storing samples at 37°C for three or more days did affect results obtained with these assays.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 56%

The Effect of Sample Handling on Cross Sectional HIV Incidence Testing Results

Laeyendecker

Latimore²,

Eshleman³

et al. 2011

PLoS ONE

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“…Results could also be affected by the prolonged storage in -20° C before analyses, and for albumin, total concentrations of IgM, IgG and C6 antibodies samples had been refrozen and thawed before analysis. However, all samples were handled in the same way and previous studies indicate that serum proteins such as albumin and immunoglobulins are fairly robust and insensitive to storage in -20° C during time intervals such as in this study as well as to repeated freeze-thaw cycles (29)(30)(31)(32). According to our limited investigation we found the precision to be less than that reported by the manufacturer regarding CXCL13.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic performance of cerebrospinal fluid chemokine CXCL13 and antibodies to the C6-peptide in Lyme neuroborreliosis

Tjernberg

Henningsson

Eliasson

et al. 2011

Journal of Infection

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“…In addition to concerns with the extension of storage process of biological materials, many researchers (4,9,15) are interested in checking the effect of multiple consecutive cycles of freezing and thawing on antibody reactivity, since this procedure may cause occasional change in results of a particular test. In this study it was not possible to have evidence of the number of freezing and thawing cycles of analyzed samples, but the relevance of this aspect may be decreased, as found in Castejon et al (2012) study (4) , which did not observed adverse effects on the reactivity of positive samples for detection of anti-HIV antibodies, without false-negative occurrence reaction, either false-positive results in negative samples for HIV, after multiple cycles of sera freezing and thawing.…”

Section: Discussionmentioning

confidence: 99%

“…Due to scarcity of available data on which adverse effect the long-term storage may cause in the antibodies configuration, protein structures that are inclined to denaturation, it is particularly relevant to conduct a research through implementation of sensitive and specific assays. The concern with the storage time of the serum is related to various factors, such as temperature and samples freezing and thawing processes, which may cause occasional changes in the results of a particular test due to physical decay and, consequently, the biochemical modifications of antibodies of interest (3,4,9,10,15) .…”

mentioning

confidence: 99%

Stability of anti-HIV antibodies in serum samples stored for two to eighteen years periods

Castejon¹,

Yamashiro²,

Oliveira

et al. 2014

Jornal Brasileiro De Patologia E Medicina Laboratorial

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Introduction: The antibodies have an important role in the serodiagnosis, constituting the most widely used biomarkers to detect and confirm various diseases. Objective: To investigate the reproducibility of anti-human immunodeficiency virus (HIV) antibodies reactivity, to assess the stability of the sera samples stored at -20ºC for two to eighteen years. Method: Sera were collected in the period 1988-2004 for routine anti-HIV antibodies diagnostic testing. The remaining samples stored at -20ºC, were analyzed in this study. Serum sample stability was assessed by enzyme-linked immunosorbent assay/enzyme immunoassay (ELISA/EIA), indirect immunofluorescence assay (IFA), and Western blot (WB) for detecting anti-HIV antibodies. The previously found results (1988)(1989)(1990)(1991)(1992)(1993)(1994)(1995)(1996)(1997)(1998)(1999)(2000)(2001)(2002)(2003)(2004) and those obtained in 2006 were subjected to Kappa index analysis. Result: In the period 1988-to 2004, the degree of concordance of the ELISA/EIA, IFA and WB results were considered, good (k = 0.80), regular (k = 0.35), and good (k = 0.63), respectively. Conclusion: Regarding HIV serologic test, the serum samples were stable for 18 years in ELISA/EIA and for 4 years in IFA technique, however, for the WB methodology it was not possible to determine the time of stability of the anti-HIV antibodies.

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Effects of multiple freeze thaws and various temperatures on the reactivity of human immunodeficiency virus antibody using three detection assays

Cited by 16 publications

References 3 publications

The Effect of Sample Handling on Cross Sectional HIV Incidence Testing Results

The Effect of Sample Handling on Cross Sectional HIV Incidence Testing Results

Diagnostic performance of cerebrospinal fluid chemokine CXCL13 and antibodies to the C6-peptide in Lyme neuroborreliosis

Stability of anti-HIV antibodies in serum samples stored for two to eighteen years periods

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